@article{mbs:/content/journal/jgv/10.1099/0022-1317-75-4-819, author = "Segev, Y. and Rager-Zisman, B. and Isakov, N. and Schneider-Schaulies, S. and ter Meulen, V. and Udem, S. and Segal, S. and Wolfson, M.", title = "Reversal of the measles virus-mediated increase of phosphorylating activity in persistently infected mouse neuroblastoma cells by anti-measles virus antibodies", journal= "Journal of General Virology", year = "1994", volume = "75", number = "4", pages = "819-827", doi = "https://doi.org/10.1099/0022-1317-75-4-819", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-4-819", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "To investigate the effect of persistent measles virus infection on signal transduction in cells of neuronal origin, the mouse neuroblastoma cell line NS20Y/MS, which is persistently infected with measles virus, was used. The results demonstrate an approximate 50% increase in total phosphorylation and a similar increase in protein kinase C (PKC) activity. Western blot analysis with anti-total PKC or anti-PKC-α antibodies revealed a significant increase in the level of an 80K immuno-reactive PKC in NS20Y/MS cells. Following incubation of NS20Y/MS cells with polyclonal anti-measles virus antibodies, which down-regulate the level of measles virus proteins, total and PKC-mediated phosphorylation returned to the basal level of uninfected cells. This effect was reversible and removal of the antibodies resulted in restoration of the high level of total and PKC-mediated phosphorylation. The release of infectious measles virus was strongly inhibited by incubation of NS20Y/MS cells with the PKC inhibitor, 1-(5-isoquinolinylsulphonyl)-2-methylpiperazine (H-7). These results demonstrate that measles virus induces elevation in cellular phosphorylation which is essential for measles virus production.", }