1887

Abstract

The C-polyhedrin genes of two different geographic isolates of a type 5 cytoplasmic polyhedrosis virus (CPV) were cloned. A CPV infecting (OpCPV), isolated in the Pacific Northwest of the U.S.A., and a CPV infecting , isolated in South Africa, were studied. Both genes were found to be 883 nucleotides in length and encoded a predicted protein of 246 residues ( of 28890). Comparison of the nucleotide sequences of these two viruses with another type 5 geographic isolate, infecting (EsCPV; isolated in Eastern Canada), showed that there were only 17 nucleotide differences among the three genes. The only nucleotide variation that had an effect on the encoded protein was a deletion of nucleotide 774 in the gene of EsCPV. The deletion introduces a frameshift mutation resulting in the alteration of the carboxyl-terminal amino acid sequence. Sequence alignment of the OpCPV C-polyhedrin showed little homology to a type 1 CPV (infecting ) or with analogous proteins (N-polyhedrins) from two baculoviruses infecting . Interestingly, most of the conserved residues between the N- and C-polyhedrins were either basic or aromatic amino acids.

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/content/journal/jgv/10.1099/0022-1317-75-8-1969
1994-08-01
2019-11-19
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-8-1969
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