1887

Abstract

Brefeldin A (BFA) is a macrolide antibiotic that blocks membrane traffic through the vesicular system and affects the glycosylation of viral glycoproteins. Treatment of HeLa cells infected with Semliki Forest virus (SFV) with BFA enhances the synthesis of late viral proteins. Proteolytic cleavage of p107 is partially blocked and viral glycoproteins accumulate in BFA-treated cells. This enhanced synthesis of late SFV proteins is due, at least in part, to an increase in the formation of the subgenomic SFV 26S mRNA. Since BFA blocks the replication of poliovirus genomes without affecting the cleavage of the translation initiation factor p220, protein synthesis was analysed in doubly infected cells. HeLa cells infected with SFV and poliovirus at the same multiplicity predominantly synthesize poliovirus proteins. But if these cells are treated with BFA they synthesize significant amounts of SFV capsid protein C for several hours, despite the fact that p220 has been degraded.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-9-2197
1994-09-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/9/JV0750092197.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-9-2197&mimeType=html&fmt=ahah

References

  1. Acheson N. H., Tamm I. 1967; Replication of Semliki Forest virus: an electron microscopic study. Virology 32:128–143
    [Google Scholar]
  2. Alcalde J., Bonay P., Roa A., Vilaro S., Sandoval I. V. 1992; Assembly and disassembly of the Golgi complex: two processes arranged in a cis-trans direction. Journal of Cell Biology 116:69–83
    [Google Scholar]
  3. Alonso M. A., Carrasco L. 1982a; Translation of capped viral mRNAs in poliovirus-infected FleLa cells. EMBO Journal 1:913–917
    [Google Scholar]
  4. Alonso M. A., Carrasco L. 1982b; Protein synthesis in HeLa cells double-infected with encephalomyocarditis virus and poliovirus. Journal of General Virology 61:15–24
    [Google Scholar]
  5. Bonneau A. M., Sonenberg N. 1987; Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection. Journal of Virology 61:986–991
    [Google Scholar]
  6. Carrasco L. 1978; Membrane leakiness after viral infection and a new approach to the development of antiviral agents. Nature; London: 272694–699
    [Google Scholar]
  7. Carrasco L., Castrillo J. L. 1987; The regulation of translation in picornavirus-infected cells. In Mechanisms of Viral Toxicity in Animal Cells, pp 115–146 Carrasco L. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  8. Carrasco L., Harvey R., Blanchard C., Smith A. E. 1979; Regulation of translation of eukaryotic virus mRNAs. Haematologie und Bluttransfusion 23:277–281
    [Google Scholar]
  9. Carrasco L., Otero M. J., Castrillo J. 1989; Modification of membrane permeability by animal viruses. Pharmacology and Therapeutics 40:171–212
    [Google Scholar]
  10. Carrasco L., Pérez L., Irurzun A., Lama J., Martínez-Abarca F., Rodrígez P., Guinea R., Castrillo J. L., Sanz M. A., Ayala M. J. 1993; Modification of membrane permeability by animal viruses. In Regulation of Gene Expression in Animal viruses pp 283–303 Carrasco L., Sonenberg N., Wimmer E. Edited by New York: Plenum Press;
    [Google Scholar]
  11. Castrillo J. L., Vanden-Berghe D., Carrasco L. 1986; 3- Methylquercetin is a potent and selective inhibitor of poliovirus RNA synthesis. Virology 152:219–227
    [Google Scholar]
  12. Chege N. W., Pfeffer S. R. 1990; Compartmentation of the Golgi complex: brefeldin-A distinguishes trans-Golgi cisternae from the trans-Golgi network. Journal of Cell Biology 111:893–899
    [Google Scholar]
  13. Chen S., Matsuoka Y., Compans R. W. 1991; Assembly and polarized release of Punta Toro virus and effects of brefeldin A. Journal of Virology 65:1427–1439
