1887

Abstract

The nucleotide sequence (9306 nucleotides) of cDNA clones of apple stem pitting virus (ASPV) obtained from a double-stranded RNA template, extracted from diseased plant tissue, was determined. The genome is composed of five open reading frames (ORFs) encoding putative proteins with s of 247083, 25147, 12832, 7429 and 43712, and has a poly(A) tail. Using two oligonucleotides designed from the ASPV sequence information a 1598 bp fragment from near the 3′ terminus of the viral RNA, containing the coat protein of 43 766, was amplified from vein yellows (VY)-infected pear plants by PCR. The sequence determined showed eight nucleotide changes resulting in five amino acid substitutions compared with the sequence of ASPV. When compared to potex-, carla-, clostero- and capillo- viruses, the ASPV genome organization appeared to be most closely related to that of potexviruses, but with a larger coat protein of 44K (ORF5). The predicted coat protein size was confirmed by immunoblot analysis. The results show that ASPV does not fall into subgroup A of the closteroviruses but that it probably belongs in an as yet undefined group of viruses. They also suggest that the virus associated with VY is a strain of ASPV.

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1994-07-01
2024-12-09
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References

  1. Argos P. 1988; A sequence motif in many polymerases. Nucleic Acids Research 16:9909–9916
    [Google Scholar]
  2. Beck D. L., Forster R. L. S., Bevan M. W., Boxen K. A., Lowe S. C. 1990; Infectious transcripts and nucleotide sequence of cloned cDNA of the potexvirus white clover mosaic virus. Virology 177:152–158
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  4. Cameron H. R. 1989; Pear vein yellows. In Virus and Viruslike Diseases of Pome Fruits and Simulating Noninfectious Disorders pp. 175–181 Friedlund P. R. Edited by Cooperative Extension College of Agriculture and Home Economics, Washington State University, Pullman, Washington, SP0003:
    [Google Scholar]
  5. Coffin R. S., Coutts R. H. A. 1993; The closteroviruses, capilloviruses and other similar viruses: a short review. Journal of General Virology 74:1475–1483
    [Google Scholar]
  6. Dawson W. O., Bubrick P., Grantham G. L. 1988; Modifications of the tobacco mosaic virus coat protein gene affecting replication, movement, and symptomatology. Phytopathology 78:783–789
    [Google Scholar]
  7. Deborde D. C., Naeve C. W., Herlocher M. L., Maassab H. F. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidetransferase. Analytical Biochemistry 157:275–282
    [Google Scholar]
  8. Devereaux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  9. German S., Candresse T., Lanneau M., Huet J. C., Pernollet J. C., Dunez J. 1990; Nucleotide sequence and genomic organization of apple chlorotic leaf spot closterovirus. Virology 179:104–112
    [Google Scholar]
  10. Giunchedi L., Poggi Pollini C. 1992; Cytopathological, negative staining and serological electron microscopy of a clostero-like virus associated with pear vein yellows disease. Journal of Phytopathology 134:329–335
    [Google Scholar]
  11. Gramstat A., Courtpozanis A., Rohde W. 1990; The 12kDA protein of potato virus M displays properties of a nucleic acidbinding regulatory protein. FEBS Letters 276:34–38
    [Google Scholar]
  12. Habili N., Symons H. 1989; Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Research 17:9543–9555
    [Google Scholar]
  13. Jelkmann W., Martin R. R., Maiss E. 1989; Cloning of four plant viruses from small quantities of double-stranded RNA. Phytopathology 79:1250–1253
    [Google Scholar]
  14. Jelkmann W., Kunze L., Vetten H.-J., Lesemann D.-E. 1992a; cDNA cloning of dsRNA associated with apple stem pitting disease and evidence for the relationship of the virus-like agents associated with apple stem pitting and pear vein yellows. Actahorticulturae 309:55–62
