RT Journal Article SR Electronic(1) A1 Ponimaskin, Evgeni A1 Veit, Michael A1 Schmidt, Michael F. G.YR 1994 T1 Expression of the Sendai Virus Fusion Protein in Insect Cells and Characterization of its Post-translational Modifications JF Journal of General Virology, VO 75 IS 5 SP 1163 OP 1167 DO https://doi.org/10.1099/0022-1317-75-5-1163 PB Microbiology Society, SN 1465-2099, AB The fusion (F) protein of Sendai vims was expressed with a baculovirus system in insect cells. The F protein is synthesized in its uncleaved form F0, which appears in SDS-containing gels as two bands with M r values of 66K and 64K. Digestion of the F protein with endo-glycosidase H revealed that the 66K species contains high mannose-type carbohydrates, whereas partially processed oligosaccharides are attached to the 64K species. Pulse-chase experiments showed that the F protein is initially synthesized as its 66K form. After 1 h of chase this precursor glycoprotein is partially converted to the 64K species and exposed at the cell surface. Other modifications of the F protein in insect cells are the formation of intramolecular disulphide linkages and oligomerization. However, processing and plasma membrane transport of the F protein in insect cells are incomplete and retarded compared to the F protein synthesized in Sendai virus-infected mammalian cells., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-5-1163