1887

Abstract

Chicken peripheral blood lymphocytes (PBLs) show morphological and biochemical features of apoptosis (programmed cell death) when infected with infectious bursal disease virus (IBDV). DNA extracted from IBDV-infected lymphocytes displayed an intense laddering pattern when visualized after agarose gel electrophoresis. IBDV-infected PBLs had significantly higher apoptotic and necrotic indices measured by acridine orange-ethidium bromide staining than did control lymphocytes. Electron micrographs of the IBDV-infected PBLs revealed features typical of apoptosis such as peripheral condensation of chromatin, blebbing of the plasma membrane and fragmentation of the nucleus and of the cell leading to the formation of apoptotic bodies. These findings indicate that IBDV, in addition to causing necrosis, can also induce apoptosis in avian lymphocytes

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-7-1803
1994-07-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/7/JV0750071803.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-7-1803&mimeType=html&fmt=ahah

References

  1. Ameisen J. C., Capron A. 1991; Cell dysfunction and depletion in AIDS: the programmed cell death hypothesis. Immunology Today 12:102–105
    [Google Scholar]
  2. Asakawa J., Tsiagbe V. K., Thorbecke G. J. 1993; Protection against apoptosis in chicken bursa cells by phorbol ester in vitro. Cellular Immunology 147:180–187
    [Google Scholar]
  3. Burkhardt E., Müller H. 1987; Susceptibility of chicken blood lymphoblasts and monocytes to infectious bursal disease virus (IBDV). Archives of Virology 94:297–303
    [Google Scholar]
  4. Cohen J. J. 1991; Programmed cell death in the immune system. Advances in Immunology 50:55–85
    [Google Scholar]
  5. Duke R. C., Cohen J. J. 1992; Morphological and biochemical assays of apoptosis. In Current Protocols in Immunology 1 pp. 3.17.1–3.17.16 Coligan J., Kruisbeek A. M., Argulies D. H., Evach E. M., Strober W. Edited by New York: John Wiley & Sons;
    [Google Scholar]
  6. Galvan M., Lau S., Somasundarum T., Concepcion R., Ahmed R. 1993; T cell activation, apoptosis, and memory in viral infection. 12th Annual Meeting, American Society for Virology Davis, California: Abstract 46-2 p. A105
    [Google Scholar]
  7. Groux H., Monte D., Bourrez J. M., Capron A., Ameisen J. C. 1991; Activation of CD4+ T-lymphocytes in asymptomatic HIV infected patients induce the program action of lymphocyte death by apoptosis. Comptes Rendus de l’Academie des Sciences, Serie III, Sciences de la Vie 312:599–606
    [Google Scholar]
  8. Hugin A. W., Vacchio M. S., Morse H. C. 1991; A virus encoded ‘superantigen’ in a retrovirus-induced immunodeficiency syndrome of mice. Science 252:424–427
    [Google Scholar]
  9. Jeurissen S. H. M., Wagenaar F., Pol J. M. A., van der Eb A. J., Noteborn M. H. M. 1992; Chicken anemia virus causes apoptosis of thymocytes after in vivo infection and of cell lines after in vitro infection. Journal of Virology 66:7383–7388
    [Google Scholar]
  10. Lam K. M. 1988; Lysis of chicken lymphocytes by infectious bursal disease viruses. Avian Diseases 32:818–821
    [Google Scholar]
  11. Lukert P. D., Saif Y. M. 1991; Infectious bursal disease. In Diseases of Poultry, 9th edn. pp. 648–663 Calnek B. W., Barnes H. J., Beard C. W., Reid W. M., Yoder H. W. Jr Edited by Ames: Iowa State University Press;
    [Google Scholar]
  12. Mccabe M. J., Orrenius S. 1992; Deletion and depletion: the involvement of viruses and environmental factors in T-lymphocyte apoptosis. Laboratory Investigation 66:403–406
    [Google Scholar]
  13. Meyaard L., Otto S. A., Jonker R. R., Munster M. J., Keet R. P. M., Miedema F. 1992; Programmed death of T cells in HIV-1 infection. Science 257:217–219
    [Google Scholar]
  14. Motyka B., Reynolds J. D. 1991; Apoptosis is associated with the extensive B cell death in the sheep ilealPeyer’s patch and the chicken bursa of Fabricius: a possible role in B cell selection. European Journal of Immunology 21:1951–1958
    [Google Scholar]
  15. Müller H. 1986; Replication of infectious bursal disease virus in lymphoid cells. Archives of Virology 87:191–203
    [Google Scholar]
  16. Razvi E. S., Welsh R. M. 1993; Programmed cell death (apoptosis) of T lymphocytes during acute viral infection: a mechanism for virus-induced immune deficiency. 12th Annual Meeting, American Society for Virology Davis, California: Abstract 46-3 p. A106
    [Google Scholar]
  17. Rojko J. L., Fulton R. M., Renanza L. J., Williams L. L., Copelan E., Cheney C. M., Reichel G. S., Neil J. C., Mathes L. E., Fisher T. G., Cloyd M. W. 1992; Lymphocytotoxic strains of feline leukemia virus induce apoptosis in feline T4-thymic lymphoma cells. Laboratory Investigation 66:418–426
    [Google Scholar]
  18. Rosenberger J. K., Cloud S. S., Gelb J., Odor E., Dohms J. E. 1985; Sentinel bird survey of Delmarva broiler flocks. Proceedings of the 20th National Meeting of Poultry Health and Condemnations Ocean City, Maryland: pp. 194–202
    [Google Scholar]
  19. Sharma J. M., Dohms J. E., Metz A. L. 1989; Comparative pathogenesis of serotype 1 and variant serotype 1 isolates of infectious bursal disease virus and the effect of those viruses on humoral and cellular immune response of specific-pathogen-free chickens. Avian Diseases 33:112–124
    [Google Scholar]
  20. Snyder D. B. 1990; Changes in the field status of infectious bursal disease virus. Avian Pathology 19:419–423
    [Google Scholar]
  21. Wyllie A. H. 1981; Cell death: a new classification separating apoptosis from necrosis. In Cell Death in Biology and Pathology pp. 9–34 Bowen I. D., Lockshin R. A. Edited by London: Chapman & Hall;
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-75-7-1803
Loading
/content/journal/jgv/10.1099/0022-1317-75-7-1803
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error