@article{mbs:/content/journal/jgv/10.1099/0022-1317-75-8-2089, author = "Bristow, Richard G. W. and Douglas, Alan R. and Skehel, John J. and Daniels, Rod S.", title = "Analysis of murine antibody responses to baculovirus-expressed human immunodeficiency virus type 1 envelope glycoproteins", journal= "Journal of General Virology", year = "1994", volume = "75", number = "8", pages = "2089-2095", doi = "https://doi.org/10.1099/0022-1317-75-8-2089", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-8-2089", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "An analysis of the humoral immunogenicity of a candidate AIDS vaccine (VaxSyn) in a murine model system is presented. Sera taken from a panel of mice immunized with the immunogen were analysed for their ability to bind a panel of gpl20-representing peptides and limited reactivity to known sites of immunological interest was observed. Monoclonal antibodies (MAbs) were characterized as binding to a more restricted variety of regions on gpl20 including Cl, V2, V4 and the C terminus. A tetrazolium-based cytocidicity assay was used and shown to be an effective and objective method for the screening of human immunodeficiency virus (HlV)-neutralizing activity in large numbers of samples. None of the MAbs characterized in this study neutralize HIV-1 reference strains. The significance of these findings in view of previous publications is discussed.", }