1887

Abstract

Human T cell leukaemia virus type I (HTLV-I) is a direct activator of human resting T lymphocytes. The present study was undertaken to delineate further the role of viral particles and to define the involvement of envelope glycoproteins in the induction of T cell mitogenic stimulation. Virus-producing cells treated with paraformaldehyde (PFA) were found to be unable to induce the formation of syncytia, but still able to trigger the proliferation of resting T cells. Likewise, PFA-treated virus particles were still mitogenic. These results suggest that the mitogenic event is triggered before the fusion of the envelope with the cell membrane. Furthermore, HTLV-I envelope-expressing cells obtained after infection of C8166/45 cells (HTLV-I- transformed, but defective in virion production) with an HTLV-I envelope recombinant vaccinia virus were unable to activate normal T cells. Human immunodeficiency virus type 1 particles produced by C8166/45 cells were also devoid of mitogenic ability. However, when HTLV-I viral preparations were purified by chromatography, only the virion-containing fractions were found to be mitogenic for human resting T lymphocytes. This mitogenic activity was partially abolished by preincubating the purified virus with a monoclonal antibody directed to the surface envelope glycoprotein. Finally, treatment of HTLV-I-transformed cells by tunicamycin, an inhibitor of -linked glyco-sylation, led to the production of virus particles with a decreased mitogenic activity. Collectively, these observations suggest that the HTLV-I mitogenic activity is triggered by the contact of HTLV-I virions with T cells.

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1994-08-01
2022-05-19
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References

