1887

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Volume 75, Issue 5

Corticosteroid Immunosuppression and Monoclonal Antibody-mediated CD5 T Lymphocyte Depletion in Normal and Equine Infectious Anaemia Virus-carrier Horses Free

Abstract

The immune control of chronic equine infectious anaemia (EIA) lentiviral infection was investigated by specifically depleting CD5 T lymphocytes with monoclonal antibody (MAb) or by immunosuppression with corticosteroids. MAb was given at 25 to 50 mg/day intravenously for 11 days. Murine IgG1 anti-equine CD2 MAb ( = 2 horses) or IgG1 ( = 2) and IgG2a control MAb ( = 2 normal; 2 EIA-infected) did not deplete CD2 T lymphocytes in horses. Horses given murine IgG2a anti-CD5 MAb HB19A ( = 4 normal; 5 EIA-infected) had depletion of peripheral blood CD5 T lymphocytes during treatment. These horses, however, maintained a residual population of CD2 T lymphocytes [15 ( ± 3)% of pretreatment numbers] that did not express CD5 but expressed either CD4 or CD8. These antigenically modulated CD5 T lymphocytes responded normally to intradermal inoculation with phyto-haemagglutinin and to allogeneic leukocyte stimulation in one-way mixed lymphocyte reactions. EIA virus-infected horses ( = 5) did not develop recrudescent viraemia or disease following CD5 T lymphocyte depletion. Immunosuppression of EIA virus-infected horses with corticosteroids (1 mg/kg body weight/day, intravenously for 9 days) resulted in detectable recrudescent EIA viraemia in 6/11 horses (55%) and recrudescent disease in 9/11 horses (82%). Normal horses ( = 3) treated with corticosteroids developed no clinical disease. These results demonstrate that the use of murine IgG2a MAbs to appropriate equine lymphocyte antigens will facilitate investigation of the role of T lymphocyte subpopulations in the control of EIA or other important equine diseases.

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© The Journal of General Virology 1994
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1994-05-01
2024-04-19
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References

