1887

Abstract

The DNA sequence of herpes simplex virus type 1 (HSV-1) strain 17 in the region coding for the polypeptide ICP34.5 predicts a protein of 248 amino acids with a proposed of 26158. The entire RL1 open reading frame was cloned into the expression vector pET8c to enable over-expression of ICP34.5 in . The expressed protein was partially purified and used as an immunogen to produce a polyclonal antiserum in rabbits. Construction of an ICP34.5 null mutant (1771), demonstrated that the predicted open reading frame for ICP34.5 in strain 17 is correct and confirmed that HSV-1 strain 17 ICP34.5 specifically determines neurovirulence. The specificity of the antiserum directed against the -expressed ICP34.5 was defined by Western blotting of wild-type and RL1-negative infected cell extracts.

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/content/journal/jgv/10.1099/0022-1317-75-4-733
1994-04-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-4-733
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