1887

Abstract

Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BYDY) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23 % of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35 %). A higher proportion of CD2 T cells (23 %) were infected, compared with B cells (11 %) or WC1 T cells (11 %). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.

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1994-05-01
2022-01-22
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