1887

Abstract

Budgerigar fledgling disease virus (BFDV) represents the first non-mammalian member of the polyomavirus genus and possesses uncommon structural and biological properties. Recombinant baculoviruses were constructed to express BFDV small t antigen, large T antigens, as well as a large T deletion mutant T and - galactosidase-T fusion proteins to high levels in infected insect cells. A recombinant virus containing a genomic copy of the BFDV early region was used for small t antigen expression, and corresponding intron- deleted cDNAs for production of large T antigen derivatives. Recombinant T as well as authentic T antigen proteins from infected chicken embryo fibroblasts were purified using both immunoaffinity and DNA affinity column chromatography. We present evidence that the large T antigen interacts specifically with DNA sequences present in the non-coding region of BFDV; by indirect DNA immunoprecipitation mapping and DNase I footprinting, four regions including 12 DNA-binding sites have been determined that cover most of the BFDV non-coding region. The T antigen binding pattern observed suggests a protein-DNA interaction system considerably different from those of simian virus 40 and other polyomaviruses.

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1994-06-01
2024-04-16
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Smith J. A., Seidman J. G., Struhl K. 1987 Current Protocols in Molecular Biology New York: Greene Publishing Associates and Wiley-Interscience;
    [Google Scholar]
  2. Barponetti J., Friedman P. N., Kern S. E., Vogelstein B., Prives C. 1991; Wild-type but not mutant p53 immunopurified proteins bind to sequences adjacent to the SV40 origin of replication. Cell 65:1083–1091
    [Google Scholar]
  3. Bergqvist A., Nilsson M., Bondeson K., Magnuss G. 1990; Loss of DNA-binding and new transcriptional trans-activation function in polyomavirus large T antigen with mutation of zinc finger motif. Nucleic Acids Research 18:2715–2720
    [Google Scholar]
  4. Cowie A., Kamen R. 1984; Multiple binding sites for polyomavirus large T antigen within regulatory sequences of polyoma DNA. Journal of Virology 52:750–760
    [Google Scholar]
  5. Davis R. B., Bozeman L. H., Gaudry D., Fletcher O. J., Lukert P. D., Dykstra M. J. 1981; A viral disease of fledgling budgerigars. Avian Diseases 25:179–183
    [Google Scholar]
  6. DeLucia A. L., Lewton B. A., Than R., Tegtmeyer P. 1983; Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication. Journal of Virology 46:143–150
    [Google Scholar]
  7. De Villiers J., Schaffner W., Tyndall C., Lupton S., Kamen R. 1984; Polyomavirus DNA replication requires enhancer. Nature; London: 312242–246
    [Google Scholar]
  8. Doerfler W. 1986; Expression of the Autographa californicanuclear polyhedrosis virus genome in insect cells: homologous viral and heterologous vertebrate genes - the baculovirus system. Current Topics in Microbiology and Immunology 131:51–67
    [Google Scholar]
  9. Galas D. J., Schmitz A. 1978; DNAase footprinting: a simple method for the detection of protein-DNA binding specficity. Nucleic Acids Research 5:3157–3170
    [Google Scholar]
  10. Gaudry P., Tyndall C., Kamen R., Cuzin F. 1981; The high affinity binding site on polyomavirus DNA for the viral large T antigen. Nucleic Acids Research 21:5697–5710
    [Google Scholar]
  11. Grossman S. R., Mora R., Laimins L. A. 1989; Intracellular localization and DNA-binding properties of human papillomavirus type 18 E6 protein expressed with a baculovirus vector. Journal of Virology 63:366–374
    [Google Scholar]
  12. Höss A., Moarefi I.F., Fanning E., Arthur A. K. 1990a; The finger domain of simian virus 40 large T antigen controls DNA- binding specificity. Journal of Virology 64:6291–6296
    [Google Scholar]
  13. Höss A., Moarefi I. F., Scheidtmann K., Cisek L. J., Corden J. L., Dornreiter I., Arthur A. K., Fanning E. 1990b; Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector. Journal of Virology 64:4799–4807
    [Google Scholar]
