The complete genome of a Thailand isolate of the geminivirus tomato yellow leaf curl virus (TYLCV-Th) has been cloned and the nucleotide sequence determined. The genome consists of two DNAs each slightly greater than 2700 nucleotides in length and designated A-DNA and B-DNA. The A-DNA contains six open reading frames (ORFs) capable of encoding proteins with Mrs greater than 10K; two ORFs were located on the B-DNA. The predicted ORFs were found at positions on the genome similar to those identified in other geminiviruses. Based upon computer-assisted sequence comparisons with other geminiviruses, TYLCV-Th maintained the greatest degree of similarity with a tomato pathogen isolated in Australia, tomato leaf curl virus. Furthermore, sequence comparisons with a large number of geminiviruses confirm further divisions of the geminiviruses based upon geographical proximity of the viruses. Agroinoculation of Nicotiana benthamiana with a TYLCV-Th A-DNA clone containing a mutation in the capsid protein ORF demonstrated that the coat protein is not essential for TYLCV-Th infection but enhances the progression of the disease.
AbouzidA. M.,
PolstonJ. E.,
HiebertE.1992; The nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in Florida. Journal of General Virology 73:3225–3229
AndersenM. T.,
RichardsonK. A.,
HarrisonS.,
MorrisB. A. M.1988; Nucleotide sequence of the geminivirus Chloris striate mosaic virus. Virology 164:443–149
BoultonM. I.,
SteinkellnerH.,
DonsonJ.,
MarkhamP. G.,
KingD. I.,
DaviesJ. W.1989; Mutational analysis of the virion-sense genes of maize streak virus. Journal of General Virology 70:2309–2323
BroughC. L.,
HayesR. J.,
MorganA. J.,
CouttsR. H. A.,
BuckK. W.1988; Effects of mutagenesis in vitro on the ability of cloned tomato golden mosaic virus DNA to infect Nicotiana benthamiana plants. Journal of General Virology 69:503–514
DonsonJ.,
AccottoG. P.,
BoultonM. I.,
MullineauxP. M.,
DaviesJ. W.1987; The nucleotide sequence of a geminivirus from Digitaria sanguinalis
. Virology 161:160–169
DryI. B.,
RigdenJ. E.,
KrakeL. R.,
MullineauxP. M.,
RezaianM. A.1993; Nucleotide sequence and genome organization of tomato leaf curl geminivirus. Journal of General Virology 74:147–151
EtessamiP.,
WattsJ.,
StanleyJ.1989; Size reversion of African cassava mosaic virus coat protein gene deletion mutants during infection of Nicotiana benthamiana
. Journal of General Virology 70:277–289
FrischmuthT.,
ZimmatG.,
JeskeH.1990; The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–468
GardinerW. E.,
SunterG.,
BrandL.,
ElmerJ. S.,
RogersS. G.,
BisaroD. M.1988; Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread of symptom development. EMBO Journal 7:899–904
GilbertsonR. L.,
FariaJ. C.,
HansonS. F.,
MoralesF. J.,
AhlquistP.,
MaxwellD. P.,
RusselD. R.1991a; Cloning of the complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric particle discharge acceleration. Phytopathology 81:980–985
GilbertsonR. L.,
HidayatS. H.,
RojasM. R.,
MaxwellD. P.1991b; Infectious DNA clones of a new geminivirus associated with tomatoes in Florida. Phytopathology 81:1184
HamiltonW. D. O.,
BisaroD. M.,
CouttsR. H. A.,
BuckK. W.1983; Demonstration of the bipartite nature of the genome of a single-stranded plant virus by infection with cloned DNA components. Nucleic Acids Research 11:7387–7396
HamiltonW. D. O.,
SteinV.,
CouttsR. H. A.,
BuckK. W.1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
HowarthA. J.,
CatonJ.,
BossbrtM.,
GoodmanR. M.1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings of the National Academy of Sciences U.S.A: 823572–3576
HullR.,
StanleyJ.,
BriddonR. W.1991; The geminivirus group. In Classification and Nomenclature of Viruses
Archives of Virology Supplementum 2 pp 173–177FranckiR. I. B.,
FauquetC. M.,
KnudsonD. L.,
BrownF.
Edited by New York: Springer-Verlag;
KlinkenbergF. A.,
StanleyJ.1990; Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana
. Journal of General Virology 71:1409–1412
LazarowitzS. G.,
LazdinsI. N.1991; Infectivity and complete nucleotide sequence of the cloned genomic components of the bipartite squash leaf curl geminivirus with a broad host range phenotype. Virology 180:58–69
MacdowellS. W.,
MacdonaldH.,
HamiltonW. D. O.,
CouttsR. H. A.,
BuckK. W.1985; The nucleotide sequence of cloned wheat dwarf virus DNA. EMBO Journal 4:2173–2180
MullineauxP. M.,
DonsonJ.,
Morris-KrsinichB. A. M.,
BoultonM. I.,
DaviesJ. W.1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
NavotN.,
PicherskiE.,
ZeidanM.,
ZamirD.,
CzosnekH.1991; Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic molecule. Virology 185:151–161
RigdenJ. E.,
DryI. B.,
MullineauxP. M.,
RezaianM. A.1993; Mutagenesis of the virion-sense open reading frames of tomato leaf curl geminivirus. Virology 193:1001–1005
RochesterD. E.,
KositratanaW.,
BeachyR. N.1990; Systemic movement and symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand). Virology 178:520–526
RogersS. G.,
HorschR. B.,
FraleyR. T.1986; Gene transfer in plants: production of transformed plants using Ti plasmid vectors. Methods in Enzymology 118:627–640
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A: 745463–5467
StanleyJ.,
TownsendR.1986; Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA clones containing single copies of the genome. Nucleic Acids Research 14:5981–5998
StanleyJ.,
MarkhamP. G.,
CallisR. J.,
PinnerM. S.1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767