1887

Abstract

Kinetic analysis of the two divergent immediate early (IE) transcription units of bovine herpesvirus 1 (BHV-1) revealed an unexpected behaviour. The IE1.7 promoter was not turned off at the end of the IE period but acted as a late promoter, unlike the adjacent IE4.2/2.9 promoter which was active only under IE conditions. The genome region specifying the IE 1.7 gene was sequenced (0·814 to 0·839 map units). The IE1.7 promoter was found to overlap with duplicated sequence elements bearing close similarity to herpesvirus origins of replication, which may explain the biphasic transcription kinetics. Exons 1 and 2 of the spliced IE1.7 transcript were non-coding. Exon 3 was found to contain a single open reading frame encoding a protein of 300 amino acids that was designated BICP22 because of its homology to ICP22 (Vmw68) of herpes simplex virus type 1 and related proteins from other herpesviruses. The protein probably represents IEP-55, the most abundant BHV-1 phosphoprotein observed under IE conditions.

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1994-07-01
2021-10-19
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References

  1. Ackermann M., Sarmiento M., Roizman B. 1985; Application of antibody to synthetic peptides for characterization of the intact and truncated a22 protein specified by herpes simplex virus 1 and the R325 a22- deletion mutant. Journal of Virology 56:207–215
    [Google Scholar]
  2. Bairoch A. 1992; PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Research 20:2013–2018
    [Google Scholar]
  3. Baumann R. P., Yalamanchili V. R. R., O’Callaghan D. J. 1989; Functional mapping and DNA sequence of an equine herpesvirus 1 origin of replication. Journal of Virology 63:1275–1283
    [Google Scholar]
  4. Blaho J. A., Mitchell C., Roizman B. 1993; Guanylylation and adenylylation of the alpha-regulatory proteins of herpes simplex virus require a viral-beta or viral-gamma function. Journal of Virology 67:3891–3900
    [Google Scholar]
  5. Camp H. S., Coussens P. M., Silva R. F. 1991; Cloning, sequencing, and functional analysis of a Marek’s disease virus origin of DNA replication. Journal of Virology 65:6320–6324
    [Google Scholar]
  6. Carpenter D. E., Misra V. 1992; Sequences of the bovine herpesvirus 1 homologue of herpes simplex virus type-1 alpha-trans-inducing factor (UL48). Gene 119:259–263
    [Google Scholar]
  7. Cullinane A. A., Rixon F. J., Davison A. J. 1988; Characterization of the genome of equine herpesvirus 1 subtype 2. Journal of General Virology 69:1575–1590
    [Google Scholar]
  8. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  9. Depamphilis M. L. 1993; Eukaryotic DNA replication - anatomy of an origin. Annual Review of Biochemistry 62:29–63
    [Google Scholar]
  10. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  11. Faisst S., Meyer S. 1992; Compilation of vertebrate-encoded transcription factors. Nucleic Acids Research 20:3–26
    [Google Scholar]
  12. Fraefel C., Wirth U. V., Vogt B., Schwyzer M. 1993; Immediate-early transcription over covalently joined genome ends of bovine herpesvirus 1: the circ gene. Journal of Virology 67:1328–1333
    [Google Scholar]
  13. Gray C. P., Kaerner H. C. 1984; Sequence of the putative origin of replication in the UL region of herpes simplex virus type 1 ANG DNA. Journal of General Virology 65:2109–2119
    [Google Scholar]
  14. Hayes M. K., Rock D. L. 1990; Identification of a novel bovine herpesvirus type 1 immediate-early infected cell protein. Archives of Virology 112:291–300
    [Google Scholar]
  15. Holden V. R., Yalamanchili R. R., Harty R. N., O’Callaghan D. J. 1992; ICP22 homolog of equine herpesvirus 1: expression from early and late promoters. Journal of Virology 66:664–673
    [Google Scholar]
  16. Jackers P., Defechereux P., Baudoux L., Lambert C., Massaer M., Merville-Louis M. P., Rentier B., Piette J. 1992; Characterization of regulatory functions of the varicella-zoster virus gene 63-encoded protein. Journal of Virology 66:3899–3903
    [Google Scholar]
  17. Klupp B. G., Kern H., Mettenleiter T. C. 1992; The virulencedetermining genomic BamHI fragment 4 of pseudorabies virus contains genes corresponding to the UL15 (partial), UL18, UL19, UL20, and UL21 genes of herpes simplex virus and a putative origin of replication. Virology 191:900–908
    [Google Scholar]
  18. Mccarty D. M., Ni T. H., Muzyczka N. 1992; Analysis of mutations in adeno-associated virus rep protein in vivo and in vitro. Journal of Virology 66:4050–4057
    [Google Scholar]
  19. Mcgeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  20. Mcgeoch D. J., Dolan A., Donald S., Brauer D. H. K. 1986; Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1. Nucleic Acids Research 14:1727–1745
    [Google Scholar]
  21. Mcgeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., Mcnab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  22. Martin D. W., Deb S. P., Klauer J. S., Deb S. 1991; Analysis of the herpes simplex virus type 1 OriS sequence: mapping of functional domains. Journal of Virology 65:4359–4369
