1887

Journal of General Virology header logo

Volume 75, Issue 7

Identification of novel transcripts complementary to the Marek's disease virus homologue of the ICP4 gene of herpes simplex virus Free

Abstract

Libraries of cDNA were generated from polyadenylated RNAs derived from Marek’s disease virus (MDV)- transformed cell lines by directional cloning of oligo- (dT)-primed cDNAs in lambda gt22A. Analysis of the libraries for viral sequences showed that a number of cDNA clones originated from transcripts mapping in the A region of the MDV genome. Sequencing and line mapping of these cDNAs suggested that the RNA transcripts expressed from this region were either in the sense or antisense direction with respect to the MDV homologue of the ICP4 gene of herpes simplex virus. The longest cDNA clone from antisense transcripts was 2756 bp long and partially overlapped the 5′ end of the coding region of the ICP4 gene. The cDNA clone contained at least four introns, shown by comparison of its sequence with the sequence of the ICP4 gene. The presence of introns was confirmed by PCR analysis. All the introns have the consensus splice donor and acceptor signals at their 5′ and 3′ ends respectively. Northern blot analysis showed that the ICP4 gene homologue of MDV was abundantly transcribed only in lytically infected fibroblasts, whereas transcripts complementary to the ICP4 gene were the major transcripts in MDV- transformed cell lines and lymphomas. The transcripts complementary to ICP4 consist of two major RNA species approximately 15 kb and 1·32 kb long. The results suggest that there might be an inverse relationship between the abundance of ICP4 transcripts and their complementary transcripts in MDV-infected and transformed cells.

  • Received:
  • Accepted:
  • Published Online:
© The Journal of General Virology 1994
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-7-1713
1994-07-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/7/JV0750071713.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-7-1713&mimeType=html&fmt=ahah

