1887

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Volume 75, Issue 6

Characterization of the vaccinia virus L1R myristylprotein as a component of the intracellular virion envelope Free

Abstract

In many cases, virus-encoded acylproteins appear to localize to specific cellular and viral membranes and to be directly involved with the processes of virus morphogenesis and/or egress from the infected cell. It was therefore of interest to determine whether the major vaccinia virus (W) myristylprotein, L1R, is specifically associated with one or more of the membranes enveloping various infectious forms of W virions. To this end, single-membraned intracellular virions (INV) and extracellular enveloped virions (EEV), which are surrounded by at least two distinct membranes, were purified from W-infected cell lysates. The location of the VV L1R protein was determined by using a monospecific anti-L1R serum to detect the L1R protein by immunoblot in INV- and EEV-containing fractions, by examining the proteinase K sensitivity of the L1R protein in intact INV and EEV particles, and by immunoelectron microscopy. The data obtained clearly indicate that although the L1R protein is a constituent of both the INV and EEV particles, it is exclusively found in the inner INV-specific membrane. These results are discussed with regard to the potential role of the W LIR protein in the primary intracellular envelopment of infectious W particles.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1994
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1994-06-01
2024-04-20
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References

  1. Blasco R., Moss B. 1991; Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-dalton outer envelope antigen. Journal of Virology 65:5910–5920
     [Google Scholar]
  2. Blasco R., Moss B. 1992; Role of cell-associated enveloped vaccinia virus in cell-to-cell spread. Journal of Virology 66:4170–4179
     [Google Scholar]
  3. Child S. J., Hruby D. E. 1992; Evidence for multiple species of vaccinia virus-encoded palmitylated proteins. Virology 191:262–271
     [Google Scholar]
  4. Chow M., Newman J. F. E., Filman D., Hogle J. M., Rowlands D. J., Brown F. 1987; Capsid protein VP4 of picornavirus particles is covalently modified at its N-terminus by myristic acid. Nature; London: 327:482–486
     [Google Scholar]
  5. Dales S., Mosbach E. H. 1968; Vaccinia as a model for membrane biogenesis. Virology 35:564–583
     [Google Scholar]
  6. Dales S., Pogo B. G. 1981; Biology of poxviruses. Virology Monographs 18:54–60
     [Google Scholar]
  7. Dorns R. W., Lamb R. A., Rose J. K., Helenius A. 1993; Folding and assembly of viral membrane proteins. Virology 193:545–562
     [Google Scholar]
  8. Erickson P. A., Anderson D. H., Fisher S. K. 1987; Use of uranyl acetate en bloc to improve tissue preservation and labeling for post-embedding immunoelectron microscopy. Journal of Electron Microscopy Techniques 5:303–314
     [Google Scholar]
  9. Franke C. A., Reynolds P. R., Hruby D. E. 1989; Fatty acid acylation of vaccinia virus proteins. Journal of Virology 63:4285–4291
     [Google Scholar]
  10. Franke C. A., Wilson E. M., Hruby D. E. 1990; Use of a cell- free system to identify the vaccinia virus L1R gene product as the major late myristylated virion protein M25. Journal of Virology 64:5988–5996
     [Google Scholar]
  11. Goebel S., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
     [Google Scholar]
  12. Grand R. J. A. 1989; Acylation of viral and eukaryotic proteins. Biochemical Journal 258:625–638
     [Google Scholar]
  13. Hiller G., Weber K. 1985; Golgi-derived double membranes that contain an acylated viral polypeptide are used for vaccinia virus envelopment. Journal of Virology 55:651–659
     [Google Scholar]
  14. Lee P.-Y., Hruby D. E. 1993; trans-processing of vaccinia virus core proteins. Journal of Virology 67:4252–4263
     [Google Scholar]
  15. Marimoto T., Arpin M., Gaetani S. 1983; Use of proteases for the study of membrane insertion. Methods in Enzymology 96:121–150
     [Google Scholar]
  16. Payne L. G., Kristensson K. 1979; Mechanism of vaccinia virus release and its specific inhibition by N1-isonicotinoyl-N2-3-methyl-4-chlorobenzoylhydrazine. Journal of Virology 32:614–622
     [Google Scholar]
  17. Payne L. G., Kristensson K. 1982a; The effect of cytochalasin D and monensin on enveloped vaccinia virus release. Archives of Virology 74:11–20
     [Google Scholar]
  18. Payne L. G., Kristensson K. 1982b; Effect of glycosylation inhibitors on the release of enveloped vaccinia virus. Journal of Virology 41:367–375
     [Google Scholar]
  19. Persing D. H., Varmus H. E., Ganem D. 1987; The preSl protein of hepatitis B virus is acylated at its amino terminus with myristic acid. Journal of Virology 61:1672–1677
     [Google Scholar]
  20. Ravanello M. P., Franke C. A., Hruby D. E. 1993; An NH2-terminal peptide from the vaccinia virus L1R protein directs the myristylation and virion envelope localization of a heterologous fusion protein. Journal of Biological Chemistry 268:7585–7593
     [Google Scholar]
  21. Rhee S. S., Hui H., Hunter E. 1990; Preassembled capsids of type D retroviruses contain a signal sufficient for targeting specifically to the plasma membrane. Journal of Virology 64:3844–3852
     [Google Scholar]
  22. Russell R. L. Q., Rohrmann G. F. 1990; A baculovirus polyhedron envelope protein: immunogold localization in infected cells and mature polyhedra. Virology 174:177–184
     [Google Scholar]
  23. Sahli R., Freund R., Dubenski T., Garcea R., Bronson R., Benjamin T. 1993; Defect in entry and altered pathogenicity of a polyoma virus mutant blocked in VP2 myristylation. Virology 192:142–153
     [Google Scholar]
  24. Sodeik B., Doms R. W., Ericsson M., Hiller G., Machamer C. E., Vanthof W., Vanmeer G., Moss B., Griffiths G. 1993; Assembly of vaccinia virus - role of the intermediate compartment between the endoplasmic reticulum and the Golgi stacks. Journal of Cell Biology 121:521–541
     [Google Scholar]
  25. Vanslyke J. K., Hruby D. E. 1994; Immunolocalization of vaccinia virus structural proteins during virion formation. Virology 198:624–635
     [Google Scholar]
  26. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25:107–108
     [Google Scholar]
  27. Wilson I. A., Skehel J. J., Wiley D. C. 1981; Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature; London: 289366–375
     [Google Scholar]
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Characterization of the vaccinia virus L1R myristylprotein as a component of the intracellular virion envelope
J. Gen. Virol. 75, 1479 (1994); https://doi.org/10.1099/0022-1317-75-6-1479
/content/journal/jgv/10.1099/0022-1317-75-6-1479
/content/journal/jgv/10.1099/0022-1317-75-6-1479
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