1887

Abstract

Strand-specific riboprobes representative of either strand of the chicken anaemia virus (CAV) replicative form (RF) DNA indicated that only one strand of the RF was transcribed to produce a major 2·0 kb transcript and that the encapsidated DNA strand was of negative sense. Primer extension analysis located a single transcriptional start site at nucleotide position 360 of the CAV sequence. Amplification, cloning and sequencing of the 3′ end of the major transcript revealed the polyadenylation site at nucleotide position 21. Northern blot analysis using a series of genomic probes indicated that the 2·0 kb transcript was devoid of splicing and identified a non-transcribed region of the genome. This non-transcribed region was shown to possess promoter activity, enhancing the expression of the human growth hormone reporter gene in a transient gene expression assay. These observations suggest a simple strategy of genome expression involving a functional polycistronic message.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-4-905
1994-04-01
2024-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/4/JV0750040905.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-4-905&mimeType=html&fmt=ahah

References

  1. Claessens J. A. J., Schrier C. C., Mockett A. P. A., Jagt E. H. J. M., Sondermeijer P. A. J. 1991; Molecular cloning and sequence analysis of the genome of chicken anaemia agent. Journal of General Virology 72:2003–2006
    [Google Scholar]
  2. Feinberg A., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  3. Frohman M. A., Dush M. K., Martin G. R. 1988; Rapid production of full length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proceedings of the National Academy of Sciences, U.S.A 85:8998–9002
    [Google Scholar]
  4. Gelderblom H., Kling S., Lurz R., Tischer I., von Bülow V. 1989; Morphological characterization of chicken anaemia agent (CAA). Archives of Virology 109:115–120
    [Google Scholar]
  5. Mcnulty M. S. 1991; Chicken anaemia agent (CAA): a review. Avian Pathology 20:187–203
    [Google Scholar]
  6. Mcnulty M. S., Connor T. J., Mcneilly F. M., Kirkpatrick K. S., Mcferran J. B. 1988; A serological survey of domestic poultry in the United Kingdom for antibody to chicken anaemia agent. Avian Pathology 17:315–324
    [Google Scholar]
  7. Mcnulty M. S., Curran W. L., Todd D., Mackie D. P. 1990; Chicken anaemia agent: an electron microscope study. Avian Diseases 34:736–743
    [Google Scholar]
  8. Mankertz J., Buhk H.-J. 1990; Transcriptional analysis of porcine circovirus (PCV). In Abstracts, VIIIth International Congress of Virology, Berlin, August 26-31, 1990 P54-008 p. 380
    [Google Scholar]
  9. Meehan B. M., Todd D., Creelan J. L., Earle J. A. P., Hoey E. M., Mcnulty M. S. 1992; Characterisation of viral DNAs from cells infected with chicken anaemia agent: sequence analysis of the cloned replicative form and transfection capabilities of the cloned genome fragments. Archives of Virology 124:301–319
    [Google Scholar]
  10. Noteborn M. H. M., de Boer G. F., van Roozelaar D. J., Karreman C., Kranenburg O., Vos J. G., Jeurissen S. H. M., Hoeben R. C., Zantema A., Koch G., van Ormondt H., van der Eb A. J. 1991; Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. Journal of Virology 65:3131–3139
    [Google Scholar]
  11. Noteborn M. H. M., Kranenburg O., Zantema A., Koch G., de Boer G. F., van der Eb A. J. 1992; Transcription of the chicken anaemia virus (CAV) genome and the synthesis of its 52kDa protein. Gene 118:267–271
    [Google Scholar]
  12. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Selden R. F., Burke Howie K., Rowe M. E., Goodman H. M., Moore D. D. 1986; Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Molecular and Cellular Biology 6:3173–3179
    [Google Scholar]
  14. Simon J. A., Lis J. T. 1987; A germline transformation analysis reveals flexibility in the organisation of heat shock consensus elements. Nucleic Acids Research 15:2971–2988
    [Google Scholar]
  15. Sompayrac L. M., Danna K. J. 1981; Efficient infection of monkey cells with DNA of simian virus 40. Proceedings of the National Academy of Sciences, U.S.A 78:7575–7578
    [Google Scholar]
  16. Studdert M. J. 1993; Circoviridae: new viruses of pigs, parrots and chickens. Australian Veterinary Journal 70:121–122
    [Google Scholar]
  17. Todd D., Creelan J. L., Mackie D. P., Rixon F., Mcnulty M. S. 1990; Purification and biochemical characterization of chicken anaemia agent. Journal of General Virology 71:819–823
    [Google Scholar]
  18. Todd D., Creelan J. L., Mcnulty M. S. 1991; Dot blot hybridization assay for chicken anaemia agent using a cloned DNA probe. Journal of Clinical Microbiology 29:933–939
    [Google Scholar]
  19. von Bülow V., Fuchs B., Vielitz E., Landgraf H. 1983; Fruhsterblichkeitssyndrom bei kuken nach doppelinfektion mit dem virus der Marekschen krankheit (MDV) und einemanamie-erreger (CAA). Zentralblatt für Veterinärmediiin B30:742–750
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-75-4-905
Loading
/content/journal/jgv/10.1099/0022-1317-75-4-905
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error