@article{mbs:/content/journal/jgv/10.1099/0022-1317-75-6-1511, author = "O’Rourke, K. I. and Huff, T. P. and Leathers, C. W. and Robinson, M. M. and Gorham, J. R.", title = "SCID mouse spleen does not support scrapie agent replication", journal= "Journal of General Virology", year = "1994", volume = "75", number = "6", pages = "1511-1514", doi = "https://doi.org/10.1099/0022-1317-75-6-1511", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-6-1511", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "BALB/e and severe combined immunodeficiency (SCID) mice were inoculated intracerebrally or intraperitoneally with scrapie agent strain ME7 to examine the role of functional lymphocytes and follicular dendritic cells in splenic infectivity and PrPSe accumulation. Intracerebrally inoculated BALB/c and SCID mice developed the clinical signs and microscopic lesions characteristic of scrapie. Spleens from terminally affected BALB/c mice contained PrPSc which was detectable by immuno- blot analysis; SCID mouse spleens did not contain detectable PrPSc. SCID mouse spleens collected during the first 90 days after intraperitoneal infection contained neither infectivity nor PrPSc.", }