Phagocytosis of modulates human immunodeficiency virus replication in human monocytic cells Free

Abstract

Macrophage activation resulting from phagocytosis has the potential to modulate human immunodeficiency virus (HIV) replication. We have determined the effects of phagocytosis of particulate stimuli on transcription and release of HIV. Using THP-1 and Mono Mac 6 human monocytic cell lines transfected with HIV long terminal repeat sequence chloramphenicol acetytransferase (LTR CAT) constructs we have demonstrated that phagocytosis of enhanced HIV-1 and -2 LTR CAT expression. However phagocytosis of zymosan or inert latex beads had little or no effect on CAT expression. Enhancement of HIV LTR CAT expression was dependent upon intact NF-κB binding sites and was independent of tumour necrosis factor alpha secretion. strains of different degrees of virulence induced similar levels of enhanced CAT expression. In contrast, phagocytosis of by HIV-1-infected THP-1 cells reduced supernatant reverse transcriptase (RT) activity without suppression of p24 antigen release. Phagocytosis of zymosan granules or latex particles did not alter released RT activity. However, phagocytosis of either , zymosan granules or latex particles by HIV-1-infected human peripheral blood monocyte-derived macrophages reduced supernatant RT activity. These data indicate that phagocytosis of may enhance HIV transcription in monocytic cells although it may reduce release of intact HIV.

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1994-04-01
2024-03-28
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References

  1. Allen B. W. A. 1969; Mycobacterium tuberculosis strain H37Rv. Journal of Medical Laboratory Technology 26:389–390
    [Google Scholar]
  2. Barnes P. F., Bloch A. B., Davidson P. T., Snider D. E. 1991; Tuberculosis m patients with human immunodeficiency virus infection. New England Journal of Medicine 324:1644–1650
    [Google Scholar]
  3. Biswas P., Poli G., Kinter A. L., Justement J. S., Stanley S. K., Maury W. J., Bressler P., Orenstein J. M., Fauci A. S. 1992; Interferon γ induces the expression of human immunodeficiency virus in persistently infected promonocytic cells (U1) and redirects the production of virions to intracytoplasmic vacuoles in phorbol myristate acetate differentiated U1 cells. Journal of Experimental Medicine 176:739–750
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Clouse K. A., Powell D., Washington I., Pole G., Strebbl K., Farrar W., Barstod P., Kovacs J., Fauci A., Folks T. M. 1989; Monokine regulation of human immunodeficiency virus-1 expression in chronically infected human T cell clone. Journal of Immunology 142:431–438
    [Google Scholar]
  6. Centers for Disease Control 1987; Revision of the CDC case surveillance definition for acquired immunodeficiency syndrome. Morbidity and Mortality Weekly Report 36 supplement 1S 3S–14S
    [Google Scholar]
  7. Cullen B. R., Greene W. C. 1989; Regulatory pathways governing HIV-1 replication. Cell 58:423–426
    [Google Scholar]
  8. Elliot A. M., Luo N., Tembo G., Halwiindi B., Steenbergen G., Machiels L., Pobee J., Nunn P., Hayes R. J., Mcadam K. P. W. J. 1990; Impact of HIV on tuberculosis in Zambia: a cross sectional study. British Medical Journal 301:412–415
    [Google Scholar]
  9. Espevik T., Nissen-Meyer J. 1986; A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. Journal of Immunological Methods 95:99–105
    [Google Scholar]
  10. Fernie B. F., Poli G., Fauci A. S. 1991; Alpha interferon suppresses virion but not soluble human immunodeficiency virus antigen production in chronically infected T-lymphocytic cells. Journal of Virology 65:3968–3971
