1887

Abstract

Replicase-mediated resistance represents a genetically engineered form of resistance against viral infections and is established by using an intact or modified viral polymerase (replicase) gene for the transformation of the host. The K strain of cucumber mosaic cucumovirus (K-CMV) has been shown to break replicase-mediated resistance generated using a modified RNA2 of the subgroup I strain Fny-CMV. A full-length cDNA clone of K-CMV RNA2 was generated and RNA transcripts were shown to be infectious in combination with Fny-CMV RNAs 1 and 3. We further demonstrate that the ability of K-CMV to overcome resistance in the transgenic plants maps to RNA2. The complete nucleotide sequence of this RNA was determined and sequence differences from Fny-CMV RNA2 are discussed with regard to functional domains and their possible involvement in resistance breakage.

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1994-08-01
2022-05-28
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References

  1. Audy P., Palukaitis P., Slack S. A., Zaitlin M. 1994; Replicase-mediated resistance to potato virus Y in transgenic tobacco plants. Molecular Plant-Microbe Interactions 7:15–22
    [Google Scholar]
  2. Baulcombe D. 1994; Replicase-mediated resistance: a novel type of virus resistance in transgenic plants. Trends in Microbiology 2:60–63
    [Google Scholar]
  3. Carr J. P., Zaitlin M. 1993; Replicase-mediated resistance. Seminars in Virology 4:339–347
    [Google Scholar]
  4. Carr J. P., Gal-On A., Palukaitis P., Zaitlin M. 1994; Replicase-mediated resistance to cucumber mosaic virus in transgenic plants involves suppression of both virus replication in the inoculated leaves and long distance movement. Virology 199:439–447
    [Google Scholar]
  5. Ding S. -H., Anderson B. J., Haase H. R., Symons R. H. 1994; New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601
    [Google Scholar]
  6. Dreher T., Hall T. C. 1988; Mutational analysis of the sequence and structural requirements in brome mosaic virus RNA for minus strand promoter activity. Journal of Molecular Biology 201:31–40
    [Google Scholar]
  7. Golemboski D. B., Lomonossoff G. P., Zaitlin M. 1990; Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus. Proceedings of the National Academy of Sciences, U.S.A 87:6311–6315
    [Google Scholar]
  8. Habili N., Symons R. H. 1989; Evolutionary relationships between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Research 17:9543–9555
    [Google Scholar]
  9. Hase S., Karasawa A., Takahashi H., Ehara Y. 1992; Nucleotide sequence of RNA 2 of cucumber mosaic virus strain O. Annals of the Phytopathological Society of Japan 58:789–793
    [Google Scholar]
  10. Joshi R. L., Joshi S., Chapeville F., Haenni A. -L. 1983; tRNA-like structures of plant viral RNAs: conformational requirements for adenylation and aminoacylation. EMBO Journal 2:1123–1127
    [Google Scholar]
  11. Kataoka J., Masuta C., Takanami Y. 1990; Complete nucleotide sequence of RNA 2 of cucumber mosaic virus Y strain. Annals of the Phytopathological Society of Japan 56:495–500
    [Google Scholar]
  12. Longstaff M., Brigneti G., Boccard F., Chapman S., Baulcombe D. 1993; Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase. EMBO Journal 12:379–386
    [Google Scholar]
  13. MacFarlane S. A., Davies J. W. 1992; Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA 1 are resistant to virus infection. Proceedings of the National Academy of Sciences, U.S.A 89:5829–5833
    [Google Scholar]
  14. Marsh L. E., Pogue G. P., Hall T. C. 1989; Similarities among plant virus (+) and (−) RNA termini imply a common ancestry with promoters of eukaryotic tRNAs. Virology 172:415–427
    [Google Scholar]
  15. Mierendorf R. C., Pfeffer D. 1987; Direct sequencing of denatured plasmid DNA. Methods in Enzymology 152:556–562
    [Google Scholar]
  16. Morch M. -D., Boyer J. -C., Haenni A. -L. 1988; Overlapping open reading frames revealed by complete nucleotide sequencing of turnip yellow mosaic virus genomic RNA. Nucleic Acids Research 16:6157–6173
    [Google Scholar]
  17. Owen J., Palukaitis P. 1988; Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping in RNA 3 of eight CMV strains. Virology 166:495–502
    [Google Scholar]
  18. Owen J., Shintaku M., Aeschleman P., Ben Tahar S., Palukaitis P. 1990; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 3. Journal of General Virology 71:2243–2249
    [Google Scholar]
  19. Palukaitis P., Roossinck M. J., Dietzgen R. G., Francki R. I. B. 1992; Cucumber mosaic virus. Advances in Virus Research 41:281–348
    [Google Scholar]
  20. Pogue G. P., Hall T. C. 1992; The requirement for a 5′ stem-loop structure in brome mosaic virus replication supports a new model for viral positive-strand RNA initiation. Journal of Virology 66:674–684
    [Google Scholar]
  21. Pogue G. P., Marsh L. E., Connell J. P., Hall T. C. 1992; Requirement for ICR-like sequences in the replication of brome mosaic virus genomic RNA. Virology 188:742–753
    [Google Scholar]
  22. Rizzo T. M., Palukaitis P. 1988; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 2. Journal of General Virology 69:1777–1787
    [Google Scholar]
  23. Rizzo T. M., Palukaitis P. 1990; Construction of full-length cDNA clones of cucumber mosaic virus RNAs 1,2 and 3: generation of infectious RNA transcripts. Molecular and General Genetics 222:249–256
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  26. Tien P., Rao A. L. N., Hatta T. 1982; Cucumber mosaic virus from cornflower in China. Plant Disease 66:337–339
    [Google Scholar]
  27. Traynor P., Young B. M., Ahlquist P. 1991; Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread. Journal of Virology 65:2807–2815
    [Google Scholar]
  28. Zaitlin M., Anderson J. M., Perry K. L., Zhang L., Palukaitis P. 1994; Specificity of replicase-mediated resistance to cucumber mosaic virus. Virology 201: in press
    [Google Scholar]
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