    [Google Scholar]
  23. Misra V., Blumenthal R. M., Babiuk L. A. 1981; Proteins specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). Journal of Virology 40:367–378
    [Google Scholar]
  24. Olivo P. D., Nelson N. J., Challberg M. D. 1988; Herpes simplex virus DNA replication: the UL9 gene encodes an originbinding protein. Proceedings of the National Academy of Sciences, U.S.A 85:5414–5418
    [Google Scholar]
  25. Poffenberger K. L., Raichlen P. E., Herman R. C. 1993; In vitro characterization of a herpes simplex virus type-1 ICP22 deletion mutant. Virus Genes 7:171–186
    [Google Scholar]
  26. Purves F. C., Ogle W. O., Roizman B. 1993; Processing of the herpes simplex virus regulatory protein alpha-22 mediated by the U(L)13 protein kinase determines the accumulation of a subset of alpha-messenger RNAs and gamma-messenger RNAs and proteins in infected cells. Proceedings of the National Academy of Sciences, U.S.A 90:6701–6705
    [Google Scholar]
  27. Rogers S., Wells R., Rechsteiner M. 1986; Amino acid sequence common to rapidly degraded proteins: the PEST hypothesis. Science 234:364–368
    [Google Scholar]
  28. Sakaguchi M., Urakawa T., Hirayama Y., Miki N., Yamamoto M., Hirai K. 1993; Sequence determination and genetic content of an 8·9-kb restriction fragment in the short unique region and the internal inverted repeat of Marek’s disease virus type-1 DNA (correction of vol. 6, pp. 365-378, 1992). Virus Genes 7:U209
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  30. Schwyzer M. 1993; Genome map of bovine herpesvirus 1. In Genetic Maps, 6th edn. pp. 1.166–1.170 O’Brien S. J. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Schwyzer M., Vlcek C., Menekse O., Fraefel C., Paces V. 1993; Promoter, spliced leader, and coding sequence for BICP4, the largest of the immediate-early proteins of bovine herpesvirus 1. Virology 197:349–357
    [Google Scholar]
  32. Sears A. E., Halliburton I. W., Meignier B., Silver S., Roizman B. 1985; Herpes simplex virus 1 mutants deleted in the α22 gene: growth and gene expression in permissive and restrictive cells and establishment of latency in mice. Journal of Virology 55:338–348
    [Google Scholar]
  33. Stow N. D., Davison A. J. 1986; Identification of a varicella- zoster virus origin of DNA replication and its activation by herpes simplex virus type 1 gene products. Journal of General Virology 67:1613–1623
    [Google Scholar]
  34. Stow N. D., Mcmonagle E. C. 1983; Characterization of the TRS/IRS origin of DNA replication of herpes simplex virus type 1. Virology 130:427–438
    [Google Scholar]
  35. Sugden B., Warren N. 1989; A promoter of Epstein-Barr virus that can function during latent infection can be transactivated by EBNA-1, a viral protein required for viral DNA replication during latent infection. Journal of Virology 63:2644–2649
    [Google Scholar]
  36. Telford E. A., Watson M. S., Mcbride K. E., Davison A. J. 1992; The DNA sequence of equine herpesvirus-1. Virology 189:304–316
    [Google Scholar]
  37. Vlcek C., Kozmik Z., Paces V., Schirm S., Schwyzer M. 1990; Pseudorabies virus immediate-early gene overlaps with an oppositely oriented open reading frame: characterization of their promoter and enhancer regions. Virology 179:365–377
    [Google Scholar]
  38. Whitton J. L., Clements J. B. 1984; Replication origins and a sequence involved in coordinate induction of the immediate-early gene family are conserved in an intergenic region of herpes simplex virus. Nucleic Acids Research 12:2061–2079
    [Google Scholar]
  39. Wirth U. V., Gunkel K., Engels M., Schwyzer M. 1989; Spatial and temporal distribution of bovine herpesvirus 1 transcripts. Journal of Virology 63:4882–4889
    [Google Scholar]
  40. Wirth U. V., Vogt B., Schwyzer M. 1991; The three major immediate-early transcripts of bovine herpesvirus 1 arise from two divergent and spliced transcription units. Journal of Virology 65:195–205
    [Google Scholar]
  41. Wirth U. V., Fraefel C., Vogt B., Vlcek C., Paces V., Schwyzer M. 1992; Immediate early RNA 2.9 and early RNA 2.6 of bovine herpesvirus 1 are 3′ coterminal and encode a putative zinc finger transactivator protein. Journal of Virology 66:2763–2772
    [Google Scholar]
  42. Wong S. W., Schaffer P. A. 1991; Elements in the transcriptional regulatory region flanking herpes simplex virus type 1 oriS stimulate origin function. Journal of Virology 65:2601–2611
    [Google Scholar]
  43. Wyler R., Engels M., Schwyzer M. 1989; Infectious bovine rhinotracheitis/vulvovaginitis (BHV-1). In Herpesvirus Diseases of Cattle, Horses, and Pigs pp. 1–72 Wittmann G. Edited by Boston: Kluwer;
    [Google Scholar]
  44. Zhang G., Leader D. P. 1990; The structure of the pseudorabies virus genome at the end of the inverted repeat sequences proximal to the junction with the short unique region. Journal of General Virology 71:2433–2441
    [Google Scholar]
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