  14. Jeang K.-T., Holmgren-Konig M., Khoury G. 1987; A baculovirus vector can express intron-containing genes. Journal of Virology 61:1761–1764
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  16. Lehn H., Müller H. 1986; Cloning and characterization of budgerigar fledgling disease virus, an avian polyomavirus. Virology 151:362–370
    [Google Scholar]
  17. Leober G., Parsons R., Tegtmeyer P. 1989; The zinc finger region of simian virus 40 large T antigen. Journal of Virology 63:94–100
    [Google Scholar]
  18. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Bio/Technology 6:47–55
    [Google Scholar]
  19. Mckay R. D. G. 1981; Binding of simian virus 40 T antigen related protein to DNA. Journal of Molecular Biology 145:471–488
    [Google Scholar]
  20. Miyamoto C., Smith G. E., Farrell-Towt J., Chizzonite R., Summers M. D., Ju G. 1985; Production of human c-myc protein in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 5:2860–2865
    [Google Scholar]
  21. Müller H., Nitschke R. 1986; A polyoma-like virus associated with an acute disease of fledgling budgerigars (Melopsittacus undulatus) . Medical Microbiology and Immunology 175:1–13
    [Google Scholar]
  22. Ollo R., Maniatis T. 1987; Drosophila kriippel gene product produced in a baculovirus expression system is a nuclear phospho- protein that binds to DNA. Proceedings of the National Academy of Sciences U.S.A.: 845700–5704
    [Google Scholar]
  23. O’Reilly D. R., Miller L. K. 1988; Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells. Journal of Virology 62:3109–3119
    [Google Scholar]
  24. Pavletich N. P., Pabo C. O. 1991; Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2T Å. Science 252:809–817
    [Google Scholar]
  25. Pipas J. M. 1992; Common and unique features of T antigens encoded by the polyomavirus group. Journal of Virology 66:3979–3985
    [Google Scholar]
  26. Pomerantz B. J., Müller C. R., Hassel J. A. 1983; Polyoma virus large T antigen binds independently to multiple, unique regions on the viral genome. Journal of Virology 47:600–610
    [Google Scholar]
  27. Prives C. 1990; The replication functions of SV40 T antigen are regulated by phosphorylation. Cell 61:735–739
    [Google Scholar]
  28. Prives C., Murakami Y., Kern F. G., Folk W., Basilico C., Hurwitz J. 1987; DNA sequence requirements for replication of polyomavirus DNA in vivoand in vitro . Molecular and Cellular Biology 7:3694–3704
    [Google Scholar]
  29. Rott O., Kröger M., Müller H., Hobom G. 1988; The genome of budgerigar fledgling disease virus, an avian polyomavirus. Virology 165:74–86
    [Google Scholar]
  30. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Smith G. E., Fraser M. J., Summers M. D. 1983; Molecular engineering of the Autographa californicanuclear polyhedrosis virus genome: deletion mutations within the polyhedrin gene. Journal of Virology 46:584–593
    [Google Scholar]
  32. Stoll R., Luo D., Kouwenhoven B., Hobom G., Müller H. 1993; Molecular and biological characteristics of avian poly-omaviruses: isolates from different species of birds indicate that avian polyomaviruses form a distinct subgenus within the poly-omavirus genus. Journal of General Virology 74:229–237
    [Google Scholar]
  33. Summers M. D., Smith G. E. 1987 A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures Texas Agricultural Experiment Station Bulletin 1555
    [Google Scholar]
  34. Tegtmeyer P., Lewton B. A., DeLucia A. L., Wilson V. G., Ryder K. 1983; Topography of SV40 A protein-DNA complexes: arrangement of protein bound to the origin of replication. Journal of Virology 46:151–161
    [Google Scholar]
  35. Tenen D. G., Taylor T. S., Haines L. L., Bradley M. K., Martin R. G., Livingston D. M. 1983; Binding of simian virus 40 large T antigen from virus infected monkey cells to wild-type and mutant viral replication origins. Journal of Molecular Biology 168:791–808
    [Google Scholar]
  36. Weinberg R. A. 1991; Tumor suppressor genes. Science 254:1138–1146
    [Google Scholar]
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