1887

Abstract

BALB/e and severe combined immunodeficiency (SCID) mice were inoculated intracerebrally or intraperitoneally with scrapie agent strain ME7 to examine the role of functional lymphocytes and follicular dendritic cells in splenic infectivity and PrP accumulation. Intracerebrally inoculated BALB/c and SCID mice developed the clinical signs and microscopic lesions characteristic of scrapie. Spleens from terminally affected BALB/c mice contained PrP which was detectable by immuno- blot analysis; SCID mouse spleens did not contain detectable PrP. SCID mouse spleens collected during the first 90 days after intraperitoneal infection contained neither infectivity nor PrP.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-6-1511
1994-06-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/6/JV0750061511.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-6-1511&mimeType=html&fmt=ahah

References

  1. Basler K., Oesch B., Scott M., Westaway D., Walchli M., Groth D. F., Mckinley M. P., Prusiner S. B., Weissmann C. 1986; Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene. Cell 46:417–428
    [Google Scholar]
  2. Bendheim P. E., Brown H. R., Rudelli R. D., Scala L. J., Goller N. L., Wen G. Y., Kascsak R. J., Cashman N. R., Bolton D. C. 1992; Nearly ubiquitous tissue distribution of the scrapie agent precursor protein. Neurology 42:149–156
    [Google Scholar]
  3. Brown P., Gajdusek D. C. 1991; The human spongiform encephalopathies: Kuru, Creutzfeldt-Jakob disease, and the Gerstmann-Straussler-Scheinker syndrome. Current Topics in Microbiology and Immunology 172:1–20
    [Google Scholar]
  4. Caughey B., Race R. E., Chesebro B. 1988; Detection of prion protein mRNA in normal and scrapie-infected tissues and cell lines. Journal of General Virology 69:711–716
    [Google Scholar]
  5. Chandler R. L. 1969; Observations on the ultrastructure of the spinal cord, spleen and lymph nodes of mice inoculated with scrapie. Research in Veterinary Science 10:292–293
    [Google Scholar]
  6. Clarke M. C., Kimberlin R. H. 1984; Pathogenesis of mouse scrapie: distribution of agent in the pulp and stroma of infected spleens. Veterinary Microbiology 9:215–225
    [Google Scholar]
  7. Diringer H., Gelderblom H., Hilmert H., Ozel M., Edelbluth C., Kimberlin R. H. 1983; Scrapie infectivity, fibrils and low molecular weight protein. Nature; London: 306476–478
    [Google Scholar]
  8. Doi S., Ito M., Shinagawa M., Sato G., Isomura H., Goto H. 1988; Western blot detection of scrapie-associated fibril protein in tissues outside the central nervous system from preclinical scrapie- infected mice. Journal of General Virology 69:955–960
    [Google Scholar]
  9. Eklund C. M., Kennedy R. C., Hadlow W. J. 1967; Pathogenesis of scrapie virus infection in the mouse. Journal of Infectious Diseases 117:15–22
    [Google Scholar]
  10. Farquhar C. F., Somerville R. A., Ritchie L. A. 1989; Postmortem immunodiagnosis of scrapie and bovine spongiform encephalopathy. Journal of Virological Methods 24:215–222
    [Google Scholar]
  11. Hartsough G. R., Burger D. 1965; Encephalopathy of mink. 1. Epizootiologic and clinical observations. Journal of Infectious Diseases 115:387–392
    [Google Scholar]
  12. Ikegami Y., Ito M., Isomura H., Momotani E., Sasaki K., Muramatsu Y., Ishiguro N., Shinagawa M. 1991; Preclinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep. Veterinary Record 128:271–275
    [Google Scholar]
  13. Kapasi Z. F., Burton G. F., Schultz L. D., Tew J. G., Szakal A. K. 1993; Induction of functional follicular dendritic cell development in severe combined immunodeficiency mice: influence of B and T cells. Journal of Immunology 150:2648–2658
    [Google Scholar]
  14. Kitamoto T., Mohri S., Tateishi J. 1989; Organ distribution of proteinase-resistant prion protein in humans and mice with Creutzfeldt-Jakob disease. Journal of General Virology 70:3371–3379
    [Google Scholar]
  15. Kitamoto T., Muramoto T., Mohri S., Doh-Ura K., Tateishi J. 1991; Abnormal isoform of prion protein accumulates in follicular dendritic cells in mice with Creutzfeldt-Jakob disease. Journal of Virology 65:6292–6295
    [Google Scholar]
  16. Leggett M. M., Dukes J., Pirie H. M. 1990; A spongiform encephalopathy in a cat. Veterinary Record 127:586–588
