1887

Abstract

The IgA antibody response to respiratory syncytial virus (RSV) was determined in nasopharyngeal secretions (NPS) of 22 infants and children infected with RSV group A strains, employing an ELISA. The antibody activity observed during the convalescent phase against whole virus, fusion glycoprotein (F) and large glycoprotein (G) was examined in young infants (under 6 months) and compared with that of older individuals (6 to 16 months). Both groups showed similar degrees of IgA antibody activity to whole virus in NPS; however, older individuals showed a significantly higher activity of IgA F antibody than that of IgA G antibody in the NPS. On the other hand, in the NPS of young infants, IgA F antibody was somewhat suppressed and IgA G antibody activity predominated over that of IgA F. Pre-existing (maternal) serum IgG anti-RSV F antibody activity was higher than that of antibody to G. A significant reverse correlation was observed between the activity of preexisting serum IgG F antibody and NPS IgA F antibody in the convalescent phase after primary infection with RSV. These observations suggest that maternally derived RSV IgG antibody, which contains abundant anti-F activity, may suppress the development of IgA F antibody response at infection sites in the respiratory tract in young infants during primary RSV infection. These changes may be related to the severity of acute infection and longer convalescence often observed in young infants during RSV infection.

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1994-08-01
2024-04-23
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References

  1. Belshe R. B., Anderson E. L., Walsh E. L. 1993; Immuno- genicity of purified F glycoprotein of respiratory syncytial virus: clinical and immune responses to subsequent natural infection in children. Journal of Infectious Diseases 168:1024–1029
    [Google Scholar]
  2. Hemming V. G., Lodriguez W., Kim H. W. 1987; Intravenous immunoglobulin treatment of respiratory syncytial virus infection in infants and young children. Antimicrobial Agents and Chemotherapy 31:1882–1886
    [Google Scholar]
  3. Johnson P. R., Collins P. L. 1988; The fusion glycoproteins of human respiratory syncytical virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  4. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987; The G glycoprotein of human respiratory syncytial viruses of subgroup A and B: extensive sequence divergence between anti- genically related proteins. Proceedings of the National Academy of Sciences, U.S.A 84:5625–5629
    [Google Scholar]
  5. Kaul T. N., Welliver R. C., Wong D. T., Udwadia R. A., Riddlesberger K., Ogra P. L. 1981; Secretory antibody response to respiratory syncytial virus infection. American Journal of Diseases of Children 135:1013–1016
    [Google Scholar]
  6. Kim H. W., Arrobio J. O., Brandt C. D., Jeffries B. C., Pyles G., Reid J. L., Chanock R. M., Parrot R. H. 1973; Epidemiology of respiratory syncytial virus infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. American Journal of Epidemiology 98:216–225
    [Google Scholar]
  7. McIntosh K., Chanock R. M. 1987; Respiratory syncytial virus. In Virology pp. 1285–1304 Fields B. N. Edited by New York: Raven Press;
    [Google Scholar]
  8. McIntosh K., Masters H. B., Orr I., Chao R. K., Barkin R. M. 1978; The immunologic response to infection with respiratory syncytial virus in infants. Journal of Infectious Diseases 138:24–32
    [Google Scholar]
  9. Murphy B. R., Alling D. W., Snyder M. H., Walsh E. E., Prince G. A., Chanock R. M., Hemming V. G., Rodriguez W. L., Kim H. W., Graham B. S., Wright P. F. 1986a; Effect of age and pre-existing antibody on serum antibody response of infants and childrent to the F and G glycoproteins during respiratory syncytial virus infection. Journal of Clinical Microbiology 24:894–898
    [Google Scholar]
  10. Murphy B. R., Graham B. S., Prince G. A., Walsh E. E., Chanock R. M., Karzon D. T., Wright P. F. 1986b; Serum and nasal-wash immunoglobulin G and A antibody response of infants and children to respiratory syncytial virus F and G glycoproteins following primary infection. Journal of Clinical Microbiology 23:1009–1014
    [Google Scholar]
  11. Nadal D., Ogra P. L. 1990; Development of local immunity: role in mechanism of protection against or pathogenesis of respiratory syncytial viral infections. Lung (supplement)379–387
    [Google Scholar]
  12. Nagai K., Tsutsumi H., Yamazaki H., Pattamadilok S., Chiba S. 1993; Three antigenic variant groups in human respiratory syncytial virus subgroup B isolated in Japan. Archives of Virology 128:55–63
    [Google Scholar]
  13. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Chanock R. M., Collins P. L. 1986; Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia: comparison of the individual contributions of the F and G glycoproteins to host immunity. Proceedings of the National Academy of Sciences, U.S.A 83:7462–7466
    [Google Scholar]
  14. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. 1973; Epidemiology of respiratory syncytial virus infection in Washington, D.C. 2. Infection and disease with respect to age, immunologic status, race and sex. American Journal of Epidemiology 98:289–300
    [Google Scholar]
  15. Tsutsumi H., Nagai K., Suga K., Chiba Y., Chiba S., Tsugawa S., Ogra P. L. 1989; Antigenic variation of human RSV strains isolated in Japan. Journal of Medical Virology 27:124–130
    [Google Scholar]
  16. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1985; Purification and characterization of the respiratory syncytial virus fusion protein. Journal of General Virology 66:409–415
    [Google Scholar]
  17. Walsh E. E., Cote P. J., Fernie B. F., Schlesinger J. J., Brandriss M. W. 1986; Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies. Journal of General Virology 67:505–513
    [Google Scholar]
  18. Welliver R. C., Sun M., Hildreth S. W., Arumugham R. A., Ogra P. L. 1989; Respiratory syncytial virus-specific antibody responses in immunoglobulin A and E isotypes to the F and G proteins and to intact virus after natural infection. Journal of Clinical Microbiology 27:295–299
    [Google Scholar]
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