1887

Abstract

Bovine herpesvirus 1 (BHV-1) glycoprotein gill functions both as a major virus attachment protein and haemagglutinating protein. Here we constructed recombinant baculovirus incorporating the BHV-1 gill coding sequence to characterize the expression, function and immunogenicity of the glycoprotein in insect cells. The recombinant gill had an of 72K and seemed to form homodimers. The gill was expressed on the surface of insect cells and a rosette formation assay demonstrated haemadsorbing activity of the glycoprotein. Antigenic authenticity of the recombinant gill was confirmed by a panel of monoclonal antibodies specific for the glycoprotein produced in mammalian cells. Antisera raised to recombinant gill neutralized the infectivity of BHV-1. These data suggest that recombinant gill produced in insect cells may be a useful immunogen in a BHV-1 vaccine.

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1994-04-01
2022-05-28
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References

  1. Allen G. P., Coogle L. D. 1988; Characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gp 13) with homology to herpes simplex virus glycoprotein C. Journal of Virology 62:2850–2858
    [Google Scholar]
  2. Babiuk L. A., L’Italien J., van Drunen Littel-van den Hurk S., Zamb T., Lawman M. J. P., Hughes G., Gifford G. A. 1987; Protection of cattle from bovine herpesvirus 1 (BHV-1) infection by immunization with individual viral glycoproteins. Virology 159:57–66
    [Google Scholar]
  3. Binns M. M., Ross N. L. J. 1989; Nucleotide sequence of the Marek’s disease virus (MDV) RB-1B A antigen and the identification of the MDV A antigen as the herpes simplex virus-1 glycoprotein C homologue. Virus Research 12:371–382
    [Google Scholar]
  4. Cole G. J., Loewy A., Glaser L. 1986; Neural cell-cell adhesion depends on interactions of N-CAM with heparinlike molecules. Nature; London: 320445–447
    [Google Scholar]
  5. Copeland C. S., Doms R. W., Bolzau E. M., Webster R. G., Helenius A. 1986; Assembly of influenza hemagglutinin trimers and its role in intracellular transport. Journal of Cell Biology 103:1179–1191
    [Google Scholar]
  6. Fitzpatrick D. R., Babiuk L. A., Zamb T. J. 1989; Nucleotide sequence of bovine herpesvirus type 1 glycoprotein gill, a structural model for gill as a new member of the immunoglobulin superfamily, and implications for the homologous glycoproteins of other herpesviruses. Virology 173:46–57
    [Google Scholar]
  7. Herold B. C., Wudunn D., Soltys N., Spear P. G. 1991; Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in mfectivity. Journal of Virology 65:1090–1098
    [Google Scholar]
  8. Hink W. F., Thomsen D. R., Davidson D. J., Meyer A. L., Castellino F. J. 1991; Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress 7:9–14
    [Google Scholar]
  9. Hutchinson L., Browne H., Wargent V., Davis-Poynter N., Primorac S., Goldsmith K. M., Minson A. C., Johnson D. C. 1992; A novel herpes simplex virus glycoprotein, gL. forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH. Journal of Virology 66:2240–2250
    [Google Scholar]
  10. Kinchington P. R., Remenick J., Ostrove J. M., Straus S. E., Ruyechan W. T., Hay J. 1986; Putative glycoprotein gene of varicella-zoster virus with variable copy number of a 42-base-pair repeat sequence has homology to herpes simplex virus glycoprotein C. Journal of Virology 59:660–668
    [Google Scholar]
  11. Kreis T. E., Lodish H. F. 1986; Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface. Cell 46:929–937
    [Google Scholar]
  12. Kuroda K., Geyer H., Geyer R., Doerfler W., Klenk H.-D. 1990; The oligosaccharide of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector. Virology 174:418–429
    [Google Scholar]
  13. Laterra J., Silbert D. C., Culp L. A. 1983; Cell surface heparan sulfate mediates some adhesive response to glycosaminoglycan-binding matrices, including fibronectin. Journal of Cell Biology 96:112–123
    [Google Scholar]