    [Google Scholar]
  17. Mcbride P. A., Eickelenboom P., Kraal G., Fraser H., Bruce M. E. 1992; PrP protein is associated with follicular dendritic cells of spleens and lymph nodes in uninfected and scrapie-infected mice. Journal of Pathology 168:413–418
    [Google Scholar]
  18. Mcfarlin D. E., Raff M. C., Simpson E., Nehlsen S. H. 1971; Scrapie in immunologically deficient mice. Nature; London: 233336
    [Google Scholar]
  19. Mckinley M. P., Bolton D. C., Prusiner S. B. 1983; A protease- resistant protein is a structural component of the scrapie prion. Cell 35:57–62
    [Google Scholar]
  20. Merz P. A., Rohwer R. G., Kascsak R. J., Wisniewski H. M., Somerville R. A., Gibbs C. J., Gajdusek D. C. 1984; Infection- specific particle from the unconventional slow virus diseases. Science 224:437–440
    [Google Scholar]
  21. Merz P. A., Kascsak R. J., Rubenstein R., Carp R. I., Wisniewski H. M. 1987; Antisera to scrapie-associated fibril protein and prion protein decorate scrapie-associated fibrils. Journal of Virology 61:42–19
    [Google Scholar]
  22. Mori S., Wolfinbarger J. B., Miyazawa M., Bloom M. E. 1991; Replication of Aleutian mink disease parvovirus in lymphoid tissues of adult mink: involvement of follicular dendritic cells and macrophages. Journal of Virology 65:952–956
    [Google Scholar]
  23. Oesch B., Westaway D., Walchli M., Mckinley M. P., Kent S. B. H., Aebersold R., Barry R. A., Tempst P., Teplow D. B., Hood L. E., Prusiner S. B., Weissmann C. 1985; A cellular gene encodes scrapie PrP27-30 protein. Cell 40:735–746
    [Google Scholar]
  24. Onodera T., Ikeda T., Muramatsu Y., Shinagawa M. 1993; Isolation of scrapie agent from the placenta of sheep with natural scrapie in Japan. Microbiology and Immunology 37:311–316
    [Google Scholar]
  25. Prusiner S. B. 1982; Novel proteinaceous infectious particles cause scrapie. Science 216:136–144
    [Google Scholar]
  26. Race R. E., Ernst D. 1992; Detection of proteinase K-resistant prion protein and infectivity in mouse spleen by 2 weeks after scrapie agent inoculation. Journal of General Virology 73:3319–3323
    [Google Scholar]
  27. Race R., Ernst D., Jenny A., Taylor W., Sutton D., Caughey B. 1992; Diagnostic implications of detection of proteinase K- resistant protein in spleen, lymph nodes, and brain of sheep. American Journal of Veterinary Research 53:883–889
    [Google Scholar]
  28. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  29. Robinson M. M., Gorham J. R. 1990; Pathogenesis of hamster scrapie. Adherent splenocytes are associated with relatively high levels of infectivity. Archives of Virology 112:283–289
    [Google Scholar]
  30. Shinagawa M., Munekata E., Doi S., Takahashi K., Goto H., Sato G. 1986; Immunoreactivity of a synthetic pentadecapeptide corresponding to the N-terminal region of the scrapie prion protein. Journal of General Virology 67:1745–1750
    [Google Scholar]
  31. Tam J. P. 1988; Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system. Proceedings of the National Academy of Sciences U.S.A.: 85:5409–5413
    [Google Scholar]
  32. Tenner-Racz K., Racz P., Dietrich M., Kern P. 1985; Altered follicular dendritic cells and virus-like particles in AIDS and AIDS- related lymphadenopathy. Lancet i:105–106
    [Google Scholar]
  33. Wells G. A. H., Scott A. C., Johnson C. T., Gunning R. D., Hancock R. D., Jeffrey M., Dawson M., Bradley R. 1987; A novel progressive spongiform encephalopathy in cattle. Veterinary Record 121:419–420
    [Google Scholar]
  34. Wiley C. A., Burrola P. G., Buchmeier M. J., Wooddell M. K., Barry R. A., Prusiner S. B., Lampert P. W. 1987; Immuno- gold localization of prion filaments in scrapie-infected hamster brains. Laboratory Investigation 57:646–656
    [Google Scholar]
  35. Williams E. W., Young S. 1980; Chronic wasting disease of captive mule deer: a spongiform encephalopathy. Journal of Wildlife Diseases 16:89–98
    [Google Scholar]
  36. Worthington M., Clark R. 1971; Lack of effect of immunosuppression on scrapie infection in mice. Journal of General Virology 13:349–351
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-6-1511
Loading
/content/journal/jgv/10.1099/0022-1317-75-6-1511
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error