    [Google Scholar]
  15. Jelkmann W., Maiss E., Martin R. R. 1992b; The nucleotide sequence and genome organization of strawberry mild yellow edge- associated potexvirus. Journal of General Virology 73:475–479
    [Google Scholar]
  16. Kanyuka K. V., Vishnichenko V. K., Levay K. E., Kondrikov D. Yu., Ryabov E. V., Zavriev S. K. 1992; Nucleotide sequence of shallot virus X RNA reveals a 5′-proximal cistron closely related to those of potexviruses and a unique arrangement of the 3′-proximal cistrons. Journal of General Virology 73:2553–2560
    [Google Scholar]
  17. Kegler H., Verderevskaja T., Fuchs E. 1979; Untersuchungen ueber Weehselbeziehungen verschiedener Kern- und Steinobstvirosen. Archiv für Gartenbau 27:325–336
    [Google Scholar]
  18. Koenig R. 1982; Carlavirus group. CMI/AAB Descriptions of Plant Viruses259
    [Google Scholar]
  19. Koenig R., Lesemann D.-E. 1978; Potexvirus group. CMI/AAB Descriptions of Plant Viruses200
    [Google Scholar]
  20. Koganezawa H., Yanase H. 1990; A new type of elongated virus isolated from apple trees containing the stem pitting agent. Plant Disease 74:610–614
    [Google Scholar]
  21. Kozak M. 1987; An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8148
    [Google Scholar]
  22. Marchuk D., Drumm M., Saulino A., Collins F. S. 1990; Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Research 19:1154
    [Google Scholar]
  23. Morozov S. YU., Kanyuka K. V., Levay K. E., Zavriev S. K. 1990; The putative RNA replicase of potato virus M: obvious sequence similarity with potex- and tymoviruses. Virology 179:911–914
    [Google Scholar]
  24. Morozov S. Yu., Miroshnichenko A. G., Solovyev D. A., Zelenina O. N., Fedorkin L. I., Lukasheva S. A., Grachev S. A., Chernov B. K. 1991; In vitro membrane binding of the translation products of the carlavirus 7-kDa protein genes. Virology 183:782–785
    [Google Scholar]
  25. Refatti E., Osler R. 1975; Possible relationship among pome fruit viruses detected in graft transmission trials. Acta horticulturae 44:201–208
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  28. Skryabin K. G., Kraev A. S., Morozov S. Yu., Rozanov M. N., Chernov B. K., Lukasheva L. I., Atabekov J. G. 1988; The nucleotide sequence of potato virus X RNA. Nucleic Acids Research 16:10929–10930
    [Google Scholar]
  29. Stouffer R. F. 1989; Apple stem pitting. In Virus and Viruslike Diseases of Pome Fruits and Simulating Noninfectious Disorders pp. 138–144 Friedlund P. R. Edited by Cooperative Extension College of Agriculture and Home Economics, Washington State University, Pullman, Washington, SP0003:
    [Google Scholar]
  30. Sumi S., Tsuneyoshi T., Furutani H. 1993; Novel rod-shaped viruses isolated from garlic, Allium sativum, possessing a unique genome organization. Journal of General Virology 74:1879–1885
    [Google Scholar]
  31. van Etten J. L., Freer S. N., Mccune B. K. 1979; Presence of a major (storage?) protein in dormant spores of the fungus Botryodiplodia theobromae. Journal of Bacteriology 138:650–652
    [Google Scholar]
  32. Yanase H., Koganezawa H., Friedlund P. R. 1989; Correlation of pear necrotic spot with pear vein yellows and apple stem pitting, and a flexuous filamentous virus associated with them. Acta horticulturae 235:157
    [Google Scholar]
  33. Yoshikawa N., Sasaki E., Kato M., Takahashi T. 1992; The nucleotide sequence of apple stem grooving capillovirus genome. Virology 191:98–105
    [Google Scholar]
  34. Zavriev S. K., Kanyuka K. V., Levay K. E. 1991; The genome organization of potato virus M RNA. Journal of General Virology 72:9–14
    [Google Scholar]
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