References

  1. Akiyama Y., Kato S. 1974; Two cell lines from lymphomas of Marek’s disease. Biken Journal 17:105–116
     [Google Scholar]
  2. Anderson A. S., Francesconi A., Morgan R. 1992; Complete nucleotide sequence of the Marek’s disease virus ICP4 gene. Virology 189:657–667
     [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidmain J. G., Smith J. A., Struhl K. 1989 Short Protocols in Molecular Biology New York: John Wiley;
     [Google Scholar]
  4. Brooks L. A., Lear A. L., Young L. S., Rickinson A. B. 1993; Transcripts from the Epstein-Barr virus BamHI A fragment are detectable in all three forms of virus latency. Journal of Virology 67:3182–3190
     [Google Scholar]
  5. Buckmaster A. E., Scott S. D., Sanderson M. J., Boursnell M. E. G., Ross N. L. J., Binns M. M. 1988; Gene sequence and mapping data from Marek’s disease virus and herpesvirus of turkeys: implications for herpesvirus classification. Journal of General Virology 69:2033–2042
     [Google Scholar]
  6. Calnek B. W., Schat K. A. 1991; Proliferation of chicken lymphoblastoid cells after in vitro infection with Marek’s disease virus. Avian Diseases 35:728–737
     [Google Scholar]
  7. Calnek B. W., Shek W. R., Schat K. A. 1981; Spontaneous and induced herpesvirusgenome expression in Marek’s disease tumour cell lines. Infection and Immunity 34:483–491
     [Google Scholar]
  8. Cebrian J., Dierich C. K., Berthelot N., Sheldrick P. 1982; Inverted repeat nucleotide sequences in the genomes of Marek’s disease virus and the herpesvirus of the turkey. Proceedings of the National Academy of Sciences, U.S.A 79:555–558
     [Google Scholar]
  9. Chen X., Velicer L. F. 1991; Multiple bidirectional initiations and terminations of transcription in the Marek’s disease virus long repeat regions. Journal of Virology 65:2445–2451
     [Google Scholar]
  10. Delecluse H.-J., Schuller S., Hammerschmidt W. 1993; Latent Marek’s disease virus can be activated from its chromosomally integrated state in herpesvirus-transformed lymphoma cells. EMBO Journal 12:3277–3286
     [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
     [Google Scholar]
  12. Faber S. W., Wilcox K. W. 1986; Association of the herpes simplex virus regulatory protein ICP4 with specific nucleotide sequence in DNA. Nucleic Acids Research 14:6067–6083
     [Google Scholar]
  13. Fukuchi K., Sudo M., Lee Y.-S., Tanaka A., Nonoyama M. 1984a; Structure of Marek’s disease virus DNA; detailed restriction enzyme map. Journal of Virology 51:102–109
     [Google Scholar]
  14. Fukuchi K., Tanaka A., Nonoyama M. 1984b; The structure of Marek’s disease virus DNA. In The International Symposium on Marek’s Disease pp. 68–83 Calnek B. W., Spencer J. L. Edited by Ithaca: Cornell University Press;
     [Google Scholar]
  15. Gilligan K. G., Sato H., Rajadurai P., Busson P., Young L., Rickinson A., Tursz T., Raab-Traub N. 1990; Novel transcription from the Epstein-Barr virus terminal EcoRI fragment, DIJhet, in a nasopharyngeal carcinoma. Journal of Virology 64:4948–4956
     [Google Scholar]
  16. Goridon Y. J., Johnson B., Romanowski E., Araullo-Cruz T. 1988; RNA complementary to herpes simplex virus type 1 ICP0 gene demonstrated in neurons of human trigeminal ganglia. Journal of Virology 62:1832–1835
     [Google Scholar]
  17. Hirai K., Maotani K., Ikuta K., Yasue H., Ishibashi M., Kato S. 1986; Chromosomal sites for Marek’s disease virus DNA in two chicken lymphoblastoid cell lines MDCC-MSB1 and MDCC-RP1. Virology 152:256–261
     [Google Scholar]
  18. Hitt M. M., Allday M. J., Hara T., Karran L., Jones M. D., Busson P., Tursz T., Ernberg I., Griffin B. E. 1989; EBV gene expression in an NPC-related tumour. EMBO Journal 8:2639–2651
     [Google Scholar]
  19. Kanamori A., Ikuta K., Ueda S., Kato S., Hirai K. 1987; Methylation of Marek’s disease virus DNA in chicken T-lymphoblastoid cell lines. Journal of General Virology 68:1485–1490
     [Google Scholar]
  20. Karran L., Gao Y., Smith P. R., Griffin B. E. 1992; Expression of a family of complementary-strand transcripts in Epstein-Barr virus-infected cells. Proceedings of the National Academy of Sciences, U.S.A 89:8058–8062
     [Google Scholar]
  21. Mount S. M. 1982; A catalogue of splice junction sequences. Nucleic Acids Research 10:459–472
     [Google Scholar]
  22. Nakajima K., Ikuta K., Naito M., Ueda S., Kato S., Hirai K. 1987; Analysis of Marek’s disease virus serotype 1-specific phosphorylated polypeptides in virus-infected cells and Marek’s disease lymphoblastoid cells. Journal of General Virology 68:1379–1389
     [Google Scholar]
