1887

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Volume 75, Issue 5

Replication of Cucumber Mosaic Virus Satellite RNA from Negative-sense Transcripts Produced Either or in Transgenic Plants Free

Abstract

Both positive [( + )] and negative [(−)] sense versions of two satellite RNA (satRNA) genes from cucumber mosaic virus (CMV), the necrogenic I17N and the non- necrogenic R, have been introduced into the genome of tobacco plants. On infection with satRNA-free CMV, satRNA was amplified in plants expressing each of the four genes. All four genes confer protection against CMV infection. However, co-inoculation of plants with viral RNA and CMV satRNA transcripts synthesized showed that (−) sense transcripts were less active than the corresponding (+) sense transcripts. This is the first report that (−) sense CMV satRNA transcripts can serve as a template for satRNA replication.

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© The Journal of General Virology 1994
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1994-05-01
2022-01-27
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References

  1. Baulcombe D. C., Saunders G. R., Bevan M. W., Mayo M. A., Harrison B. D. 1986; Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants. Nature; London: 321446–449
     [Google Scholar]
  2. Chirgwin J. M., Przybyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases. Biochemistry 18:5294–5299
     [Google Scholar]
  3. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
     [Google Scholar]
  4. Collmer C. W., Kaper J. M. 1985; Double-stranded RNAs of cucumber mosaic virus and its satellite contain an unpaired terminal guanosine: implications for replication. Virology 145:249–259
     [Google Scholar]
  5. Collmer C. W., Kaper J. M. 1986; Infectious RNA transcripts from cloned cDNAs of cucumber mosaic viral satellites. Biochemical and Biophysical Research Communications 135:290–296
     [Google Scholar]
  6. Diaz-Ruiz J. R., Kaper J. M. 1977; Cucumber mosaic virus- associated RNA-5. III. Little nucleotide sequence homology between CARNA-5 and helper RNA. Virology 80:204–213
     [Google Scholar]
  7. Douine L., Quiot J. B., Marchoux G., Archange P. 1979; Recensement des espèces végétales sensibles au virus de la mosaïque du concombre. Etude bibliographique. Annales de Phytopathologie 11:439–175
     [Google Scholar]
  8. Gonsalves D., Provvidenti R., Edwards M. C. 1982; Tomato white leaf: the relation of an apparent satellite RNA and cucumber mosaic virus. Phytopathology 72:1533–1538
     [Google Scholar]
  9. Gould A. R., Palukaitis P., Symons R. H., Mossop D. W. 1978; Characterization of a satellite RNA associated with cucumber mosaic virus. Virology 84:443–455
     [Google Scholar]
  10. Hayes R. J., Buck K. W. 1990; Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368
     [Google Scholar]
  11. Hayes R. J., Tousch D., Jacquemond M., Pereira V. C., Buck K. W., Tepfer M. 1992; Complete replication of a satellite RNA in vitro by a purified RNA-dependent RNA polymerase. Journal of General Virology 73:1597–1600
     [Google Scholar]
  12. Jacquemond M. 1982; Phénomènes d’intérference entre les types d’ARN satellite du virus de la mosaïque du concombre. Protection des tomates vis à vis de la nécrose létale. Comptes Rendus de 1’Academie des Sciences de Paris 294:991–994
     [Google Scholar]
  13. Jacquemond M., Lauquin G. J. M. 1988; The cDNA of cucumber mosaic virus-associated satellite RNA has in vivo biological properties. Biochemical and Biophysical Research Communications 151:388–395
     [Google Scholar]
  14. Jacquemond M., Leroux J. P. 1982; L’ARN satellite du virus de la mosaïque du concombre. II. Etude de la relation virus-ARN satellite chez divers hôtes. Agronomie 2:55–62
     [Google Scholar]
  15. Jacquemond M., Lot H. 1981; L’ARN satellite du virus de la mosaïque du concombre. I. Comparaison de l’aptitude à induire la nécrose de la tomate d’ARN satellites isolés de plusieurs souches du virus. Agronomie 1:927–932
     [Google Scholar]
  16. Jacquemond M., Amselem J., Tepfer M. 1988; A gene coding for a monomeric form of cucumber mosaic virus satellite RNA confers tolerance to CMV. Molecular Plant-Microbe Interactions 1:311–316
     [Google Scholar]
  17. Kaper J. M., Tousignant M. E. 1977; Cucumber mosaic virus- associated RNA-5. I. Role of host plants and helper strains in determining amount associated with virions. Virology 80:186–195
     [Google Scholar]
