1887

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Volume 75, Issue 5

Replication of Cucumber Mosaic Virus Satellite RNA from Negative-sense Transcripts Produced Either or in Transgenic Plants Free

Abstract

Both positive [( + )] and negative [(−)] sense versions of two satellite RNA (satRNA) genes from cucumber mosaic virus (CMV), the necrogenic I17N and the non- necrogenic R, have been introduced into the genome of tobacco plants. On infection with satRNA-free CMV, satRNA was amplified in plants expressing each of the four genes. All four genes confer protection against CMV infection. However, co-inoculation of plants with viral RNA and CMV satRNA transcripts synthesized showed that (−) sense transcripts were less active than the corresponding (+) sense transcripts. This is the first report that (−) sense CMV satRNA transcripts can serve as a template for satRNA replication.

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© The Journal of General Virology 1994
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1994-05-01
2024-04-25
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Replication of Cucumber Mosaic Virus Satellite RNA from Negative-sense Transcripts Produced Either in Vitro or in Transgenic Plants
J. Gen. Virol. 75, 1009 (1994); https://doi.org/10.1099/0022-1317-75-5-1009
/content/journal/jgv/10.1099/0022-1317-75-5-1009
/content/journal/jgv/10.1099/0022-1317-75-5-1009
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