1887

Abstract

We have synthesized and mapped a library of cDNA clones representing the RNA genome of a strain of blueberry scorch carlavirus (BBScY) associated with a disease known locally, in New Jersey, U.S.A., as Sheep Pen Hill disease. The nucleotide sequence of that strain was determined to be 8514 residues, excluding the poly(A) tail. In addition, cDNA clones representing the 3′ terminus of another strain of the virus from the same field were synthesized, mapped and sequenced. The overall identity between sequences of these two strains was approximately 90% spanning the 1634 residue overlap, confirming their identity as distinct strains and not simply different isolates of a single strain. Finally, the coat protein gene of a distinct strain of the virus, isolated from plants with blueberry scorch disease in the Puyallup Valley in Washington State, U.S.A., was cloned from total cDNA by PCR. Sequence analysis revealed that the strain from Washington was more divergent from the two New Jersey strains than they were from each other. Comparisons of these sequences with other carlavirus sequences indicated that BBScV is more closely related to lily symptomless virus and potato virus S than to potato virus M, virus S, carnation latent virus or poplar mosaic virus. BBScV and potato virus M shared approximately 54 % nucleotide sequence identity overall.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-4-711
1994-04-01
2022-05-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/4/JV0750040711.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-4-711&mimeType=html&fmt=ahah

