Sequence analysis of two porcine rotaviruses differing in growth and in pathogenicity: distinct VP4 sequences and conservation of NS53, VP6 and VP7 genes Free

Abstract

The VP4, VP7, NS53 and VP6 genes of two porcine rotavirus variants which differ in their growth properties and pathogenicity have been cloned and sequenced. The VP4 genes show only 67·2% nucleic acid and 70·6% amino acid identity. The VP4 gene of one variant (4S) is closely related to that of the bovine UK rotavirus strain, whereas the VP4 gene of the other variant (4F) is only distantly related to known VP4 genes and is likely to represent a new P serotype. In contrast the NS53 (VP5), VP6 and VP7 genes of the 4F and 4S variants show greater than 99 % nucleotide and amino acid identity, indicating that the two viruses are genetically related by a reassortment event. The implications for the role of VP4 in the determination of growth characteristics and pathogenicity are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-9-2205
1994-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/9/JV0750092205.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-9-2205&mimeType=html&fmt=ahah

References

  1. Bellinzoni R. B., Mattion N. M., Matson D. M., Blackhall J., Latorre J. L., Scodeller E. A., Urasawa S., Taniguchi K., Estes M. K. 1990; Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. Journal of Clinical Microbiology 28:633–636
    [Google Scholar]
  2. Both G. W., Mattick J. S., Bellamy A. R. 1983; Serotype- specific glycoprotein of simian rotavirus: coding assignment and gene sequence. Proceedings of the National Academy of Sciences U.S.A: 803091–3095
    [Google Scholar]
  3. Both G. W., Siegman L. J., Bellamy A. R., Ikegami N., Shatkin A. J., Furuichi Y. 1984; Comparative sequence analysis of rotavirus genomic segment 6 - the gene specifying viral subgroups 1 and 2. Journal of Virology 51:97–101
    [Google Scholar]
  4. Bridger J. C., Burke B., Beards G. M., Desselberger U. 1992; The pathogenicity of two porcine rotaviruses differing in their in vitro growth characteristics and genes 4. Journal of General Virology 73:3011–3015
    [Google Scholar]
  5. Broome R. L., Vo P. T., Ward R. L., Clark H. F., Greenberg H. B. 1993; Murine rotavirus genes encoding outer capsid proteins VP4 and VP7 are not major determinants of host range restriction and virulence. Journal of Virology 67:2448–2455
    [Google Scholar]
  6. Browning G. F., Chalmers R. M., Fitzgerald T. A., Snodgrass D. R. 1991a; Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. Journal of General Virology 72:1059–1064
    [Google Scholar]
  7. Browning G. F., Fitzgerald T. A., Chalmers R. M., Snodgrass D. R. 1991b; A novel group A rotavirus serotype: serological and genomic characterisation of equine isolate FI23. Journal of Clinical Microbiology 29:2043–2046
    [Google Scholar]
  8. Chen D., Burns J. W., Estes M. K., Ramig R. F. 1989; Phenotypes of rotavirus reassortants depend upon the recipient genetic background. Proceedings of the National Academy of Sciences U.S.A: 863743–3747
    [Google Scholar]
  9. Chen D., Estes M., Ramig R. F. 1992; Specific interactions between rotavirus outer capsid proteins VP4 and VP7 determine expression of a cross-reactive neutralising VP4-specific epitope. Journal of Virology 66:432–439
    [Google Scholar]
  10. Clark S. M., Roth J. R., Clark M. L., Barnett B. B., Spendlove R. S. 1981; Trypsin enhancement of rotavirus in- fectivity: mechanism of enhancement. Journal of Virology 39:816–822
    [Google Scholar]
  11. Das M., Dunn S. J., Woode G. N., Greenberg H. B., Rao C. D. 1993; Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (1321) have high levels of sequence identity with the homologous proteins of a serotype 10 bovine rotavirus. Virology 194:374–379