    [Google Scholar]
  14. Cheung P., Banfield B. W., Tufaro F. 1991; Brefeldin A arrests the maturation and egress of herpes simplex virus during infection. Journal of Virology 65:1893–1904
    [Google Scholar]
  15. Collins P. L., Mottet G. 1992; Oligomerization and posttranslational processing of glycoprotein G of human respiratory syncytial virus: altered O-glycosylation in the presence of brefeldin A. Journal of General Virology 13:849–863
    [Google Scholar]
  16. Davies M. V., Pelletier J., Meerovitch K., Sonenberg N., Kaufman R. J. 1991; The effect of poliovirus proteinase 2Apro on cellular metabolism. Journal of Biological Chemistry 266:14714–14720
    [Google Scholar]
  17. De Curtis I., Simons K. 1988; Dissection of Semliki Forest virus glycoprotein delivery from the trans-Golgi network to the cell surface in permeabilized BHK cells. Proceedings of the National Academy of Sciences U.S.A: 858052–8056
    [Google Scholar]
  18. Etchison D., Etchison J. R. 1987; Monoclonal antibody-aided characterization of cellular p220 in uninfected and poliovirus- infected HeLa cells: subcellular distribution and identification of conformers. Journal of Virology 61:2702–2710
    [Google Scholar]
  19. Etchison D., Milburn S. C., Edery I., Sonenberg N., Hershey J. W. 1982; Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex. Journal of Biological Chemistry 257:14806–14810
    [Google Scholar]
  20. Froshauer S., Kartenbeck J. 1988; Alpha virus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. Journal of Cell Biology 107:2075–2086
    [Google Scholar]
  21. Garry R. F., Bishop J. M., Parker S., Westbrook K., Lewis G., Waite M. R. 1979; Na+ and K+ concentrations and the regulation of protein synthesis in Sindbis virus-infected chick cells. Virology 96:108–120
    [Google Scholar]
  22. Griffiths G., Simons K. 1986; The trans Golgi network: sorting at the exit site of the Golgi complex. Science 234:438–443
    [Google Scholar]
  23. Griffiths G., Fuller S. D., Back R., Hollinshead M., Pffeifer S., Simons K. 1989; The dynamic nature of the Golgi complex. Journal of Cell Biology 108:277–297
    [Google Scholar]
  24. Guinea R., Carrasco L. 1990; Phospholipid biosynthesis and poliovirus genome replication, two coupled phenomena. EMBO Journal 9:2011–2016
    [Google Scholar]
  25. Helms J. B., Rothman J. E. 1992; Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature; London: 360352–354
    [Google Scholar]
  26. Hunziker W., Whitney J. A., Mellman I. 1992; Brefeldin A and the endocytic pathway: possible implications for membrane traffic and sorting. FEBS Letters 307:93–96
    [Google Scholar]
  27. Irurzun A., Perez L., Carrasco L. 1992; Involvement of membrane traffic in the replication of poliovirus genomes: effects of Brefeldin A. Virology 191:166–175
    [Google Scholar]
  28. Klausner R. D., Donaldson J. G., Lippincott-Schwartz J. 1992; Brefeldin A: insights into the control of membrane traffic and organelle structure. Journal of Cell Biology 116:1071–1080
    [Google Scholar]
  29. Ladinsky M. S., Howell K. E. 1992; The trans-Golgi network can be dissected structurally and functionally from the cisternae of the Golgi complex by brefeldin A. European Journal of Cell Biology 59:92–105
    [Google Scholar]
  30. Lippincott-Schwartz J. 1993; Bidirectional membrane traffic between the endoplasmic reticulum and Golgi apparatus. Trends in Cell Biology 3:81–88
    [Google Scholar]
  31. Lloyd R. E., Bovee M. 1993; Persistent infection of human erythroblastoid cells by poliovirus. Virology 194:200–209
    [Google Scholar]
