1887

Abstract

Rotavirus strains belonging to G types 1 to 4 and having a P3 genotype (M3 7-like VP4) were recovered from children with symptomatic and asymptomatic infections. Partial sequences of their VP4 genes were determined in an attempt to characterize these strains further. The genomic regions encoding VP8*, the connecting and putative fusion peptides and three other regions in VP5* were sequenced. The deduced amino acid sequences were compared with rotavirus strains belonging to different P genotypes that had been previously reported. High degrees of identity were found between the VP8* fragment of all human P3 strains (92·7 to 99·7%) suggesting that they belong to the same genotype, regardless of differences in their virulence. Furthermore, based on comparative sequence analysis, we did not identify any amino acid(s) that differ appreciably between symptomatic and asymptomatic strains and could therefore be associated with virulence. The results suggest that the P3 genotype, although frequently associated with asymptomatic infections, may not be the single determining factor in attenuation of symptoms.

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1994-07-01
2022-01-21
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References

  1. Bern C., Unicomb L., Gentsch J. R., Banul N., Yunus M., Sack R. B., Glass R. I. 1992; Rotavirus diarrhea in Bangladeshi children: correlation of disease severity with serotypes. Journal of Clinical Microbiology 30:3234–3238
    [Google Scholar]
  2. Bishop R. F., Barnes G. L., Cipriani E., Lund J. S. 1983; Clinical immunity after neonatal rotavirus infection - A prospective longitudinal study in young children. New England Journal of Medicine 14:72–76
    [Google Scholar]
  3. Chrystie I. L., Totterdill B. M., Banatvala J. E. 1978; Asymptomatic endemic rotavirus infections in the newborn. Lancet i:1176–1178
    [Google Scholar]
  4. Coulson B. 1993; Typing of human rotavirus VP4 by an enzyme immunoassay using monoclonal antibodies. Journal of Clinical Microbiology 31:1–8
    [Google Scholar]
  5. Das M., Dunn S. J., Woode G. N., Greenberg H. B., Rao C. D. 1993; Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (1321) have high levels of sequence identity with homologous proteins of a serotype 10 bovine rotavirus. Virology 194:374–379
    [Google Scholar]
  6. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  7. Flores J., Midthun K., Hoshino Y., Green K., Gorziglia M., Kapikian A. Z., Chanock R. M. 1986; Conservation of the fourth gene among rotavirus recovered from asymptomatic newborn infants and its possible role in attenuation. Journal of Virology 60:972–979
    [Google Scholar]
  8. Flores J., Perez-Schael I., Blanco M., White L., Garcia D., Vilar M., Cunto W., Gonzalez R., Urbina C., Boher J., Mendez M., Kapikian A. Z. 1990; Comparison of reactogenicity and antigenicity of M37 rotavirus vaccine and rhesus-rotavirus-based quadrivalent vaccine. Lancet 336:336–340
    [Google Scholar]
  9. Gentsch J. R., Glass R. I., Woods P., Gouvea V., Gorziglia M., Flores J., Das B. K., Bhan M. K. 1992; Identification of group A rotavirus gene 4 types by polymerase chain reaction. Journal of Clinical Microbiology 30:1365–1373
    [Google Scholar]
  10. Gentsch J. R., Das B. K., Jiang B., Bhan M. K., Glass R. I. 1993; Similarity of the VP4 protein of human rotavirus strain 116E to that of the bovine B223 strain. Virology 194:424–430
    [Google Scholar]
  11. Gorziglia M., Green K., Nishikawa K., Taniguchi K., Jones R., Kapikian A. Z., Chanock R. M. 1988; Sequence of the fourth gene of human rotavirus recovered from asymptomatic or symptomatic infections. Journal of Virology 62:2978–2984
    [Google Scholar]