    [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  13. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. 1983; Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Research 11:4689–4701
    [Google Scholar]
  14. Estes M. K. 1990; Rotaviruses and their replication. In Virology,, 2nd edn.. pp 1329–1352 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  15. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–499
    [Google Scholar]
  16. Estes M. K., Graham D. Y., Mason B. B. 1981; Proteolytic enhancement of rotavirus infection: molecular mechanisms. Journal of Virology 39:879–888
    [Google Scholar]
  17. Gentsch J. R., Das B. K., Jiang B., Bhan M. K., Glass R. I. 1993; Similarity of the VP4 protein of human rotavirus strain 116E to that of the bovine B223 strain. Virology 194:424–430
    [Google Scholar]
  18. Gorziglia M., Aguirre Y., Hoshino Y., Esparza J., Blumentals I., Askaa J., Thompson M., Glass R. I., Kapikian A. Z., Chanock R. M. 1986; VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence. Journal of General Virology 67:2445–2454
    [Google Scholar]
  19. Gorziglia M., Green K., Nishikawa K., Taniguchi K., Jones R., Kapikian A. Z., Chanock R. M. 1988a; Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. Journal of Virology 64:2978–2984
    [Google Scholar]
  20. Gorziglia M., Hoshino Y., Nishikawa K., Maloy W. L., Jones R. W., Kapikian A. Z., Chanock R. M. 1988b; Comparative sequence analysis of the genome segment 6 of four rotaviruses each with a different subgroup specificity. Journal of General Virology 69:1659–1669
    [Google Scholar]
  21. Gorziglia M., Nishikawa K., Green K., Taniguchi K. 1988c; Gene sequence of the VP7 serotype specific glycoprotein of Gottfried porcine rotavirus. Nucleic Acids Research 16:775
    [Google Scholar]
  22. Gorziglia M., Larralde G., Kapikian A. Z., Chanock R. M. 1990a; Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proceedings of the National Academy of Sciences U.S.A: 877155–7159
    [Google Scholar]
  23. Gorziglia M., Nishikawa K., Hoshino Y., Taniguchi K. 1990b; Similarity of the outer capsid protein VP4 of the Gottfried strain of porcine rotavirus to that of asymptomatic human rotavirus strains. Journal of Virology 64:414–418
    [Google Scholar]
  24. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. 1987; Comparison of the amino acid sequences of the major neutralisation protein of four human rotavirus serotypes. Virology 161:153–159
    [Google Scholar]
  25. Green K. Y., Hoshino Y., Ikegami N. 1989; Sequence analysis of the gene encoding the serotype-specific glycoprotein of two new human rotavirus serotypes. Virology 168:429–433
    [Google Scholar]
  26. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. 1983; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  27. Gunn P. R., Sato F., Powell K. F. H., Bellamy A. R., Napier J. R., Harding D. R. K., Hancock W. S., Siegman L. J., Both G. W. 1985; Rotavirus neutralising protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis. Journal of Virology 54:791–797
    [Google Scholar]
  28. Haddow J., Clark B., Ni Y. W., Desselberger U. 1989; Biological function of the porcine rotavirus protein VP4: observations on porcine isolates from China. Medical Microbiology and Immunology 178:163–176
    [Google Scholar]
  29. Hardy M. E., Woode G. N., Xu Z., Gorziglia M. 1991; Comparative amino acid sequence analysis of VP4 for VP7 serotype 6 bovine rotavirus strains NCDV, B641, and UK. Journal of Virology 65:5535–5538
    [Google Scholar]
  30. Hardy M. E., Gorziglia M., Woode G. N. 1992; Amino acid analysis of bovine rotavirus B223 reveals a unique outer capsid protein VP4 and confirms a third bovine VP4 type. Virology 191:291–300
