RT Journal Article SR Electronic(1) A1 Bonning, Bryony C. A1 Roelvink, Peter W. A1 Vlak, Just M. A1 Possee, Robert D. A1 Hammock, Bruce D.YR 1994 T1 Superior expression of juvenile hormone esterase and β-galactosidase from the basic protein promoter of Autographa californica nuclear polyhedrosis virus compared to the p10 protein and polyhedrin promoters JF Journal of General Virology, VO 75 IS 7 SP 1551 OP 1556 DO https://doi.org/10.1099/0022-1317-75-7-1551 PB Microbiology Society, SN 1465-2099, AB The expression characteristics of the p10, polyhedrin and basic protein promoters of Autographa califomica nuclear polyhedrosis virus were compared using two reporter enzymes, juvenile hormone esterase (JHE) and β-galactosidase. In these systems, JHE is exported from the cell and β-galactosidase is localized to the cytosol. Expression of JHE from the basic, p10 and polyhedrin promoters was first detected in the medium at 13,19 and 27 h post-infection respectively. The basic protein promoter yielded the highest expression of the three promoters tested for both enzymes, as determined by protein and enzyme activity assays. In addition, yields of β-galactosidase and JHE under control of the p10 promoter are higher relative to expression under control of the polyhedrin promoter. These data highlight the importance of investigation of viral promoters other than the polyhedrin promoter for high yield protein expression in vitro, and for insecticidal use of recombinant baculoviruses requiring high levels of expression. The results support revision of the current concept that very late viral promoters are always optimal for high yield recombinant protein expression., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-7-1551