Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus and 5′ genes Free

Abstract

Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gene (VGag) or plus the 5′ encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.

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1994-04-01
2024-03-29
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