1887

Abstract

Bunyaviruses have a genome comprising three segments of negative-sense RNA. The smallest RNA segment, S, encodes the nucleocapsid protein, N, and a nonstructural protein, NSs, in overlapping reading frames. The sequences of the S genome RNA segments of seven bunyaviruses (Batai, Cache Valley, Guaroa, Kairi, Main Drain, Northway and Lumbo) were determined from cloned cDNAs obtained using a one-step reverse transcription-PCR protocol. These sequences were compared to those of six viruses previously published, reinforcing earlier conclusions about relationships of the bunyaviruses. Sequence homologies between N proteins correlated with the subdivision of these viruses into three serogroups, Bunyamwera, California and Simbu.

The encoded N proteins are either 233 or 235 amino acids in length, depending on the serogroup, whereas the NSs proteins are more variable (83 to 109 amino acids). Certain nucleotide sequence motifs are conserved in the S segments of the Bunyamwera and California serogroup viruses, including the spacing of the AUG initiation codons for the N and NSs proteins (except Guaroa virus), and a CA-rich motif in the virion-sense RNA just downstream of the predicted mRNA termination site. A duplicated sequence was observed in the 3′non-coding region of the Lumbo virus S segment, which accounts for the significantly longer S genome segment of this virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-3-597
1994-03-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/3/JV0750030597.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-3-597&mimeType=html&fmt=ahah

References

  1. Akashi H., Gay M., Ihara T., Bishop D. H. L. 1984; Localized conserved regions of the S RNA gene products of bunyaviruses are revealed by sequence analyses of the Simbu serogroup Aino virus. Virus Research 1:51–63
    [Google Scholar]
  2. Becerra S. P., Rose J. A., Hardy M., Baroudy B. M., Anderson C. W. 1985; Direct mapping of adeno-associated virus capsid proteins B and C: a possible ACG initiation codon. Proceedings of the National Academy of Sciences, U.S.A 82:7919–7923
    [Google Scholar]
  3. Bishop D. H. L. 1990; Bunyaviridae and their replication. I. Bunyaviridae. In Virology, 2nd edn. pp. 1155–1173 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  4. Bishop D. H. L., Shope R. E. 1979; Bunyaviridae. Comprehensive Virology 14:1–36
    [Google Scholar]
  5. Bishop D. H. L., Gould K. G., Akashi H., Clerx-van Haaster C. M. 1982; The complete sequence and coding content of snowshoe hare bunyavirus (S) viral RNA species. Nucleic Acids Research 10:3703–3713
    [Google Scholar]
  6. Bouloy M., Pardigon N., Vialat P., Gerbaud S., Girard M. 1990; Characterization of the 5′and 3′ends of viral messenger RNAs isolated from BHK21 cells infected with Germiston virus (Bunyavirus). Virology 75:50–58
    [Google Scholar]
  7. Cabradilla C. D., Holloway B. P., Obiieski J. F. 1983; Molecular cloning and sequencing of the La Crosse virus S RNA. Virology 128:463–468
    [Google Scholar]
  8. Calisher C. H. 1991; Bunyaviridae. Archives of Virology supplementum 2:273–283
    [Google Scholar]
  9. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  10. Curran J., Kolakofsky D. 1988; Ribosomal initiation from an ACG codon in the Sendai virus P/C mRNA. EMBO Journal 7:245–251
    [Google Scholar]
  11. Elliott R. M. 1989a; Nucleotide sequence analysis of the S RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae. Journal of General Virology 70:1281–1285
    [Google Scholar]
  12. Elliott R. M. 1989b; Nucleotide sequence analysis of the large (L) genomic RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae. Virology 173:426–436
    [Google Scholar]
  13. Elliott R. M. 1990; Molecular biology of the Bunyaviridae. Journal of General Virology 71:501–522
    [Google Scholar]
  14. Elliott R. M., Mcgregor A. 1989; Nucleotide sequence and expression of the small (S) RNA segment of Maguari bunyavirus. Virology 171:516–524
    [Google Scholar]
  15. Elliott R. M., Lees J. F., Watret G. E., Clark W., Pringle C. R. 1984; Genetic diversity of bunyaviruses and mechanisms of genetic variation. In Mechanisms of Viral Pathogenesis: from Gene to Pathogen pp. 61–76 Kohn A., Fuchs P. Edited by Boston: Martinus Nijholf;
    [Google Scholar]
  16. Elliott R. M., Schmaljohn C. S., Collett M. S. 1991; Bunyaviridae genome structure and gene expression. Current Topics in Microbiology and Immunology 169:91–141
    [Google Scholar]
  17. Eshita Y., Ericson B., Romanowski V., Bishop D. H. L. 1985; Analyses of the mRNA transcription processes of snowshoe hare bunyavirus S and M RNA species. Journal of Virologv 55:681–689
    [Google Scholar]
  18. Felsenstein J. 1991 PHYLIP Manual version 3.4 Berkeley: University Herbarium, University of California;
    [Google Scholar]
  19. Geliebter J., Zeff R. A., Melvold R. W., Nathanson S. G. 1986; Mitotic recombination in germ cells generated to major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis; Kbm9 and Kbm6. Proceedings of the National Academy of Sciences, U.S.A 83:3371–3375
    [Google Scholar]
  20. Gerbaud S., Vialet P., Pardigon N., Wychowski C., Girard M., Bouloy M. 1987; The S segment of Germiston virus RNA genome can code for three proteins. Virus Research 8:1–13
    [Google Scholar]
  21. Gupta K. C., Patwardhan S. 1988; ACG, the initiator codon for a Sendai virus protein. Journal of Biological Chemistry 263:8553–8556
    [Google Scholar]
  22. Hunt A. P., Calisher C. H. 1979; Relationships of Bunyamwera group viruses by neutralization. American Journal of Tropical Medicine and Hygiene 28:740–749
    [Google Scholar]
  23. Jin H., Elliott R. M. 1993; Characterization of Bunyamwera virus S RNA that is transcribed and replicated by the L protein expressed from recombinant vaccinia virus. Journal of Virology 67:1396–1404
    [Google Scholar]
  24. Lees J. F., Pringle C. R., Elliott R. M. 1986; Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the bunyavirus glycoprotein gene product. Virology 148:1–14
    [Google Scholar]
  25. Patterson J. L., Kolakofsky D. 1984; Characterization of La Crosse virus small genome transcripts. Journal of Virology 49:680–685
    [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  27. Schmaljohn C. S., Patterson J. L. 1990; Bunyaviridae and their replication. II. Replication of Bunyaviridae. In Virology, 2nd edn. pp. 1175–1194 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  28. Ushijima H., Klimas R., Kim S., Cash P., Bishop D. H. L. 1980; Characterization of the viral ribonucleic acids and structural polypeptides of Anopheles A, Bunyamwera, Group C, California, Capim, Guama, Patois and Simbu bunyaviruses. American Journal of Tropical Medicine and Hygiene 29:1441–1452
    [Google Scholar]
  29. Watret G. E., Pringle C. R., Elliott R. M. 1985; Synthesis of bunyavirus-specific proteins in a continuous cell line (XTC-2) derived from Xenopus laevis. Journal of General Virology 67:473–482
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-3-597
Loading
/content/journal/jgv/10.1099/0022-1317-75-3-597
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error