@article{mbs:/content/journal/jgv/10.1099/0022-1317-75-7-1807, author = "Raynes, Deborah A. and Hartshorne, David J. and Guerriero, Vince", title = "Sequence and expression of a baculovirus protein with antigenic similarity to telokin", journal= "Journal of General Virology", year = "1994", volume = "75", number = "7", pages = "1807-1809", doi = "https://doi.org/10.1099/0022-1317-75-7-1807", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-7-1807", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its M r on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of β structure. A cDNA for TLP20 was isolated and sequenced. The 3′ non-coding sequence contained a region of high identity with the 5′ end of two other baculovirus genes. The 5′ non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower M r than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.", }