    [Google Scholar]
  31. Hardy M. E., Gorziglia M., Woode G. N. 1993; The outer capsid protein VP4 of equine rotavirus strain H-2 represents a unique VP4 type by amino acid sequence analysis. Virology 193:492–497
    [Google Scholar]
  32. Hua J., Mansell E. A., Patton J. T. 1993; Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA. Virology 196:372–378
    [Google Scholar]
  33. Huang J. A., Nagesha H. S., Snodgrass D. R., Holmes I. H. 1992; Molecular and serological analyses of two bovine rotaviruses (B-11 and B-60) causing calf scours in Australia. Journal of Clinical Microbiology 30:85–92
    [Google Scholar]
  34. Hum C. P., Dyall-Smith M. L., Holmes I. H. 1989; The VP7 gene of a new G serotype of human rotavirus (B37) is similar to G3 proteins in the antigenic C region. Virology 170:55–61
    [Google Scholar]
  35. Isegawa Y., Nakagomi O., Nakagomi T., Ueda S. 1992; A VP4 sequence highly conserved in human rotavirus strain AU-1 and feline rotavirus strain FRV-1. Journal of General Virology 73:1939–1946
    [Google Scholar]
  36. Kalica A. R., Flores J., Greenberg H. B. 1983; Identification of the rotavirus gene which codes for the hemagglutinin and protease- enhanced plaque formation. Virology 125:194–205
    [Google Scholar]
  37. Kantharidis P., Dyall-Smith M. L., Holmes I. H. 1987; Marked sequence variation between segment 4 genes of human RV5 and simian SA11 rotaviruses. Archives of Virology 93:111–121
    [Google Scholar]
  38. Kantharidis P., Dyall-Smith M. L., Tregear G. W., Holmes I. H. 1988; Nucleotide sequence of UK bovine rotavirus segment 4: possible host restriction of VP3 genes. Virology 166:308–315
    [Google Scholar]
  39. Kapikian A. Z., Chanock R. M. 1990; Rotaviruses. In Virology, 2nd edn.. pp 1353–1404 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  40. Kool D. A., Matsui S. M., Greenberg H. B., Holmes I. H. 1992; Isolation and characterization of a novel reassortant between avian TY-1 and simian RRV rotaviruses. Journal of Virology 66:6836–6839
    [Google Scholar]
  41. López S., Arias C. F. 1993; Sequence analysis of rotavirus YM VP6 and NS28 proteins. Journal of General Virology 74:1233–1226
    [Google Scholar]
  42. López S., Arias C. F., Bell J. R., Strauss J. H., Espejo R. T. 1985; Primary structure of the cleavage site associated with trypsin enhancement of rotavirus SA11 infectivity. Virology 144:11–19
    [Google Scholar]
  43. López S., López I., Romero P., Mendez E., Soberon X., Arias C. F. 1991; Rotavirus YM gene 4: analysis of its deduced amino acid sequence and prediction of the secondary structure of the VP4 protein. Journal of Virology 65:3738–3745
    [Google Scholar]
  44. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Dang M., Greenberg H. B. 1988; The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous neutralization and identification of a putative fusion region. Proceedings of the National Academy of Sciences U.S.A: 85645–649
    [Google Scholar]
  45. Mitchell D. B., Both G. W. 1989; Complete nucleotide sequence of the simian rotavirus SA11 VP4 gene. Nucleic Acids Research 17:2122
    [Google Scholar]
  46. Ni Y. W. 1986; Isolation and identification of porcine and bovine rotavirus. Chinese Journal of Virology 2:36–40
    [Google Scholar]
  47. Nishikawa K., Gorziglia M. 1988; The nucleotide sequence of the VP3 gene of porcine rotavirus OSU. Nucleic Acids Research 16:11847
    [Google Scholar]
  48. Nishikawa K., Hoshino Y., Gorziglia M. 1991; Sequence of the VP7 gene of chicken rotavirus Ch2 strain of serotype 7 rotavirus. Virology 185:853–857
    [Google Scholar]
  49. Offit P. A., Blavat G., Greenberg H. B., Clark H. F. 1986; . Molecular basis for rotavirus virulence: role of rotavirus gene segment 4, Journal of Virology 57:46–49
