1887

Abstract

The sequence of the fibre of bovine adenovirus type 3 (BAd3) predicts an extremely long structure due to a large number of 15-residue repeats in the fibre shaft, the tail and head domains being similar in size to the human adenovirus fibres. The length of the fibre was confirmed using negative-strain electron microscopy of BAd3 pentons (fibre plus penton base). The fibre was found to be bent in several discrete places and the bending sites appear to correspond with irregular repeats in the shaft. We suggest that bending of the fibre is needed for the interaction of the penton base with the secondary receptors on the cell surface.

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1994-08-01
2021-09-24
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References

  1. Devaux C., Adrian M., Berthet-Colominas C., Cusack S., Jacrot B. 1990; Structure of adenovirus fibre.I. Analysis of crystals of fibre from adenovirus serotypes 2 and 5 by electron microscopy and X-ray crystallography. Journal of Molecular Biology 215:567–588
    [Google Scholar]
  2. Gelderblom H., Maichle-Lauppe I. 1982; The fibers of fowl adenoviruses. Archives of Virology 72:289–298
    [Google Scholar]
  3. Graham F. L., Prevec L. 1991; Manipulation of adenovirus vectors. In Methods in Molecular Biology 7 Gene Transfer and Expression Techniques pp. 109–128 Murray E. J., Walker J. M. Edited by Clifton: Humana Press;
    [Google Scholar]
  4. Green N. M., Wrigley N. G., Russell W. C., Martin S. R., Mclachlan A. D. 1983; Evidence for a repeating cross-β sheet structure in the adenovirus fibre. EMBO Journal 2:1357–1365
    [Google Scholar]
  5. Gruber W. C., Russell D. J., Tibbetts C. 1993; Fiber gene and genomic origin of human adenovirus type 4. Virology 196:630–611
    [Google Scholar]
  6. Kidd A. H., Chroboczek J., Cusack S., Ruigrok R. W. H. 1993; Adenovirus type 40 virions contain two distinct fibres. Virology 192:73–84
    [Google Scholar]
  7. Laver W. G., Banfield-Younghusband H., Wrigley N. G. 1971; Purification and properties of chick embryo lethal orphan virus (an avian adenovirus). Virology 45:598–614
    [Google Scholar]
  8. Mittal S. K., Prevec L., Babiuk L. A., Graham F. L. 1992; Sequence analysis of bovine adenovirus type 3 early region 3 and fibre protein genes. Journal of General Virology 73:3295–3300
    [Google Scholar]
  9. Mittal S. K., Prevec L., Babiuk L. A., Graham F. L. 1993; Corrigendum.Sequence analysis of bovine adenovirus type 3 early region 3 and fibre protein genes. Journal of General Virology 74:2825
    [Google Scholar]
  10. Petterson U., Wadell G. 1985; Antigenic structure of the adenoviruses. In Immunochemistry of Viruses. The Basis for Sero-diagnosis and Vaccines pp. 295–323 van Regenmortel M. H. V., Neurath A. R. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  11. Ruigrok R. W. H., Barge A., Albiges-Rizo C., Dayan S. 1990; Structure of adenovirus fibre. II. Morphology of single fibres. Journal of Molecular Biology 215:589–596
    [Google Scholar]
  12. Stouten P. F. W., Sander C., Ruigrok R. W. H., Cusack S. 1992; A new triple-helical model for the shaft of the adenovirus fibre. Journal of Molecular Biology 226:1073–1084
    [Google Scholar]
  13. Wickham T. J., Mathias P., Cheresh D. A., Nemerow G. R. 1993; Integrins αvβ3 and Avβ5 promote adenovirus internalization but not virus attachment. Cell 73:309–319
    [Google Scholar]
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