1887

Abstract

Hybridomas secreting monoclonal antibodies (MAbs) specific for the surface protein (SU) of feline immunodeficiency virus were generated. Four MAbs were obtained which could be assigned to two groups based on their neutralization and competition behaviour. Using SU protein fragments expressed in the antigenic site recognized by one of the MAbs (2H11) could be mapped to the c terminus. The neutralizing MAb 1E1 did not bind to any of the SU protein fragments and was directed to a conformational epitope. Binding of the MAb 1E1 to native SU protein could be blocked with a rabbit serum raised against the SU3 fragment (amino acids 361 to 445). These data indicate that at least part of the epitope is located on this SU3 domain. In competition experiments most sera of naturally infected cats were able to inhibit binding of the MAbs. This shows the conserved and immunodominant nature of the epitopes involved.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-4-889
1994-04-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/4/JV0750040889.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-4-889&mimeType=html&fmt=ahah

References

  1. Ackley C. D., Yamamoto J. K., Levy N., Pedersen N. C., Cooper M. D. 1990; Immunologic abnormalities in pathogen-free cats experimentally infected with feline immunodeficiency virus. Journal of Virology 64:5652–5655
    [Google Scholar]
  2. Avrameas A., Guillet J.-G., Chouchane L., Moraillon A., Sonigo P., Strosberg A. D. 1992; Localisation of three epitopes of the env protein of feline immunodeficiency virus. Molecular Immunology 29:565–572
    [Google Scholar]
  3. Barlough J. E., Ackley C. D., George J. W., Levy N., Acevedo R., Moore P. F., Rideout B. A., Cooper M. D., Pedersen N. C. 1991; Acquired immune dysfunction in cats with experimentally induced feline immunodeficiency virus infection: comparison of short-term and long-term infections. Journal of Acquired Immune Deficiency Syndromes 4:219–227
    [Google Scholar]
  4. Berkower I., Smith G. E., Giri C., Murphey D. 1989; Human immunodeficiency virus 1: predominance of a group specific neutralizing epitope that persists despite genetic variation. Journal of Experimental Medicine 170:1681–1695
    [Google Scholar]
  5. Chiang C. S., Grove T., Cooper M., Cuan J., Kowalski A., Parcells K., Tsunokawa M., Rosenberg M., Arcuri E., Franklin S., Smith T., Debouck C. 1989; Development of a confirmatory enzyme-linked immunosorbent assay for HIV-1 antibodies. Clinical Chemistry 35:946–952
    [Google Scholar]
  6. De Ronde A, Boers P., Langedijk H., Meloen R., Hesselink W., Keldermans C. E. J. M., van Vliet A., Verschoor E. J., Horzinek M. C., Egberink H. F. 1994; Antibody response in cats to the envelope proteins of feline immunodeficiency virus: identification of an immunodominant neutralization domain. Virology 198:257–264
    [Google Scholar]
  7. Egberink H. F., Borst M., Niphuis H., Balzarini J., Neu H., Schellekens H., De Clercq E., Horzinek M., Koolen M. 1990a; Suppression of feline immunodeficiency virus infection in vivo by 9-(2-phosphonomethoxyethyl)adenine. Proceedings of the National Academy of Sciences, U.S A 87:3087–3091
    [Google Scholar]
  8. Egberink H. F., Ederveen J., Montelaro R. C., Pedersen N. C., Horzinek M. C., Koolen M. J. M. 1990b; Intracellular proteins of feline immunodeficiency virus and their antigenic relationship with equine infectious anaemia virus proteins. Journal of General Virology 71:739–743
    [Google Scholar]
  9. Egberink H. F., Keldermans C. J. E. M., Koolen M. J. M., Horzinek M. C. 1992; Humoral immune response to feline immunodeficiency virus in cats with experimentally induced and naturally acquired infections. American Journal of Veterinary Research 53:1133–1138