    [Google Scholar]
  50. Patton J. T., Hua J., Mansell E. A. 1993; Location ofintrachain disulfide bonds in the VP5* and VP8* trypsin cleavage fragments of the Rhesus rotavirus spike protein VP4. Journal of Virology 67:4848–4855
    [Google Scholar]
  51. Potter A. A., Cox G., Parker M., Babiuk L. A. 1987; The complete nucleotide sequence of bovine rotavirus C486 gene 4 cDNA. Nucleic Acids Research 15:4361
    [Google Scholar]
  52. Prasad B. V. V., Wang G. J., Clerx J. P. M., Chiu W. 1988; Three dimensional structure of rotavirus. Journal of Molecular Biology 199:269–275
    [Google Scholar]
  53. Prasad B. V. V., Burns J. W., Marietta E., Estes M. K., Chiu W. 1990; Localization of VP4 neutralization sites in rotavirus by three-dimensional cryoelectron microscopy. Nature; London: 343476–479
    [Google Scholar]
  54. Qian Y., Green K. Y. 1991; Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology 182:407–412
    [Google Scholar]
  55. Richardson M. A., Iwamoto A., Ikegami N., Nomoto A., Furuichi Y. 1984; Nucleotide sequence of the gene encoding the serotype-specificity of human (Wa) rotavirus: comparison with the homologous gene from simian SA11 and UK bovine rotaviruses. Journal of Virology 51:860–862
    [Google Scholar]
  56. Ruggeri F. M., Greenberg H. B. 1991; Antibodies to the trypsin cleavage peptide VP8* neutralize rotavirus by inhibiting binding of virions to target cells in cell culture. Journal of Virology 65:2211–2219
    [Google Scholar]
  57. Ruiz A. M., López I. V., López S., Espejo R. T., Arias C. F. 1988; Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. Journal of Virology 62:4331–4336
    [Google Scholar]
  58. Shen S., Burke B., Desselberger U. 1993; Nucleotide sequences of the VP4 and VP7 genes of a Chinese lamb rotavirus: evidence for a new P type in a G10 type virus. Virology 197:497–500
    [Google Scholar]
  59. Snodgrass D. R., Hoshino Y., Fitzgerald T. A., Smith M., Browning G. F., Gorziglia M. 1992; Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral reassortants. Journal of General Virology 73:2319–2325
    [Google Scholar]
  60. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  61. Staden R. 1984; A computer program to enter DNA gel reading data into a computer. Nucleic Acids Research 12:499–503
    [Google Scholar]
  62. Taniguchi K., Maloy W. L., Nishikawa K., Green K. Y., Hoshino Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988; Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. Journal of Virology 62:2421–2426
    [Google Scholar]
  63. Taniguchi K., Nishikawa K., Urasawa T., Urasawa S., Midthun K., Kapikian A. Z., Gorziglia M. 1989; Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. Journal of Virology 63:4101–4106
    [Google Scholar]
  64. Taniguchi K., Urasawa T., Kobayashi N., Gorziglia M., Urasawa S. 1990; Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. Journal of Virology 64:5640–5644
    [Google Scholar]
  65. Winship P. R. 1989; An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Research 17:1266
    [Google Scholar]
  66. Xu L., Harbour D., Mccrae M. A. 1990; The application of polymerase chain reaction to the detection of rotaviruses in faeces. Journal of Virological Methods 27:29–38
    [Google Scholar]
  67. Xu L., Harbour D., Mccrae M. A. 1991; Sequence of the gene encoding the major neutralisation antigen (VP7) of serotype 10 rotavirus. Journal of General Virology 72:177–180
    [Google Scholar]
  68. Zhang H., Scholl R., Browse J., Somerville C. 1988; Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Research 16:1220
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-9-2205
Loading
/content/journal/jgv/10.1099/0022-1317-75-9-2205
Loading

Data & Media loading...

Most cited Most Cited RSS feed