RT Journal Article SR Electronic(1) A1 Leruez, M. A1 Pallier, C. A1 Vassias, I. A1 Elouet, J. F. A1 Romeo, P. A1 Morinet, F.YR 1994 T1 Differential transcription, without replication, of non-structural and structural genes of human parvovirus B19 in the UT7/EPO cell line as demonstrated by in situ hybridization JF Journal of General Virology, VO 75 IS 6 SP 1475 OP 1478 DO https://doi.org/10.1099/0022-1317-75-6-1475 PB Microbiology Society, SN 1465-2099, AB Erythroid progenitor cells are the main target for B19 parvovirus infection. The UT7 cell line demonstrates a marked erythroid differentiation on induction by erythropoietin (EPO) (UT7/EPO cells) and therefore appears to be a potential target for B19 parvovirus. We aimed to evaluate the presence and localization of B19 nucleic acids in UT7/EPO cells by in situ hybridization. Three digoxigenin-labelled probes were used: two recognized specifically the non-structural region of the B19 genome and one probe was structural region- specific. In our experiment UT7/EPO cells were not permissive to B19 infection. Transcription led to nonstructural and structural gene transcripts without DNA replication or capsid protein synthesis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-6-1475