  18. Kaper J. M., Waterworth H. E. 1977; Cucumber mosaic vims associated RNA-5: causal agent for tomato necrosis. Science 196:429–431
     [Google Scholar]
  19. Kaper J. M., Tousignant M. E., Thompson S. M. 1981; Cucumber mosaic virus-associated RNA-5. VIII. Identification and partial characterization of a CARNA-5 incapable of inducing tomato necrosis. Virology 114:526–533
     [Google Scholar]
  20. Kay R., Chan A., Daly M., Mcpherson J. 1987; Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236:1299–1302
     [Google Scholar]
  21. Kurath G., Palukaitis P. 1987; Biological activity of T7 transcripts of a prototype clone and a sequence variant clone of a satellite RNA of cucumber mosaic virus. Virology 159:199–208
     [Google Scholar]
  22. Lehrach H., Diamond-Wozney J. M., Boedtker H. 1977; RNA molecular weight determination by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
     [Google Scholar]
  23. Lot H., Marrou J., Quiot J. B., Esvan C. 1972; Contribution à l’étude du virus de la mosaïque du concombre. I. Méthode de purification rapide du virus. Annales de Phytopathologie 4:25–38
     [Google Scholar]
  24. Lot H., Jonard G., Richards K. E. 1977; Cucumber mosaic virus RNA-5. Partial characterization and evidence for no large sequence homologies with genomic RNAs. FEBS Letters 80:395–400
     [Google Scholar]
  25. Mcgarvey P. B., Kaper J. M., Avila-Rincon M. J., Pena L., Diaz-Ruiz J. R. 1990; Transformed tomato plants express a satellite RNA of cucumber mosaic virus and produce lethal necrosis upon infection with viral RNA. Biochemical and Biophysical Research Communications 170:548–555
     [Google Scholar]
  26. Masuta C., Kuwata S., Takanami Y. 1988; Effects of extra 5′ non-viral bases on the infectivity of transcripts from a cDNA clone of satellite RNA (strain Y) of cucumber mosaic virus. Journal of Biochemistry 104:841–846
     [Google Scholar]
  27. Masuta C., Komari T., Takanami Y. 1989; Expression of cucumber mosaic virus satellite RNA from cDNA copies in transgenic tobacco plants. Annals of the Phytopathological Society of Japan 55:49–55
     [Google Scholar]
  28. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
     [Google Scholar]
  29. Mossop D. W., Francki R. I. B. 1979; Comparative studies on two satellite RNAs of cucumber mosaic virus. Virology 95:395–104
     [Google Scholar]
  30. Palukaitis P. 1988; Pathogenicity regulation by satellite RNAs of cucumber mosaic virus: minor nucleotide sequence changes alter host responses. Molecular Plant-Microbe Interactions 1:175–181
     [Google Scholar]
  31. Robaglia C., Vilaine F., Pautot V., Raimond F., Amselem J., Jouanin L., Casse-DELBART F., Tepfer M. 1987; Expression vectors based on Agrobacterium rhizogenes Ri plasmid transformation system. Biochimie 69:231–237
     [Google Scholar]
  32. Saito Y., Komari T., Masuta C., Hayashi Y., Kumashori T., Takanami Y. 1992; Cucumber mosaic virus-tolerant transgenic tomato plants expressing a satellite RNA. Theoretical and Applied Genetics 83:679–683
     [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  34. Takanami Y. 1981; A striking change in symptoms on cucumber mosaic virus-infected tobacco plants induced by a satellite RNA. Virology 109:120–126
     [Google Scholar]
  35. Tepfer M., Casse-Delbart F. 1987; Agrobacterium rhizogenes as a vector for transforming higher plants. Microbiological Sciences 4:24–28
     [Google Scholar]
  36. Tousch D., Jacquemond M., Tepfer M. 1990; Transgenic tomato plants expressing a cucumber mosaic virus (CMV) satellite RNA gene: inoculation with CMV induces lethal necrosis. Comptes Rendus de l’ Académie des Sciences de Paris 331:377–384
     [Google Scholar]
  37. Waterworth H. E., Kaper J. M., Tousignant M. E. 1979; CARNA-5, the small cucumber mosaic virus-dependent replicating RNA, regulates disease expression. Science 204:845–847
     [Google Scholar]
  38. Wu G., Kaper J. M., Jaspars E. M. J. 1991; Replication of cucumber mosaic virus satellite RNA in vitro by an RNA-dependent RNA polymerase from virus-infected tobacco. FEBS Letters 292:213–216
     [Google Scholar]
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Replication of Cucumber Mosaic Virus Satellite RNA from Negative-sense Transcripts Produced Either in Vitro or in Transgenic Plants
J. Gen. Virol. 75, 1009 (1994); https://doi.org/10.1099/0022-1317-75-5-1009
/content/journal/jgv/10.1099/0022-1317-75-5-1009
/content/journal/jgv/10.1099/0022-1317-75-5-1009
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