  23. Nazerian K., Stephens E. A., Sharma J. M., Lee L. F., Gailitis M., Witter R. L. 1977; A non-producer T lymphoblastoid cell line from Marek’s disease transplantable tumour (JMV). Avian Diseases 21:69–76
     [Google Scholar]
  24. Priola S. A., Stevens J. G. 1991; The 5′ and 3′ limits of transcription in the pseudorabies virus latency associated transcription unit. Virology 182:852–856
     [Google Scholar]
  25. Priola S. A., Gustafson D. P., Wagner E. K., Stevens J. G. 1990; A major portion of the latent pseudorabies virus genome is transcribed in trigeminal ganglia of pigs. Journal of Virology 64:4755–4760
     [Google Scholar]
  26. Rock D. L., Nesburn A. B., Ghiasi H., Ong J., Lewis T. L., Lokensgard J. R., Wechsler S. L. 1987; Detection of latency-related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1. Journal of Virology 61:3820–3826
     [Google Scholar]
  27. Ross L. J. N., Basarab O., Walker D. J., Whitby B. 1975; Serological relationship between a pathogenic strain of Marek’s disease virus, its attenuated derivative and herpes virus of turkeys. Journal of General Virology 28:37–47
     [Google Scholar]
  28. Ross L. J. N., Sanderson M., Scott S. D., Binns M. M., Doel T., Milne B. 1989; Nucleotide sequence and characterization of the Marek’s disease virus homologue of glycoprotein B of herpes simplex virus. Journal of General Virology 70:1789–1804
     [Google Scholar]
  29. Ross N. L. J., Delorbe W., Varmus H. E., Bishop J. M., Brahic M., Haase A. 1981; Persistence and expression of Marek’s disease virus DNA in tumour cells and peripheral nerves studied by in situ hybridization. Journal of General Virology 57:285–296
     [Google Scholar]
  30. Rziha H.-J., Bauer B. 1982; Circular forms of viral DNA in Marek’s disease virus-transformed lymphoblastoid cells. Archives of Virology 72:211–216
     [Google Scholar]
  31. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  32. Santos M. A. 1991; An improved method for the small scale preparation of bacteriophage DNA based on phage precipitation by zinc chloride. Nucleic Acids Research 19:5442
     [Google Scholar]
  33. Schat K. A., Buckmaster A., Ross L. J. N. 1989; Partial transcription map of Marek’s disease herpesvirus in lyrically infected cells and lymphoblastoid cell lines. International Journal of Cancer 44:101–109
     [Google Scholar]
  34. Silver S., Tanaka A., Nonoyama M. 1979; Transcription of the Marek’s disease virus genome in a nonproductive chicken lymphoblastoid cell line. Virology 93:127–133
     [Google Scholar]
  35. Smith P. R., Gao Y., Karran L., Jones M. D., Snudden D., Griffin B. E. 1993; Complex nature of the major viral polyadenylated transcripts in Epstein-Barr virus-associated tumors. Journal of Virology 67:3217–3225
     [Google Scholar]
  36. Spivack J. G., Woods G. M., Fraser N. W. 1991; Identification of a novel latency-specific splice donor signal within the herpes simplex virus type-1 2·0-kilobase latency-associated transcript (LAT): translation inhibition of LAT open reading frames by the intron within the 2·0-kilobase LAT. Journal of Virology 65:6800–6810
     [Google Scholar]
  37. Stevens J. G., Wagner E. K., Devi-Rao G. B., Cook M. L., Feldman L. T. 1987; RNA complementary to a herpesvirus a gene mRNA is prominent in latently infected neurons. Science 28:1056–1059
     [Google Scholar]
  38. Sugaya K., Bradley G., Nonoyama M., Tanaka A. 1990; Latent transcripts of Marek’s disease virus are clustered in the short and long repeat regions. Journal of Virology 64:5773–5782
     [Google Scholar]
  39. Velicer L. F., Brunovskis P. 1992; Genomic organization of herpesviruses in Marek’s disease system. Proceedings of the XIX World Poultry Congress 1:33–39
     [Google Scholar]
  40. Wagner E. K., Flanagan W. M., Devi-Rao G., Zhang Y., Hill J. J., Anderson K. P. A., Stevens J. G. 1988; The herpes simplex virus latency-associated transcript is spliced during the latent phase of infection. Journal of Virology 62:4577–4585
     [Google Scholar]
  41. Zhang C. X., Lowrey P., Finerty S., Morgan A. J. 1993; Analysis of Epstein-Barr virus gene transcription in lymphoma induced by the virus in the cottontoptamarin by construction of a cDNA library with RNA extracted from a tumour biopsy. Journal of General Virology 74:509–514
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-7-1713
Loading
Identification of novel transcripts complementary to the Marek's disease virus homologue of the ICP4 gene of herpes simplex virus
J. Gen. Virol. 75, 1713 (1994); https://doi.org/10.1099/0022-1317-75-7-1713
/content/journal/jgv/10.1099/0022-1317-75-7-1713
/content/journal/jgv/10.1099/0022-1317-75-7-1713
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error