1887

Abstract

The C-polyhedrin genes of two different geographic isolates of a type 5 cytoplasmic polyhedrosis virus (CPV) were cloned. A CPV infecting (OpCPV), isolated in the Pacific Northwest of the U.S.A., and a CPV infecting , isolated in South Africa, were studied. Both genes were found to be 883 nucleotides in length and encoded a predicted protein of 246 residues ( of 28890). Comparison of the nucleotide sequences of these two viruses with another type 5 geographic isolate, infecting (EsCPV; isolated in Eastern Canada), showed that there were only 17 nucleotide differences among the three genes. The only nucleotide variation that had an effect on the encoded protein was a deletion of nucleotide 774 in the gene of EsCPV. The deletion introduces a frameshift mutation resulting in the alteration of the carboxyl-terminal amino acid sequence. Sequence alignment of the OpCPV C-polyhedrin showed little homology to a type 1 CPV (infecting or with analogous proteins (N-polyhedrins) from two baculoviruses infecting . Interestingly, most of the conserved residues between the N- and C-polyhedrins were either basic or aromatic amino acids.

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1994-08-01
2024-12-10
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References

  1. Arella M., Lavallee C., Belloncik S., Furuichi Y. 1988; Molecular cloning and characterization of cytoplasmic polyhedrosis virus polyhedrin and a viable deletion mutant gene. Journal of Virology 62:211–217
    [Google Scholar]
  2. Arif B. M. 1984; The entomopoxviruses. Advances in Virus Research 29:195–213
    [Google Scholar]
  3. Banville M., Dumas F., Trifiro S., Arif B., Richardson C. 1992; The predicted amino acid sequence of the spheroidin protein from Amsacta moorei entomopoxvirus: lack of homology between major occlusion body proteins of different poxviruses. Journal of General Virology 73:559–566
    [Google Scholar]
  4. Belloncik S. 1989; Cytoplasmic polyhedrosis viruses -Reoviridae. Advances in Virus Research 36:173–209
    [Google Scholar]
  5. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  6. Brown F. 1989; The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Edmonton, Canada 1987. Intervirology 30:181–186
    [Google Scholar]
  7. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  8. Fossiez F., Belloncik S., Arella M. 1989; Nucleotide sequence of the polyhedrin gene of Euxoa scandens cytoplasmic polyhedrosis virus (EsCPV). Virology 169:462–465
    [Google Scholar]
  9. Galinski M. S., Chow K.-C., Rohrmann G. F., Pearson G. D., Beaudreau G. S. 1982; Size determination of Orgyia pseudotsugata cytoplasmic polyhedrosis virus genomic RNA. Virology 123:328–335
    [Google Scholar]
  10. Galinski M. S., Stanik V. H., Rohrmann G. F., Beaudreau G. S. 1983; Comparison of sequence diversity in several cytoplasmic polyhedrosis viruses. Virology 130:372–380
    [Google Scholar]
  11. Galinski M. S., Mink M. A., Lambert D. M., Wechsler S. L., Pons M. W. 1986; Molecular cloning and sequence analysis of the human parainfluenza 3 virus RNA encoding the nucleocapsid protein. Virology 149:139–151
    [Google Scholar]
  12. Galinski M. S., Mink M. A., Pons M. W. 1988; Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein. Virology 165:499–510
    [Google Scholar]
  13. Galinski M. S., Troy R. M., Banerjee A. K. 1992; RNA editing in the phosphoprotein gene of the human parainfluenza virus type 3. Virology 186:543–550
    [Google Scholar]
  14. Grancher-Barray S., Boisvert J., Belloncik S. 1981; Electrophoretic characterization of proteins and RNA of cytoplasmic polyhedrosis virus (CPV) from Euxoa scandens. Archives of Virology 70:55–61
    [Google Scholar]
  15. Hopp T. P., Woods K. R. 1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of Sciences, U.S.A 78:3824–3828
    [Google Scholar]
  16. Konarska M. M., Padgett R. A., Sharp P. A. 1984; Recognition of cap structure in splicing in vitro of mRNA. Cell 38:731–736
    [Google Scholar]
  17. Maizel J. V.Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. In Methods in Virology pp. 179–246 Maramoroschand K., Koprowski H. Edited by New York: Academic Press;
    [Google Scholar]
  18. Martignoni M. C., Iwai P. J., Hughes K. M., Addison R. B. 1969; A cytoplasmic polyhedrosis of Hemerocampa pseudotsugata. Journal of Invertebrate Pathology 13:15–18
    [Google Scholar]
  19. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  20. Mertens P. P. C., Crook N. E., Rubinstein R., Pedley S., Payne C. C. 1989; Cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis. Journal of General Virology 70:173–185
    [Google Scholar]
  21. Payne C. C., Mertens P. P. C. 1983; Cytoplasmic polyhedrosis viruses. In The Reoviridae pp. 425–505 Joklik W. K. Edited by New York: Academic Press;
    [Google Scholar]
  22. Payne C. C., Rivers C. F. 1976; A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. Journal of General Virology 33:71–85
    [Google Scholar]
  23. Payne C. C., Piasecka-Serafin M., Pilley B. 1977; The properties of two recent isolates of cytoplasmic polyhedrosis viruses. Intervirology 8:155–163
    [Google Scholar]
  24. Payne C. C., Mertens P. P. C., Katagiri K. 1978; A comparitive study of three closely related cytoplasmic polyhedrosis viruses. Journal of Invertebrate Pathology 32:310–318
    [Google Scholar]
  25. Quiot J. M., Belloncik S. 1977; Characterization d’une polyed-roses cytoplasmique chez le lepidoptere Euxoa acandens [Noctuidae, Agrotinae], Etudes in vivo et in vitro. Archives of Virology 55:145–153
    [Google Scholar]
  26. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  27. Rubinstein R., Harley E. H., Losman M., Lutton D. 1976; The nucleic acids of viruses infecting Heliothis armigera. Virology 69:323–326
    [Google Scholar]
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