  1. Banks K. L., Henson J. B., Mcguire T. C. 1972; Immunologically mediated glomerulitis of horses. I. Pathogenesis in persistent infection by equine infectious anemia virus. Laboratory Investigations 6:701–707
     [Google Scholar]
  2. Chatenoud L., Baudrihaye M. F., Kreis H., Goldstein G., Schindler J., Bach J. F. 1982; Human in vivo antigenic modulation induced by the anti-T cell OKT3 monoclonal antibody. European Journal of Immunology 12:979–982
     [Google Scholar]
  3. Cheevers W. P., Mcguire T. C. 1985; Equine infectious anemia virus: immunopathogenesis and persistence. Reviews of Infectious Diseases 7:83–88
     [Google Scholar]
  4. Clabough-Sellon D., Perry S. T., Coggins L., Fuller F. J. 1992; Wild-type equine infectious anemia virus replicates in vivo in tissue macrophages, not peripheral blood monocytes. Journal of Virology 66:5906–5913
     [Google Scholar]
  5. Clements J. E., Gdovin S. C., Montelaro R. C., Narayan O. 1988; Antigenic variation in lentiviral diseases. Annual Review of Immunology 6:139–159
     [Google Scholar]
  6. Cobbold S. P., Jayasuriya A., Nash A., Prospero T. D., Waldmann H. 1984; Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature; London: 312548–551
     [Google Scholar]
  7. Coggins L., Norcross N. L., Nussbaum S. R. 1972; Diagnosis of equine infectious anemia by immunodiffusion test. American Journal of Veterinary Research 33:11–18
     [Google Scholar]
  8. Collman R., Godfrey B., Cutilli J., Rhodes A., Hassan N. F., Sweet R., Douglas S. D., Friedman H., Nathanson N., Gonzalez-Scarano F. 1990; Macrophage-tropic strains of human immunodeficiency virus type 1 utilize the CD4 receptor. Journal of Virology 64:4468–4476
     [Google Scholar]
  9. Crump A. L., Davis W., Antczak D. F. 1988; A monoclonal antibody identifying a T-cell marker in the horse. Animal Genetics 19:349–357
     [Google Scholar]
  10. Davis W. C., Mcguire T. C., Perryman L. E. 1983; Biomedical and biological application of monoclonal antibody technology in developing countries. Periodicum Biologorum 85:259–281
     [Google Scholar]
  11. Davis W. C., Marusic S., Lewin H. A., Splitter G., Perryman L. E., Mcguire T. C., Gorham J. R. 1987; The development and analysis of species-specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Veterinary Immunology and Immunopathology 15:337–376
     [Google Scholar]
  12. Hodgin C. E., Mcguire T. C., Perryman L. E., Grant B. D. 1978; Evaluation of delayed hypersensitivity response in normal and immunodeficient foals. American Journal of Veterinary Research 39:1161–1167
     [Google Scholar]
  13. Hoffman R. A., Kung P. C., Hansen W. P., Goldstein G. 1980; Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proceedings of the National Academy of Sciences, U.S.A. 774914–4917
     [Google Scholar]
  14. Howard C. J., Sopp P., Parsons K. R., Finch J. 1989; In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. European Journal of Immunology 19:757–764
     [Google Scholar]
  15. Issel C. J., Horohov D. W., Lea D. F., Adams W. V., Hagius S. D., Mcmanus J. M., Allison A. C., Montelaro R. C. 1992; Efficacy of inactivated whole-virus and subunit vaccines in preventing infection and disease caused by equine infectious anemia virus. Journal of Virology 66:3398–3408
     [Google Scholar]
  16. Jonker M., Goldstein G., Balner H. 1983; Effects of in vivo administration of monoclonal antibodies for human T cell subpopulations on the immune system in a rhesus monkey model. Transplantation 35:521–526
     [Google Scholar]
  17. Knapp W., Dorken B., Gilks W. R., Rieber E. P., Schmidt R. E., Stein H., Vondemborne A.E.G.KR.Editors 1989 Leukocyte Typing IV, White Cell Differentiation Antigens Oxford: Oxford University Press;
     [Google Scholar]
  18. Kono Y., Fukunaga Y., Kobayashi K. 1973; Resistance of horses infected chronically with equine infectious anemia virus against reinfection. National Institute of Animal Health Quarterly 13:173–181
     [Google Scholar]
  19. Kono Y., Hirasawa K., Fukunaga Y., Taniguchi T. 1975; Recrudescence of equine infectious anemia by treatment with immunosuppressive drugs. National Institute of Animal Health Quarterly 16:667–674
     [Google Scholar]
  20. Kydd J. H., Antczak D. F. 1991; First International Workshop on Equine Leukocyte Antigens 12th-13th July, 1991: preliminary report. Equine Veterinary Journal supplement 12:4–6
     [Google Scholar]
  21. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. 1981; Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. Journal of Experimental Medicine 153:310–323
     [Google Scholar]
  22. Lunn D. P. 1993; A comparative review of human and equine leucocyte differentiation antigens. British Veterinary Journal 149:31–19
     [Google Scholar]
  23. Lunn D. P., Holmes M. A., Duffus W. P. 1991; Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunology 74:251–257
     [Google Scholar]
  24. Mcguire T. C., Crawford T. B., Henson J. B. 1971; Immuno-fluorescent localization of equine infectious anemia virus in tissue. American Journal of Pathology 62:283–292
     [Google Scholar]
  25. Michaelides M., Hogarth P. M., Mckenzie I. F. C. 1981; The immunosuppressive effect of monoclonal anti-Lyt-1.1 antibodies in vivo. European Journal of Immunology 11:1005–1012
     [Google Scholar]
  26. Montelaro R. C., Robey W. G., West M. D., Issel C. J., Fischinger P. J. 1988; Characterization of the serological crossreactivity between glycoproteins of the human immunodeficiency virus and equine infectious anaemia virus. Journal of General Virology 69:1711–1717
     [Google Scholar]
  27. O’Rourke K., Perryman L. E., Mcguire T. C. 1988; Antiviral, anti-glycoprotein and neutralizing antibodies in foals with equine infectious anaemia virus. Journal of General Virology 69:667–674
     [Google Scholar]
  28. Palmer G. H., Mcguire T. C. 1984; Immune serum against Anaplasma marginale initial bodies neutralizes infectivity for cattle. Journal of Immunology 133:1010–1015
     [Google Scholar]
  29. Payne S. L., Fang F., Liu C., Dhruva B. R., Rwambo P., Issel C. J., Montelaro R. C. 1987; Antigenic variation and lentivirus persistence: variations in envelope gene sequences during EIAV infection resemble changes reported for sequential isolates of HIV. Virology 161:321–331
     [Google Scholar]
  30. Perryman L. E., Mcguire T. C. 1978; Mixed lymphocyte responses in combined immunodeficiency of horses. Transplantation 25:50–52
     [Google Scholar]
  31. Perryman L. E., O’Rourke K., Mcguire T. C. 1988; Immune responses are required to terminate viremia in equine infectious anemia lentivirus infection. Journal of Virology 62:3073–3076
     [Google Scholar]
  32. Poppema S., Bhan A. K., Reinherz E. L., Mccluskey R. T., Schlossman S. F. 1981; Distribution of T cell subsets in human lymph nodes. Journal of Experimental Medicine 153:30–41
     [Google Scholar]
  33. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
     [Google Scholar]
  34. Rushlow K., Olsen K., Stiegler G., Payne S. L., Montelaro R. C., Issel C. 1986; Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus. Virology 155:309–321
     [Google Scholar]
  35. Stephens R. M., Casey J. W., Rice N. R. 1986; Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science 231:589–594
     [Google Scholar]
  36. Suarez C. E., Palmer G. H., Jasmer D. P., Hines S. A., Perryman L. E., Mcelwain T. F. 1991; Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes. Molecular and Biochemical Parasitology 46:45–52
     [Google Scholar]
  37. Titus R. G., Milon G., Marchal G., Vassalli P., Cerottini J. C., Louis J. A. 1987; Involvement of specific Lyt-2+ T-cells in the immunological control of experimentally induced murine cutaneous leishmaniasis. European Journal of Immunology 17:1429–1433
     [Google Scholar]
  38. Van Ewijk W., Van Soest P. L., Van Den Engh J. 1981; Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2 and T-200. Journal of Immunology 127:2594–2604
     [Google Scholar]
  39. Waldmann H. 1989; Manipulation of T-cell responses with monoclonal antibodies. Annual Review of Immunology 7:407–444
     [Google Scholar]
  40. Weiss W. R., Wyatt C. R., Davis W. C., Mcguire T. C., Perryman L. E. 1988; T lymphocyte development in horses. I. Characterization of monoclonal antibodies identifying three stages of lymphocyte differentiation. Veterinary Immunology and Immunopathology 18:3–18
     [Google Scholar]
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Corticosteroid Immunosuppression and Monoclonal Antibody-mediated CD5+ T Lymphocyte Depletion in Normal and Equine Infectious Anaemia Virus-carrier Horses
J. Gen. Virol. 75, 959 (1994); https://doi.org/10.1099/0022-1317-75-5-959
/content/journal/jgv/10.1099/0022-1317-75-5-959
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