  32. Maynell L. A., Kirkegaard K., Klymkowsky M. W. 1992; Inhibition of poliovirus RNA synthesis by brefeldin A. Journal of Virology 66:1985–1994
    [Google Scholar]
  33. Melancon P., Garoff H. 1987; Processing of the Semliki Forest virus structural polyprotein: role of the capsid protease. Journal of Virology 61:1301–1309
    [Google Scholar]
  34. Misumi Y., Miki K., Takatsuki A., Tamura G., Ikehara Y. 1986; Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes. Journal of Biological Chemistry 261:11398–11403
    [Google Scholar]
  35. Muñoz A., Alonso M. A., Carrasco L. 1984; Synthesis of heat-shock proteins in HeLa cells: inhibition by virus infection. Virology 137:150–159
    [Google Scholar]
  36. Muñoz A., Alonso M. A., Carrasco L. 1985a; The regulation of translation in reovirus-infected cells. Journal of General Virology 66:2161–2170
    [Google Scholar]
  37. Muñoz A., Castrillo J. L., Carrasco L. 1985b; Modification of membrane permeability during Semliki Forest virus infection. Virology 146:203–212
    [Google Scholar]
  38. Oda K., Fujiwara T., Ikehara Y. 1990; . Brefeldin A arrests the intracellular transport of viral envelope proteins in primary cultured rat hepatocytes and HepG2 cells Biochemical Journal 265:161–167
    [Google Scholar]
  39. Pérez L., Carrasco L. 1991; Cerulenin-an inhibitor of lipid synthesis - blocks vesicular stomatitis virus RNA replication. FEBS Letters 280:129–133
    [Google Scholar]
  40. Pérez L., Carrasco L. 1992; Lack of direct correlation between p220 cleavage and the shut-off of host translation after poliovirus infection. Virology 189:178–186
    [Google Scholar]
  41. Pérez L., Guinea R., Carrasco L. 1991; Synthesis of Semliki Forest virus RNA requires continuous lipid synthesis. Virology 183:74–82
    [Google Scholar]
  42. Rothman J. E., Orci L. 1992; Molecular dissection of the secretory pathway. Nature; London: 355409–415
    [Google Scholar]
  43. Schlesinger M. J., London S. D., Ryan C. 1993; An in-frame insertion into the Sindbis virus 6K gene leads to defective proteolytic processing of the virus glycoproteins, a Irani-dominant negative inhibition of normal virus formation, and interference in virus shut off of host-cell protein synthesis. Virology 193:424–432
    [Google Scholar]
  44. Schrader A. P., Westaway E. G. 1990; Successful competition in translation by the flavivirus Kunjin with poliovirus during coinfections in Vero cells. Archives of Virology 114:75–89
    [Google Scholar]
  45. Sonenberg N. 1990; Poliovirus translation. Current Topics in Microbiology and Immunology 161:22–41
    [Google Scholar]
  46. Ulmer J. B., Palade G. E. 1990; Effects of brefeldin A on the processing of viral envelope glycoproteins in murine erythroleukemia cells. Journal of Biological Chemistry 266:9173–9179
    [Google Scholar]
  47. Ulug E. T., Garry R. F., Waite M. R., Bose H. R. J. 1984; Alterations in monovalent cation transport in Sindbis virus-infected chick cells. Virology 132:118–130
    [Google Scholar]
  48. Van Steeg H., Kasperaitis M., Voorma H. O., Benne R. 1984; Infection of neuroblastoma cells by Semliki Forest virus. The interference of viral capsid protein with the binding of host messenger RNAs into initiation complexes is the cause of the shut-off of host protein synthesis. European Journal of Biochemistry 138:473–478
    [Google Scholar]
  49. Whealy M. E., Card J. P., Meade R. P., Robbins A. K., Enquist L. W. 1991; Effect of brefeldin A on alphaherpesvirus membrane protein glycosylation and virus egress. Journal of Virology 65:1066–1081
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-9-2197
Loading
/content/journal/jgv/10.1099/0022-1317-75-9-2197
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error