References

  1. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of nucleotide sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  2. Dinesh-Kumar S. P., Brault V., Miller W. A. 1992; Precise mapping and in vitro translation of a trifunctional subgenomic RNA of barley yellow dwarf virus. Virology 187:711–722
    [Google Scholar]
  3. Felsenstein J. 1989; PHYLIP - phylogeny inference package (version 3.4). Cladistics 5:164–166
    [Google Scholar]
  4. Foster G. D., Mills P. R. 1990; Evidence for the role of subgenomic RNAs in the production of potato virus S coat protein during in vitro translation. Journal of General Virology 71:1247–1249
    [Google Scholar]
  5. Foster G. D., Mills P. R. 1991; Occurrence of chloroplast ribosome recognition sites within conserved elements of the RNA genomes of carlaviruses. FEES Letters 280:341–343
    [Google Scholar]
  6. Foster G. D., Mills P. R. 1992a; Translation of potato virus S RNA in vitro : Evidence for protein processing. Virus Genes 6:47–52
    [Google Scholar]
  7. Foster G. D., Mills P. R. 1992b; The 3′-nucleotide sequence of an ordinary strain of potato virus S. Virus Genes 6:213–220
    [Google Scholar]
  8. Foster G. D., Meehan B. M., Mills P. R. 1990a; A comparison of the nucleotide sequence homologies between isolates of the Andean and ordinary strains of potato virus S and their relationship to other carlaviruses. Virus Genes 4:257–260
    [Google Scholar]
  9. Foster G. D., Meehan B. M., Mills P. R. 1990b; Nucleotide sequence of the 7K gene of carnation latent virus. Plant Molecular Biology 15:937–939
    [Google Scholar]
  10. Foster G. D., Millar A. W., Meehan B. M., Mills P. R. 1990c; Nucleotide sequence of the 3′-terminal region of Helenium virus S RNA. Journal of General Virology 71:1877–1880
    [Google Scholar]
  11. Gilmer D., Bouzoubaa S., Hehn A., Guilley H., Richards K., Jonard G. 1992; Efficient cell-to-cell movement of beet necrotic yellow vein virus requires 3′ proximal genes located on RNA2. Virology 189:40–47
    [Google Scholar]
  12. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1989; Coronavirus genome: tentative functional mapping of the non-structural polyprotein by theoretical analysis of the amino acid sequence. Nucleic Acids Research 17:4456–4469
    [Google Scholar]
  13. Gramstat A., Courpozanis A., Rhode W. 1990; The 12 kDa protein of potato virus M displays properties of a nucleic acidbinding regulatory protein. FEBS Letters 276:34–38
    [Google Scholar]
  14. Haylor M. T. M., Brunt A. A., Coutts R. H. A. 1990; Conservation of the 3′ terminal nucleotide sequence in five carlaviruses. Nucleic Acids Research 18:6127
    [Google Scholar]
  15. Henderson J., Gibbs M. J., Edwards M. L., Clarke V. A., Gardner K. A., Cooper J. I. 1992; Partial nucleotide sequence of poplar mosaic virus RNA confirms its classification as a carlavirus. Journal of General Virology 73:1887–1890
    [Google Scholar]
  16. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  17. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignments. CABIOS 8:189–191
    [Google Scholar]
  18. Hillman B. I., Morris T. J., Schlegel D. E. 1985; Effects of low- molecular-weight RNA and temperature on tomato bushy stunt virus symptom expression. Phytopathology 75:361–365
    [Google Scholar]
  19. Hillman B. I., Tian Y., Bedker P. J., Brown M. P. 1992; A North American hypovirulent isolate of the chestnut blight fungus with European isolate-related dsRNA. Journal of General Virology 73:681–686
    [Google Scholar]
  20. Klug A., Rhodes D. 1987; ‘Zinc fingers’: a novel protein motif for nucleic acid recognition. TIBS 12:464–469
    [Google Scholar]
  21. Koonin E. V., Boyko V. P., Dolja V. V. 1991; Small cysteine- rich proteins of different groups of plant RNA viruses are related to different families of nucleic acid-binding proteins. Virology 181:395–398
    [Google Scholar]
  22. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
    [Google Scholar]
  23. Levay K., Zavriev S. 1991; Nucleotide sequence and gene organization of the 3ʹ-terminal region of chrysanthemum virus B genomic RNA. Journal of General Virology 72:2333–2337
    [Google Scholar]
  24. MacDonald S. G., Martin R. R., Bristow P. R. 1991; Characterization of an llarvirus associated with a necrotic shock reaction in blueberry. Phytopathology 81:210–214
    [Google Scholar]
  25. Mackenzie D. J., Tremaine J. H., Stace-Smith R. 1989; Organization and interviral homologies of the 3′-terminal portion of potato virus S RNA. Journal of General Virology 70:1053–1063
    [Google Scholar]
  26. Martin R. R., Bristow P. R. 1987; A virus associated with a new blight disease of highbush blueberry. Phytopathology 77:1721–1722
    [Google Scholar]
  27. Martin R. R., Bristow P. R. 1988; A carlavirus associated with blueberry scorch disease. Phytopathology 78:1636–1640
    [Google Scholar]
  28. Martin R. R., MacDonald S. G., Podleckis E. V. 1992; Relationships between blueberry scorch and Sheep Pen Hill viruses of highbush blueberry. Acta horticulturae 308:131–138
    [Google Scholar]
  29. Meehan B. M., Mills P. R. 1991; Nucleotide sequence of the 3′- terminal region of carnation latent virus. Intervirology 32:262–267
    [Google Scholar]
  30. Memelink J., Van Der Vlugt C. I. M., Linthorst H. J. M., Derks A.F.L.M., Asjes C. J., Bol J. F. 1990; Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants. Journal of General Virology 71:917–924
    [Google Scholar]
  31. Monis J., De Zoeten G. A. 1990; Molecular cloning and physical mapping of potato virus S complementary DNA. Phytopathology 80:446–450
    [Google Scholar]
  32. Morozov S. YU., Miroshnichenko N. A., Zelenina D. A., Fedorkin O. N., Solovijev A. G., Lukasheva L. I., Atabekov J. C. 1990a; Expression of RNA transcriptions of potato virus X full-length and subgenomic cDNAs. Biochimie 72:677–684
    [Google Scholar]
  33. Morozov S. YU., Kanyuka K. V., Levay K. E., Zavriev S. K. 1990b; The putative replicase of potato virus M: obvious sequence similarity with potex- and tymoviruses. Virology 179:911–914
    [Google Scholar]
  34. Morozov S. Y., Miroshnichenko N. A., Solovyev A. G., Zelenina D. A., Fedorkin O. N., Lukasheva L. I., Grachev S. A., Chernov B. K. 1991; In vitro membrane binding of the translation products of the carlavirus 7-kDa protein genes. Virology 183:782–785
    [Google Scholar]
  35. Poch O., Sauvaget I., Delarue M., Tordo N. 1989; Identification of four motifs among the RNA-dependent polymerase encoding elements. EMBO Journal 12:3867–3874
    [Google Scholar]
  36. Podleckis E. V., Davis R. F. 1989; Infection of highbush blueberries with a putative carlavirus. Acta horticulturae 241:338–343
    [Google Scholar]
  37. Podleckis E. V., Davis R. F., Stretch A. W., Schulz C. P. 1986; Flexuous rod particles associated with Sheep Pen Hill Disease of highbush blueberries. Phytopathology 76:1065
    [Google Scholar]
  38. Queen C., Korn L. J. 1984; A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Research 12:581–599
    [Google Scholar]
  39. Rupasov V. V., Morozov S. YU., Kanyuka K. V., Zavriev S. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
    [Google Scholar]
  40. Ruther U., Müller-Hill B. 1983; Easy identification of cDNA clones. EMBO Journal 2:1791–1794
    [Google Scholar]
  41. Scheidel L. M., Durbin R. K., Stollar V. 1989; SVLM21, a Sindbis virus mutant resistant to methionine deprivation, encodes an altered methyltransferase. Virology 173:408–414
    [Google Scholar]
  42. Stretch A. W. 1983; A previously undescribed blight disease of highbush blueberries in New Jersey. Phytopathology 73:375
    [Google Scholar]
  43. Tavantzis S. M. 1991; Coat protein and protease activity as in vitro translation products of potato carlavirus M RNA. Archives of Virology 120:241–252
    [Google Scholar]
  44. Zavriev S. K., Kanyuka K. V., Levay K. E. 1991; The genome organization of potato virus M RNA. Journal of General Virology 72:9–14
    [Google Scholar]
  45. Zuidema D., Heaton L. A., Jackson A. O. 1987; Structure of the nucleocapsid gene of sonchus yellow net virus. Virology 159:373–380
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-4-711
Loading
/content/journal/jgv/10.1099/0022-1317-75-4-711
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error