1887

Abstract

A quantal assay, based on syncytium formation in the human T cell leukaemia-derived C8166 cell line, was used to determine the kinetics of human immunodeficiency virus type 1 (HIV-1) strain IIIB neutralization. Three rat monoclonal antibodies (MAbs) were used, under physiological conditions of temperature and antibody concentration. MAb ICR39·3b (IgG2b) neutralized virus with no lag period while the other two MAbs, ICR39·13g (IgG2b) and ICR41· 1i (IgG2a), neutralized with lag periods of 5 min and 15 min respectively. It was calculated that the latter two MAbs mediated neutralization by about two and three molecules of IgG per virion respectively. The highest neutralization rate constant (for MAb ICR 41· li) was over 300-fold less than that of MAbs specific for the haemagglutinin of the enveloped influenza virus type A and for poliovirus type 1.

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1994-06-01
2021-10-21
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References

  1. Cordell J. A., Moore J. P., Dean C. J., Klasse P. J., Weiss R. A., Mckeating J. A. 1991; Rat monoclonal antibodies to nonoverlapping epitopes of HIV-1 gpl20 block CD4 binding in vitro . Virology 185:72–79
    [Google Scholar]
  2. Dimmock N. J. 1993; Neutralization of animal viruses. Current Topics in Microbiology and Immunology 183:1–149
    [Google Scholar]
  3. Dulbecco R., Vogt M., Strickland A. G. R. 1956; A study of the basic aspects of neutralization of two animal viruses, western equine encephalitis virus and poliomyelitis virus. Virology 2:162–205
    [Google Scholar]
  4. Granoff A. 1965; The interaction of Newcastle disease virus and neutralizing antibody. Virology 25:38–47
    [Google Scholar]
  5. Icenogle J. C., Shiwen H., Duke G., Gilbert S., Rueckert R., Anderegg J. 1983; Neutralization of poliovirus by a monoclonal antibody: kinetics and stoichiometry. Virology 127:412–125
    [Google Scholar]
  6. Keller R. 1966; The stability of neutralization of poliovirus by native antibody and enzymatically derived fragments. Journal of Immunology 96:96–106
    [Google Scholar]
  7. Lafferty K. J. 1963; The interaction between virus and antibody. I. Kinetic studies. Virology 21:61–75
    [Google Scholar]
  8. Mckeating J. A., Cordell J. A., Dean C. J., Balfe P. 1992; Synergistic interaction between ligands binding to the CD4 binding site and V3 domain of human immunodeficiency virus type 1 gpl20. Virology 191:732–742
    [Google Scholar]
  9. Mandel B. 1960; Neutralization of virus infectivity: characterization of the virus-antibody complex, including association, dissociation, and host-cell interaction. Annals of the New York Academy of Sciences 83:515–527
    [Google Scholar]
  10. Mandel B. 1979; Interaction of viruses with neutralizing antibodies. In Comprehensive Virology 15 pp 37–121 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  11. Nara P. L. 1989; HIV neutralization: evidence for rapid, binding/ post-binding neutralization from infected humans, chimpanzees and gp 120-vaccinated animals. In Vaccines 89: Modern Approaches to New Vaccines Including the Prevention of AIDS pp 137–144 Lerner R. A., Ginsberg H., Chanock R. M., Brown F. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Nara P. L. 1990; Quantitative infectivity syncytium-forming microassay. In Techniques in HIV Research pp 77–86 Aldovini A., Walker B. D. Edited by New York: Stockton Press;
    [Google Scholar]
  13. Outlaw M. C., Dimmock N. J. 1991; Insights into neutralization of animal viruses gained from the study of influenza viruses. Epidemiology and Infection 106:205–220
    [Google Scholar]
  14. Philipson L. 1966; Interaction between poliovirus and immunoglobulins. II. Basic aspects of virus-antibody interaction. Virology 28:35–16
    [Google Scholar]
  15. Rubin H., Franklin R. M. 1957; On the mechanisms of Newcastle disease virus neutralization by immune serum. Virology 3:84–95
    [Google Scholar]
  16. Suñé C., Jiménez G., Correa I., Bullido M. J., Gebauer F., Smerdou C., Enjuanes L. 1990; Mechanisms of transmissible gastroenteritis coronavirus neutralization. Virology 111:559–569
    [Google Scholar]
  17. Taylor H. P., Armstrong S. J., Dimmock N. J. 1987; Quantitative relationships between an influenza virus and neutralizing antibody. Virology 159:288–298
    [Google Scholar]
  18. Thormar H. 1963; Neutralization of visna virus by antisera from sheep. Journal of Immunology 90:185–192
    [Google Scholar]
  19. Wohlfart C. 1988; Neutralization of adenoviruses: kinetics, stoichiometry and mechanisms. Journal of Virology 62:2321–2328
    [Google Scholar]
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