    [Google Scholar]
  10. Ishida T., Tomoda I. 1990; Clinical staging of feline immunodeficiency virus infection. Japanese Journal of Veterinary Science 52:645–648
    [Google Scholar]
  11. Jarrett O., Yamamoto J. K., Neil J. C. 1990; Feline immunodeficiency virus as a model for AIDS vaccination. AIDS 4: supplement 1 S163–S165
    [Google Scholar]
  12. Krone W. J. A., Debouck C., Epstein I. G., Heutink P., Meloen R., Goudsmit J. 1988; Natural antibodies to HIV-tat epitopes and expression of HIV-1 genes in vivo. Journal of Medical Virology 26:261–270
    [Google Scholar]
  13. Lombardi S., Garcelli C., La Rosa C., Zaccaro L., Specter S., Malvaldi G., Tozzini F., Esposito F., Bendinelli M. 1993; Identification of a linear neutralization site within the third variable region of the feline immunodeficiency virus envelope. Journal of Virology 67:4742–4749
    [Google Scholar]
  14. Palker T. J., Matthews T. J., Clark M. E., Cianciolo G. J., Randall R. R., Langlois A. J., White G. C., Safai B., Snyderman R., Bolognesi D. P., Haynes B. F. 1987; A conserved region at the COOH terminus of human immunodeficiency virus gpl20 envelope protein contains an immunodominant epitope. Proceedings of the National Academy of Sciences, U.S.A 84:2479–2483
    [Google Scholar]
  15. Pedersen N. C., Ho E. W., Brown M. L., Yamamoto J. K. 1987; Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science 235:790–793
    [Google Scholar]
  16. Prince A. M., Pascual D., Kosolapov L. B., Kurokawa D., Baker L., Rubinstein P. 1987; Prevalence, clinical significance, and strain specificity of neutralizing antibody to the human immunodeficiency virus. Journal of Infectious Diseases 156:268–272
    [Google Scholar]
  17. Rigby M. A., Holmes E. C., Pistello M., Mackay A., Leighbrown A. J., Neil J. C. 1993; Evolution of structural proteins of feline immunodeficiency virus: molecular epidemiology and evidence of selection for change. Journal of General Virology 74:425–436
    [Google Scholar]
  18. Robinson J. E., Holton D., Liu J., Mcmurpo H., Murciano A., Gohd R. 1990; A novel enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to HIV-1 envelope glycoproteins based on immobilization of viral glycoproteins in microtiter plates. Journal of Immunological Methods 132:63–71
    [Google Scholar]
  19. Siebelink K. H. J., Chu I., Rimmelzwaan G. F., Weijer K., Van Herwijnen R., Knell P., Egberink H. F., Bosch M. L., Osterhaus A. D. M. E. 1990; Feline immunodeficiency virus (FIV) infection in the cat as a model for HIV infection in man: FIV-induced impairment of immune function. AIDS Research and Human Retroviruses 6:1373–1378
    [Google Scholar]
  20. Tilton G. K., O’Connor T. P. Jr Seymour C. L., Lawrence K. L., Cohen N. D., Andersen P. R., Tonelli Q. J. 1990; Immunoassay for detection of feline immunodeficiency virus core antigen. Journal of Clinical Microbiology 28:898–904
    [Google Scholar]
  21. Tozzini F., Matteucci D., Bandecchi P., Baldinotti F., Siebelink K., Osterhaus A., Bendinelli M. 1993; Neutralizing antibodies in cats infected with feline immunodeficiency virus. Journal of Clinical Microbiology 31:1626–1629
    [Google Scholar]
  22. Weiss R., Clapham P., Weber J., Dalgleish A., Laskey L., Berman P. 1986; Variable and conserved neutralization antigens of the human immunodeficiency virus (HIV-1). Nature; London: 324572–595
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-4-889
Loading
/content/journal/jgv/10.1099/0022-1317-75-4-889
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error