    [Google Scholar]
  11. Ferns R. B., Tedder R. S., Weiss R. A. 1987; Characterization of monoclonal antibodies against the human immunodeficiency virus (HIV) gag products and their use in monitoring HIV isolate variation. Journal of General Virology 68:1543–1551
    [Google Scholar]
  12. Friedland J. S., Remick D. G., Shattock R. J., Griffin G. E. 1992; Secretion of interleukin-8 following phagocytosis of Mycobacterium tuberculosis by human monocyte cell lines. European Journal of Immunology 22:1373–1378
    [Google Scholar]
  13. Gartner A., Markovits P., Markovits D. M., Kaplan K. M., Gallo R. C., Popovic M. 1986; The role of mononuclear phagocytes in HTLV 3/LAV infection. Science 233:215–218
    [Google Scholar]
  14. Gendelman M. E., Orenstein J. M., Martin M. A., Ferrua C., Mitra F., Phipps T., Wahl L. A., Lane M. C., Fauci A. S., Burke D. S., Stillman D., Meltzer M. S. 1988; Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1 treated monocytes. Journal of Experimental Medicine 167:1428–1441
    [Google Scholar]
  15. Gendelman M. E., Orenstein J. M., Baca L. M., Weiser B., Burger H., Kalter D. C., Meltzer M. S. 1989; The macrophage in the persistence and pathogenesis of HIV infection. AIDS 3:475–495
    [Google Scholar]
  16. Gendelman H. E., Baca L., Turpin J. A., Kalter C., Hansen B. D., Orenstein J. M., Friedman R. M., Meltzer M. S. 1990; Restriction of HIV replication in infected T cells and monocytes by interferon alpha. AIDS Research and Human Retroviruses 6:1045–1049
    [Google Scholar]
  17. Ghosh S., Baltimore D. 1990; Activation in vitro of NF-AB by phosphorylation of its inhibitor 1kB. Nature; London: 344678–682
    [Google Scholar]
  18. Gorman C. M., Moffat L F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  19. Griffin G. E., Leung K., Folks T. M., Kunkel S., Nabel G. J. 1989; Activation of HIV gene expression during monocyte differentiation by induction of NF-kB. Nature; London: 33970–73
    [Google Scholar]
  20. Horsburgh C. R. 1991; Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. New England Journal of Medicine 324:1332–1338
    [Google Scholar]
  21. Innocenti P., Seigneurin J. M. 1990; HIV-2 chronic infection of promonocytic cells. Research in Virology 141:267–278
    [Google Scholar]
  22. Jeffrey A. A., Israel-Biet D., Andrieu J.-M., Even P., Venet A. 1991; HIV isolation from pulmonary cells derived from broncho-alveolar lavage. Clinical and Experimental Immunology 85:488–492
    [Google Scholar]
  23. Jones B. M., Nicholson J. K. A., Holman R. C., Hubbard M. 1989; Comparison of monocyte separation methods using flow cytometric analysis. Journal of Immunological Methods 125:41–47
    [Google Scholar]
  24. Koenig S., Gendelman H. E., Orenstein J. M., Canto M. C. D., Peseshkpour G. M., Yungbluth M., Jarrota F., Aksamit A., Martin M. A., Fausi A. S. 1986; Detection of AIDS virus in macrophages in bram tissue from AIDS patients with encephalopathy. Science 223:1089–1093
    [Google Scholar]
  25. Leonard J., Killan J. S., Gendelman H. E. 1989; The human immunodeficiency virus long terminal repeat is preferentially expressed in Langerhan cells in transgenic mice. AIDS Research and Human Retroviruses 5:421–430
    [Google Scholar]
  26. Markovitz D. M., Hannibal M., Perez V. Z., Gauntt C., Folks T. M., Nabel G. J. 1990; Differential regulation of human immunodeficiency viruses (HIVs): a specific regulatory element in HIV-2 responds to stimulation of the T cell antigen receptor. Proceedings of the National Academy of Sciences, U.S.A 87:9098–9102
    [Google Scholar]