  14. Liang X., Babiuk L. A., van Drunen Littel-van den Hurk S., Fitzpatrick D. R., Zamb T. J. 1991; Bovine herpesvirus 1 attachment to permissive cells is mediated by its major glycoproteins gI, gIII, and gIV. Journal of Virology 65:1124–1132
    [Google Scholar]
  15. Liang X., Babiuk L. A., Zamb T. J. 1992; An in vitro study of glycoprotein gill-negative bovine herpesvirus 1 (BHV-1) mutant expressing β-galactosidase: evaluation of the role of gill in virus infectivity and its use as a vector for mucosal immunization. Virology 189:629–639
    [Google Scholar]
  16. Marshall R. L., Rodriguez L. L., Letchworth G. J. III 1986; Characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immuno-logical methods. Journal of Virology 57:745–753
    [Google Scholar]
  17. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirement for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  18. Mettenleiter T. C., Zsak L., Zukermann F., Sugg N., Kern H., Ben-Porat T. 1990; Interaction of glycoprotein gIII with a cellular heparinlike substance mediated adsorption of pseudorabies virus. Journal of Virology 64:278–286
    [Google Scholar]
  19. Okazaki K., Honda E., Minetoma T., Kumagai T. 1986; Mechanisms of neutralization by monoclonal antibodies to different antigenic sites on the bovine herpesvirus type 1 glycoproteins. Virology 150:260–264
    [Google Scholar]
  20. Okazaki K., Kawakura H., Okada M., Honda E., Minetoma T., Kumagai T. 1987; Intracellular localization of three different bovine herpesvirus type 1 glycoproteins involved in neutralization. Archives of Virology 92:17–26
    [Google Scholar]
  21. Okazaki K., Matsuzaki T., Sugahara Y., Okada J., Hasebe M., Iwamura Y., Ohnishi M., Kanno T., Shimizu M., Honda E., Kono Y. 1991; BHV-1 adsorption is mediated by the interaction of glycoprotein gIII with heparinlike moiety of the cell surface. Virology 181:666–670
    [Google Scholar]
  22. Okazaki K., Kanno T., Kiriya S., Honda E., Kono Y. 1993; Hemadsorptive activity of transfected COS-7 cells expressing BHV-1 glycoprotein gIII. Virology 193:1024–1027
    [Google Scholar]
  23. Okazaki K., Honda E., Kono Y. 1994; Heparin-binding domain of bovid herpesvirus 1 glycoprotein gIII. Archives of Virology in press
    [Google Scholar]
  24. Ribbins A. K., Watson R. J., Whealy M. E., Hays W. W., Enquist L. W. 1986; Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C. Journal of Virology 58:339–347
    [Google Scholar]
  25. Shing Y., Folkman J., Sullivan R., Butterfield C., Murry J., Klagsbrun M. 1984; Heparin affinity purification of a tumor derived capillary endothelial cell growth factor. Science 223:1296–1299
    [Google Scholar]
  26. Summers M. D., Smith G. E. 1987 A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures College Station: Texas Agriculture Experimental Station;
    [Google Scholar]
  27. Swain M. A., Peet R. W., Galloway D. A. 1985; Characterization of the gene encoding herpes simplex virus type 2 glycoprotein C and comparison with the type 1 counterpart. Journal of Virology 53:561–569
    [Google Scholar]
  28. van Drunen Littel-van den Hurk S., van den Hurk J. V., Gilchrist J. E., Misra V., Babiuk L. A. 1984; Interactions of monoclonal antibodies and bovine herpesvirus 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology 135:466–479
    [Google Scholar]
  29. van Drunen Littel-van den Hurk S., Parker M. D., Fitzpatrick D. R., van den Hurk J. V., Campos M., Babiuk L. A., Zamb T. 1992; Structural, functional, and immunological characterization of bovine herpesvirus 1 glycoprotein gI expressed by recombinant baculovirus. Virology 190:378–392
    [Google Scholar]
  30. Yamada K. M., Kennedy D. W., Kimata K., Pratt R. M. 1980; Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. Journal of Biological Chemistry 255:6055–6063
    [Google Scholar]
  31. Yayon A., Klagsbrun M., Esko J. D., Leder P., Ornitz D. M. 1991; Cell surface heparin-like molecules are required for binding of fibroblast growth factor to its high affinity receptor. Cell 64:841–848
    [Google Scholar]
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