1887

Abstract

Electrophoretic profiles of the dsRNAs of field isolates of rice dwarf virus (RDV) were compared with those of an isolate maintained at Hokkaido University (RDV-H). Unexpectedly, the genomic dsRNAs of most of the field isolates showed distinct electrophoretic mobility profiles. This was the case even among isolates from the same region. Genome segment 12 (SI2) from some variants migrated faster than S12 from RDV-H. These RNAs were converted to full-length cDNAs and sequenced. S12 from all the variants had the same length of 1066 nucleotides with nucleotide sequence identities of 96 to 99%. Three open reading frames previously reported were present in all the variants, and the sequence identities were 95 to 99% for P12, 98 to 100% for P120Pa, and nearly 100% for P120Pb. A comparison of the nucleotide and amino acid sequences of the variants with sequences of the RDV-H and Akita isolate showed that there are two genomic types, one represented by RDV-H and the other by the Akita isolate.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-8-1843
1994-08-01
2022-05-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/8/JV0750081843.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-8-1843&mimeType=html&fmt=ahah

References

  1. Anzola J. V., Xu Z., Asamizu T., Nuss D. L. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, U.S.A 84:8301–8305
    [Google Scholar]
  2. Boccardo G., Milne R. G. 1984; Plant reovirus group. CMI/AAB Descriptions of Plant Viruses294
    [Google Scholar]
  3. Diaz-Ruiz J. R., Kaper J. M. 1978; Isolation of viral doublestranded RNAs using a LiCl fractionation procedure. Preparative Biochemistry 8:1–17
    [Google Scholar]
  4. Dulieu P., Bar-Joseph M. 1989; Rapid isolation of double stranded RNA segments from disulphide crosslinked polyacrylamide gels. Journal of Virological Methods 24:77–84
    [Google Scholar]
  5. Estes M. K., Choen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  6. Fukumoto F., Omura T., Minobe Y. 1989; Nucleotide sequence of segment S9 of the rice dwarf virus genome. Archives of Virology 107:135–139
    [Google Scholar]
  7. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  8. Hayashi N., Minobe Y. 1990; Segment 5 of the rice dwarf virus genome encodes a protein highly conserved within the phyto- reoviruses. Journal of General Virology 71:3081–3083
    [Google Scholar]
  9. Hillman B. J., Anzola J. V., Halpern B. T., Cavileer T. D., Nuss D. L. 1991; First field isolation of wound tumor virus from a plant host: minimal sequence divergence from the type strain isolated from an insect vector. Virology 185:896–900
    [Google Scholar]
  10. Kano H., Koizumi M., Noda H., Minobe H., Tsukihara T., Ishikawa K., Hibino H., Omura T. 1990; Nucleotide sequence of rice dwarf virus (RDV) genome segment S3 coding for 114K major core protein. Nucleic Acids Research 18:6700
    [Google Scholar]
  11. Kimura I., Minobe Y., Omura T. 1987; Changes in a nucleic acid and a protein component of rice dwarf virus particles associated with an increase in symptom severity. Journal of General Virology 68:3211–3215
    [Google Scholar]
  12. Kudo H., Uyeda I., Shikata E. 1991; Viruses in Phytoreovirus genus of the Reoviridae family have the same conserved terminal sequences. Journal of General Virology 72:2857–2866
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  14. Omura T., Minobe Y., Tsuchizaki T. 1988; Nucleotide sequence of segment S10 of the rice dwarf virus genome. Journal of General Virology 69:227–231
    [Google Scholar]
  15. Ramig R. F., Ward R. L. 1991; Genomic segment reassortment in rotavirus and other Reoviridae. Advances in Virus Research 39:163–207
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-termination inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  18. Suzuki N., Watanabe Y., Kusano T., Kitagawa Y. 1990a; Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5 and S6: comparison with the equivalent wound tumor virus segments. Virology 179:446–454
    [Google Scholar]
  19. Suzuki N., Watanabe Y., Kusano T., Kitagawa Y. 1990b; Sequence analysis of the rice dwarf phytoreovirus segment S3 transcript encoding for the major structural core protein of 114 kDa. Virology 179:455–459
    [Google Scholar]
  20. Suzuki N., Tanimura M., Watanabe Y., Kusano T., Kitagawa Y., Suda N., Kudo H., Uyeda I., Shikata E. 1992a; Molecular analysis of rice dwarf phytoreovirus segment SI: interviral homology of the putative RNA-dependent RNA polymerase between plant- and animal-infecting reoviruses. Virology 190:240–247
    [Google Scholar]
  21. Suzuki N., Sugawara M., Kusano T. 1992b; Rice dwarf phytoreovirus segment S12 transcript is tricistronic in vitro . Virology 191:992–995
    [Google Scholar]
  22. Uyeda I., Shikata E. 1984; Characterization of RNAs synthesized by the virion-associated transcription of rice dwarf virus in vitro . Virus Research 1:527–532
    [Google Scholar]
  23. Uyeda I., Matsumura T., Sano T., Ohshima K., Shikata E. 1987; Nucleotide sequence of rice dwarf virus genome segment 10. Proceedings of the Japan Academy Series B 63:227–230
    [Google Scholar]
  24. Uyeda I., Kudo H., Takahashi T., Sano T., Ohshima K., Matsumura T., Shikata E. 1989; Nucleotide sequence of rice dwarf virus genome segment 9. Journal of General Virology 70:1297–1300
    [Google Scholar]
  25. Uyeda I., Suda N., Yamada N., Kudo H., Murao K., Suga H., Kimura I., Shikata E., Kitagawa Y., Kusano T., Sugawara M., Suzuki N. 1993; Nucleotide sequence of rice dwarf phytoreovirus genome segment 2: completion of sequence analyses of rice dwarf virus. Intervirology in press
    [Google Scholar]
  26. Yamada N., Kudo H., Uyeda I., Shikata E. 1990; Nucleotide sequence of rice dwarf virus genome segment 3. Nucleic Acids Research 18:6419
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-8-1843
Loading
/content/journal/jgv/10.1099/0022-1317-75-8-1843
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error