  27. Meylan P. R. A., Munis J. R., Richman D. D., Kornbluth R. S. 1992; Concurrent human immunodeficiency virus and mycobacterial infection of macrophages in vitro does not reveal any reciprocal effect. Journal of Infectious Diseases 165:80–86
    [Google Scholar]
  28. Mikovits J. A., Lohrey N. C., Schulof R., Courtless J., Ruscetti F. W. 1992; Activation of infectious virus from latent human immunodeficiency virus infection of monocytes in vivo. Journal of Clinical Investigation 90:1486–1491
    [Google Scholar]
  29. Nabel G. J., Baltimore D. 1987; Inducible transcription factor promotes expression of human immunodeficiency virus in T cells. Nature; London: 326711–713
    [Google Scholar]
  30. Nottet H. S. L. M., Graaf L., de Vos N. M., Barker L. J., Strijp J. A. G., Visser M. R., Verhoef J. 1993; Down-regulation of human immunodeficiency virus type 1 (HIV-1) production after stimulation of monocyte-derived macrophages infected with HIV-1. Journal of Infectious Diseases 167:810–817
    [Google Scholar]
  31. Osborn L., Kunkel S., Nabel G. J. 1989; Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kB. Proceedings of the National Academy of Sciences, U.S.A 86:2336–2340
    [Google Scholar]
  32. Perkin N., Schmid R. M., Duckett C. S., Leung K., Rice N. R., Nabel G. J. 1992; Distinct combinations of NF-kB subunits determine the specificity of transcriptional activation. Proceeding of the National Academy of Sciences, U.S.A 89:1529–1533
    [Google Scholar]
  33. Pomerantz R. J., Feinberg H. B., Trono D., Baltimore D. 1990; Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type-1 ex-pression. Journal of Experimental Medicine 172:253–261
    [Google Scholar]
  34. Potts B. J. 1990; Mini reverse transcriptase (RT) assay. In Techniques in HIV Research pp. 103–106 Aldovini A., Walker B. Edited by New York: Stockton Press;
    [Google Scholar]
  35. Raziuddin J., Mikovits A., Calvert I., Ghosh S., Kung H.-F., Ruscetti F. W. 1991; Negative regulation of human immuno-deficiency virus type 1 expression in monocytes: role of the 65-kDa plus 50-kDa NF-xB dimer. Proceeding of the National Academy of Sciences, U.S.A 88:9426–9430
    [Google Scholar]
  36. Rosen C. A., Sodroski J. G., Campbell K., Haseltine W. A. 1986; Construction of recombinant murine retroviruses that express the human T-cell leukemia virus type II and human T-cell lymphotropic virus type III trans activator genes. Journal of Virology 57:379–384
    [Google Scholar]
  37. Shattock R. J., Friedland J. S., Griffin G. E. 1993; Release of human immunodeficiency virus by THP-1 cells and human macro-phages is regulated by cellular adherence and state of activation. Journal of Virology 67:3569–3575
    [Google Scholar]
  38. Tong-Starksen S. E., Welsh T. M., Peterlin B. M. 1990; Differences in transcriptional enhancers of HIV-1 and HIV-2, response to T cell activation signals. Journal of Immunology 145:4348–4354
    [Google Scholar]
  39. Tsuchiya S., Yamabe M., Yamaguchi Y., Kobayashi Y., Konno T., Tada K. 1980; Establishment and characterisation of a human acute monocytic cell line (THP-1). International Journal of Cancer 26:171–176
    [Google Scholar]
  40. Vlach J., Pitha P. M. 1992; Activation of human immuno-deficiency virus type 1 provirus in T cells and macrophages is associated with induction of inducer-specific NF-kB binding proteins. Virology 187:63–72
    [Google Scholar]
  41. Ziegler-Heitbrock H. W. L., Thiel E., Herzog V., Wirtz A., Riethmuller G. 1988; Establishment of a human cell line (Mono Mac 6) with characteristics of mature monocytes. International Journal of Cancer 41:456–461
    [Google Scholar]
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