  1. Arthur L. O., Bess J. W., Sowder R. C.II Benveniste R. E., Mann D. L., Chermann J. C., Enderson L. E. 1992; Cellular proteins bound to immunodeficiency viruses: implications for pathogenesis and vaccines. Science 258:1935–1938
    [Google Scholar]
  2. Cann A. J., Chen I. S. Y. 1990; Human T-cell leukemia virus types I and II. In Virology, 2nd edn. pp. 1501–1527 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  3. Chejanovsky N., Zakai N., Amselem S. Y. B., Loyter A. 1986; Membrane vesicles containing the Sendai virus binding glycoprotein, but not the viral fusion protein fuse with phosphatidylserine liposomes at low pH. Biochemistry 25:4810–4817
    [Google Scholar]
  4. Duc Dodon M., Gazzolo L. 1987; Loss of interleukin 2 requirement for the generation of T colonies defines an early event of human T-lymphotropic virus type I (HTLV-I) infection. Blood 69:12–17
    [Google Scholar]
  5. Duc Dodon M., Bernard A., Gazzolo L. 1989; Peripheral T- lymphocyte activation by human T-cell leukemia virus type I interferes with the CD2 but not with the CD3/TCR pathway. Journal of Virology 63:5413–5419
    [Google Scholar]
  6. Gaulton G. N., Greene M. I. 1989; Inhibition of cellular DNA synthesis by reovirus occurs through a receptor-linked signaling pathway that is mimicked by antiidiotypic, antireceptor antibody. Journal of Experimental Medicine 169:197–211
    [Google Scholar]
  7. Gazzolo L., Duc Dodon M. 1987; Human T-lymphotropic virus type 1 is a direct activator of resting T lymphocytes. Nature; London: 326:714–717
    [Google Scholar]
  8. Gebhardt A., Bosch J. V., Ziemiecki A., Friis F. R. R. 1984; Rous sarcoma virus p 19 and p35 can be chemically cross-linked to high molecular weight complexes - an insight into virus assembly. Journal of Molecular Biology 174:297–317
    [Google Scholar]
  9. Gilbert J. M., Mason D., White J. M. 1990; Fusion of Rous sarcoma virus with host cells does not require exposure to low pH. Journal of Virology 64:5106–5113
    [Google Scholar]
  10. Kimata J. T., Palker T. J., Ratner L. 1993; The mitogenic activity of human T-cell leukemia virus type I is T-cell associated and requires the CD2/LFA-3 activation pathway. Journal of Virology 67:3134–3141
    [Google Scholar]
  11. Lentz T. L. 1990; The recognition event between virus and host cell receptor: a target for antiviral agents. Journal of General Virology 71:751–766
    [Google Scholar]
  12. McGrath M., Witte O., Pincus T., Weissman I. L. 1978; Retrovirus purification: a method that conserves envelope glycoprotein and maximizes infectivity. Journal of Virology 25:923–927
    [Google Scholar]
  13. Matsushita S., Robert-Guroff M., Trepel J., Cossman J., Mitsuya H., Broder S. 1986; Human monoclonal antibody directed against an envelope glycoprotein of human T-cell leukemia virus type I. Proceedings of the National Academy of Sciences, U.S.A 83:2672–2676
    [Google Scholar]
  14. Palker T. J., Riggs E. R., Spragion D. E., Muir A. J., Scearce R. M., Randall R. R., McAdams M. W., McKnight A., Clapham P. R., Weiss R. A., Haynes B. F. 1992; Mapping of homologous, amino-terminal neutralizing regions of human T cell leukemia virus type 1 and II gp46 envelope glycoproteins. Journal of Virology 66:5879–5889
    [Google Scholar]
  15. Paulson J. C. 1985; Interactions of animal viruses with cell surface receptors. In The Receptors 2 pp. 131–219 Conn P. M. Edited by Orlando: Academic Press;
    [Google Scholar]
  16. Pique C., Pham D., Tursz T., Dokhálar M. -C. 1992; Human T- cell leukemia virus type I envelope protein maturation process: requirements for syncytium formation. Journal of Virology 66:906–913
    [Google Scholar]
  17. Popovic M., Lange-Wantzin G., Sarin P. S., Mann D., Gallo R. C. 1983; Transformation of human umbilical cord blood T-cells by human T-cell leukemia/lymphoma virus. Proceedings of the National Academy of Sciences, U.S.A 80:5402–5406
    [Google Scholar]
  18. Rey M. A., Spire B., Dormont D., Barre-Sinoussi F., Montagnier L., Chermann J. C. 1984; Characterization of the RNA dependent DNA polymerase of a new human T lymphotropic retrovirus (lymphoadenopathy associated virus). Biochemical and Biophysical Research Communications 121:126–133
    [Google Scholar]
  19. Salahuddin S. Z., Markham P. D., Wong-Staal F., Franchini G., Kalyaraman V. S., Gallo R. C. 1983; Restricted expression of human T-cell leukemia-lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes. Virology 129:51–64
    [Google Scholar]
  20. Satake M., Luftig R. 1983; Comparative immunofluorescence of murine leukemia virus-derived membrane associated antigens. Virology 124:259–273
    [Google Scholar]
  21. Shida H., Stochikura T., Sato T., Konno T., Hirayoshi K., Seki M., Ito Y., Hatanaka M., Hinuma Y., Sugimoto M., Takahashi-Nishimaki F., Maruyama T., Miki K., Suzuki K., Morita M., Sashiyama H., Hayami M. 1987; Effect of the recombinant vaccinia viruses that express HTLV-I envelope gene on HTLV-I infection. EMBO Journal 6:3379–3384
    [Google Scholar]
  22. Vallé A., Garrone P., Yssel H., Bonnefoy J. Y., Freedman A. S., Freeman G., Nadler L. M., Banchereau J. 1990; Mab 104, a new monoclonal antibody, recognizes the B7 antigen which is expressed on activated B cells and HTLV-I transformed T cells. Immunology 69:351–357
    [Google Scholar]
  23. Wucherpfennig K. W., Höllsberg P., Richardson J. H., Benjamin D. 1992; T-cell activation by autologous human T-cell leukemia virus type I-infected T-cell clones. Proceedings of the National Academy of Sciences, U.S.A 89:2110–2114
    [Google Scholar]
  24. Yu X., Yuan Z., Matsuda Z., Lee T. H., Essex M. 1992; The matrix protein of human immunodeficiency virus type I is required for incorporation of viral envelope protein into mature virions. Journal of Virology 66:4966–4971
    [Google Scholar]
  25. Zack J. A., Cann A. J., Lugo J. P., Chen I. S. Y. 1988; AIDS virus production from infected peripheral blood T cells following HTLV-I-induced mitogenic stimulation. Science 240:1026–1040
    [Google Scholar]
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