1887

Abstract

The genome of pea enation mosaic virus (PEMV) is composed of two taxonomically unrelated RNAs, interacting to create what has traditionally been considered a bipartite virus. The cohesiveness of this interaction was assessed by examining the autonomy of each RNA in viral replication, coat protein expression and systemic invasion. Using a pea protoplast system, transcripts of RNA1 were found to be capable of initiating RNA 2-independent replication, including the formation of the distinctive nuclear membrane-based replication complex associated with wild-type PEMV infection. Western blotting and electron microscopic analysis demonstrated that the synthesis of the RNA1- encoded coat protein, as well as virion assembly, was also independent of RNA2-directed functions. Mechanical inoculations with transcripts of RNA 1 failed to establish a systemic RNA1 infection, whereas inoculations with RNA 2 were able to establish a largely asymptomatic systemic infection. Combined inoculum containing RNA1 and RNA2 transcripts were able to recreate wild-type PEMV symptomatology, demonstrating the dependence of RNA 1 on RNA 2 for mechanical passage. With the notable exception of the adaptation of PEMV to establish a true systemic invasion, these data further strengthen the analogy between PEMV and the helper-dependent complexes associated with members of the luteovirus group.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-5-997
1994-05-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/5/JV0750050997.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-5-997&mimeType=html&fmt=ahah

References

  1. Bloch K. D., Bartos B. 1993; Partial digestion of DNA with restriction endonucleases. In Current Protocols in Molecular Biology pp 3.1.3–3.1.4 Ausubel F. M., Brent R., Kingston R., Moore D. D., Seidman J. G., Smith J. A., Struhl K. Edited by New York: John Wiley;
    [Google Scholar]
  2. Demler S. A., Dezoeten G. A. 1989; Characterization of a satellite RNA associated with pea enation mosaic virus. Journal of General Virology 70:1075–1084
    [Google Scholar]
  3. Demler S. A., De Zoeten G. A. 1991; The nucleotide sequence and luteovirus-like nature of RNA 1 of an aphid non-transmissible strain of pea enation mosaic virus. Journal of General Virology 72:1819–1834
    [Google Scholar]
  4. Demler S. A., Rucker D. G., De Zoeten G. A. 1993; The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA2. Journal of General Virology 74:1–14
    [Google Scholar]
  5. De Zoeten G. A., Gaard G. 1983; Mechanisms underlying systemic invasion of pea plants by pea enation mosaic virus. Intervirology 19:85–94
    [Google Scholar]
  6. De Zoeten G. A., Gaard G., Diez F. B. 1972; Nuclear vesiculation associated with pea enation mosaic virus-infected plant tissue. Virology 48:638–647
    [Google Scholar]
  7. De Zoeten G. A., Powell C. A., Gaard G., German T. L. 1976; In situ localization of pea enation mosaic virus double-stranded ribonucleic acid. Virology 70:459–469
    [Google Scholar]
  8. Esau K., Hoefert L. L. 1972; Development of infection with beet western yellows virus in the sugarbeet. Virology 48:724–738
    [Google Scholar]
  9. Falk B. W., Duffus J. E. 1981; Epidemiology of helper-dependent persistent aphid transmitted virus complexes. In Plant Diseases and Vectors pp 161–179 Maramorosch K., Harris K. F. Edited by New York: Academic Press;
    [Google Scholar]
  10. Falk B. W., Duffus J. E., Morris T. J. 1979; Transmission, host range and serological properties of the viruses causing lettuce speckles disease. Phytopathology 69:612–617
    [Google Scholar]
  11. Francki R. I. B., Milne R. G., Hatta T. 1985; Tobacco necrosis virus. In Atlas of Plant Viruses 1: pp 171–180 Boca Raton: CRC Press;
    [Google Scholar]
  12. Gibbs A. J., Harrison B. D., Woods R. D. 1966; Purification of pea enation mosaic virus. Virology 29:348–351
    [Google Scholar]
  13. Gill C. C., Chong J. 1976; Differences in cellular ultrastructural alterations between variants of barley yellow dwarf virus. Virology 75:33–47
    [Google Scholar]
  14. Hull R. 1977; Properties of an aphid-borne virus: pea enation mosaic virus. In Aphids as Virus Vectors pp 137–162 Harris K. F., Maramorosch K. Edited by New York: Academic Press;
    [Google Scholar]
  15. Hull R., Lane L. C. 1973; The unusual nature of the components of a strain of pea enation mosaic virus. Virology 55:1–13
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  17. Miller W. A., Waterhouse P. M., Kortt A. A., Gerlach W. L. 1988; Sequence and identification of the barley yellow dwarf virus coat protein gene. Virology 165:306–309
    [Google Scholar]
  18. Murant A. F. 1993; Complexes of transmission-dependent and helper viruses. In Diagnosis of Plant Virus Diseases pp 334–357 Matthews R. E. F. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  19. Powell C. A., De Zoeten G. A. 1977; Replication of pea enation mosaic virus RNA in isolated pea nuclei. Proceedings of the National Academy of Sciences U.S.A: 742919–2922
    [Google Scholar]
  20. Powell C. A., De Zoeten G. A., Gaard G. 1977; The localization of pea enation mosaic virus-induced RNA-dependent RNA polymerase in infected peas. Virology 78:135–143
    [Google Scholar]
  21. Reinke K. J., De Zoeten G. A. 1991; In situ localization of viral gene products. Phytopathology 81:1306–1314
    [Google Scholar]
  22. Reutenauer A., Ziegler-Graff V., Lot H., Scheidecker D., Guilley H., Richards K., Jonard G. 1993; Identification of beet western yellows luteovirus genes implicated in viral replication and particle morphogenesis. Virology 195:692–699
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Shepardson S., Esau K., Mccrum R. 1980; Ultrastructure of potato leaf phloem infected with potato leafroll virus. Virology 105:379–392
    [Google Scholar]
  25. Tacke E., Prüfer D., Schmitz J., Rohde W. 1991; The potato leafroll luteovirus 17K protein is a single-stranded nucleic acidbinding protein. Journal of General Virology 72:2035–2038
    [Google Scholar]
  26. Van Der Wilk F., Huisman M. J., Cornelissen B. J. C., Huttinga H., Goldbach R. 1989; Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Letters 245:51–56
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-5-997
Loading
/content/journal/jgv/10.1099/0022-1317-75-5-997
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error