  12. Gorziglia M., Nishikawa K., Hoshino Y., Taniguchi K. 1990; Similarity of the outer capsid protein VP4 of the Gottfried strain of porcine rotavirus to that of asymptomatic human rotavirus strains. Journal of Virology 64:414–418
    [Google Scholar]
  13. Hafpejee I. E. 1991; Neonatal rotavirus infections. Reviews of Infectious Diseases 13:957–962
    [Google Scholar]
  14. Hoshino Y., Wyatt R. G., Flores J., Midthun K., Kapikian A. Z. 1985; Serotype characterization of rotavirus derived from asymptomatic human neonatal infections. Journal of Clinical Microbiology 21:425–430
    [Google Scholar]
  15. Kapikian A. Z., Chanock R. M. 1990; Rotaviruses. In Virology pp. 1353–1403 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  16. Kobayashi N., Taniguchi K., Urasawa T., Urasawa S. 1991; Preparation and characterization of a neutralizing monoclonal antibody directed to VP4 of rotavirus strain K8 which has unique VP4 neutralization epitopes. Archives of Virology 121:153–162
    [Google Scholar]
  17. Larralde G., Flores J. 1990; Identification of gene 4 alleles among human rotaviruses by polymerase chain reaction-derived probes. Virology 179:469–473
    [Google Scholar]
  18. Li B., Clark H. F., Gouvea V. 1993; Nucleotide sequence of the VP4 encoding gene of an unusual human rotavirus (HCR3). Virology 196:825–830
    [Google Scholar]
  19. Midthun K., Halsey N. A., Jett-Goheen M., Clements M. L., Steinhoff M., King J. C., Karron R., Wilson M., Burns B., Perkins V., Samorodin R., Kapikian A. Z. 1991; Safety and immunogenicity of human rotavirus vaccine M37 in adults, children, and infants. Journal of Infectious Diseases 164:792–796
    [Google Scholar]
  20. Perez-Schael I., Daoud G., White L., Urbina G., Daoud N., Perez M., Flores J. 1984; Rotavirus shedding by newborn children. Journal of Medical Virology 14:127–136
    [Google Scholar]
  21. Qian Y., Green K. Y. 1991; Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology 182:407–412
    [Google Scholar]
  22. Rodger S. M., Bishop R. F., Birch C., Mclean B., Holmes I. H. 1981; Molecular epidemiology of human rotavirus in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13:272–278
    [Google Scholar]
  23. Santos N., Gouvea V. 1994; Improved method for purification of viral RNA from fecal specimens for rotavirus detection. Journal of Virological Methods 46:11–21
    [Google Scholar]
  24. Santos N., Riepenhoff-Talty M., Clark H. F., Offit P., Gouvea V. 1994; VP4 genotyping of human rotavirus in the United States. Journal of Clinical Microbiology 32:205–208
    [Google Scholar]
  25. Steele A. D., Garcia D., Sears J., Gerna G., Nakagomi O., Flores J. 1993a; Distribution of VP4 alleles in human rotavirus by using probes to the hyperdivergent region of the VP4 gene. Journal of Clinical Microbiology 31:1735–1740
    [Google Scholar]
  26. Steele A. D., Mnisi Y. N., Bos W. P., Aspinall S. 1993b; Electrophoretic typing of nosocomial rotavirus infection in a general paediatric unit showing the continual introduction of community strains. Journal of Medical Virology 40:126–132
    [Google Scholar]
  27. Taniguchi K., Nishikawa K., Urasawa T., Urasawa S., Midthun K., Kapikian A. Z., Gorziglia M. 1989; Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. Journal of Virology 63:4101–4106
    [Google Scholar]
  28. Taniguchi K., Urasawa T., Kobayashi N., Gorziglia M., Urasawa S. 1990; Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. Journal of Virology 64:5640–5644
    [Google Scholar]
  29. Vesikari T., Ruuska T., Koivu H., Green K., Flores J., Kapikian A. Z. 1991; Evaluation of the M37 human rotavirus vaccine in 2- to 6-month-old infants. Pediatric Infectious Disease Journal 10:912–917
    [Google Scholar]
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