1887

Abstract

The complete genome of a Thailand isolate of the geminivirus tomato yellow leaf curl virus (TYLCV-Th) has been cloned and the nucleotide sequence determined. The genome consists of two DNAs each slightly greater than 2700 nucleotides in length and designated A-DNA and B-DNA. The A-DNA contains six open reading frames (ORFs) capable of encoding proteins with s greater than 10K; two ORFs were located on the B-DNA. The predicted ORFs were found at positions on the genome similar to those identified in other geminiviruses. Based upon computer-assisted sequence comparisons with other geminiviruses, TYLCV-Th maintained the greatest degree of similarity with a tomato pathogen isolated in Australia, tomato leaf curl virus. Furthermore, sequence comparisons with a large number of geminiviruses confirm further divisions of the geminiviruses based upon geographical proximity of the viruses. Agroinoculation of with a TYLCV-Th A-DNA clone containing a mutation in the capsid protein ORF demonstrated that the coat protein is not essential for TYLCV-Th infection but enhances the progression of the disease.

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1994-03-01
2021-10-25
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References

  1. Abouzid A. M., Polston J. E., Hiebert E. 1992; The nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in Florida. Journal of General Virology 73:3225–3229
    [Google Scholar]
  2. Andersen M. T., Richardson K. A., Harrison S., Morris B. A. M. 1988; Nucleotide sequence of the geminivirus Chloris striate mosaic virus. Virology 164:443–149
    [Google Scholar]
  3. Boulton M. I., Steinkellner H., Donson J., Markham P. G., King D. I., Davies J. W. 1989; Mutational analysis of the virion-sense genes of maize streak virus. Journal of General Virology 70:2309–2323
    [Google Scholar]
  4. Briddon R. W., Watts J., Markham P. G., Stanley J. 1989; The coat protein of beet curly top virus is essential for infectivity. Virology 172:628–633
    [Google Scholar]
  5. Brough C. L., Hayes R. J., Morgan A. J., Coutts R. H. A., Buck K. W. 1988; Effects of mutagenesis in vitro on the ability of cloned tomato golden mosaic virus DNA to infect Nicotiana benthamiana plants. Journal of General Virology 69:503–514
    [Google Scholar]
  6. Donson J., Accotto G. P., Boulton M. I., Mullineaux P. M., Davies J. W. 1987; The nucleotide sequence of a geminivirus from Digitaria sanguinalis . Virology 161:160–169
    [Google Scholar]
  7. Dry I. B., Rigden J. E., Krake L. R., Mullineaux P. M., Rezaian M. A. 1993; Nucleotide sequence and genome organization of tomato leaf curl geminivirus. Journal of General Virology 74:147–151
    [Google Scholar]
  8. Etessami P., Watts J., Stanley J. 1989; Size reversion of African cassava mosaic virus coat protein gene deletion mutants during infection of Nicotiana benthamiana . Journal of General Virology 70:277–289
    [Google Scholar]
  9. Frischmuth T., Zimmat G., Jeske H. 1990; The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–468
    [Google Scholar]
  10. Gardiner W. E., Sunter G., Brand L., Elmer J. S., Rogers S. G., Bisaro D. M. 1988; Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread of symptom development. EMBO Journal 7:899–904
    [Google Scholar]
  11. Gilbertson R. L., Faria J. C., Hanson S. F., Morales F. J., Ahlquist P., Maxwell D. P., Russel D. R. 1991a; Cloning of the complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric particle discharge acceleration. Phytopathology 81:980–985
    [Google Scholar]
  12. Gilbertson R. L., Hidayat S. H., Rojas M. R., Maxwell D. P. 1991b; Infectious DNA clones of a new geminivirus associated with tomatoes in Florida. Phytopathology 81:1184
    [Google Scholar]
  13. Hamilton W. D. O., Bisaro D. M., Coutts R. H. A., Buck K. W. 1983; Demonstration of the bipartite nature of the genome of a single-stranded plant virus by infection with cloned DNA components. Nucleic Acids Research 11:7387–7396
    [Google Scholar]
  14. Hamilton W. D. O., Stein V., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  15. Howarth A. J., Vandemark G. J. 1989; Phylogeny of geminiviruses. Journal of General Virology 70:2717–2727
    [Google Scholar]
  16. Howarth A. J., Caton J., Bossbrt M., Goodman R. M. 1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings of the National Academy of Sciences U.S.A: 823572–3576
    [Google Scholar]
  17. Hull R., Stanley J., Briddon R. W. 1991; The geminivirus group. In Classification and Nomenclature of Viruses Archives of Virology Supplementum 2 pp 173–177 Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. Edited by New York: Springer-Verlag;
    [Google Scholar]
  18. Kheyr-Pour A., Bendahmane M., Matzeit V., Accotto G. P., Crespi S., Gronenborn B. 1991; Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucleic Acids Research 19:6763–6769
    [Google Scholar]
  19. Klinkenberg F. A., Stanley J. 1990; Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana . Journal of General Virology 71:1409–1412
    [Google Scholar]
  20. Lazarowitz S. G., Lazdins I. N. 1991; Infectivity and complete nucleotide sequence of the cloned genomic components of the bipartite squash leaf curl geminivirus with a broad host range phenotype. Virology 180:58–69
    [Google Scholar]
  21. Lazarowitz S. G., Pinder A. J., Damsteegt V. D., Rogers S. G. 1989; Maize streak virus genes essential for systemic spread and symptom development. EMBO Journal 8:1023–1032
    [Google Scholar]
  22. Macdowell S. W., Macdonald H., Hamilton W. D. O., Coutts R. H. A., Buck K. W. 1985; The nucleotide sequence of cloned wheat dwarf virus DNA. EMBO Journal 4:2173–2180
    [Google Scholar]
  23. Mullineaux P. M., Donson J., Morris-Krsinich B. A. M., Boulton M. I., Davies J. W. 1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
    [Google Scholar]
  24. Navot N., Picherski E., Zeidan M., Zamir D., Czosnek H. 1991; Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic molecule. Virology 185:151–161
    [Google Scholar]
  25. Rigden J. E., Dry I. B., Mullineaux P. M., Rezaian M. A. 1993; Mutagenesis of the virion-sense open reading frames of tomato leaf curl geminivirus. Virology 193:1001–1005
    [Google Scholar]
  26. Rochester D. E., Kositratana W., Beachy R. N. 1990; Systemic movement and symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand). Virology 178:520–526
    [Google Scholar]
  27. Rogers S. G., Horsch R. B., Fraley R. T. 1986; Gene transfer in plants: production of transformed plants using Ti plasmid vectors. Methods in Enzymology 118:627–640
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A: 745463–5467
    [Google Scholar]
  29. Stanley J. 1983; Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature; London: 305643–645
    [Google Scholar]
  30. Stanley J., Gay M. R. 1983; Nucleotide sequence of cassava latent virus DNA. Nature; London: 301260–262
    [Google Scholar]
  31. Stanley J., Townsend R. 1986; Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA clones containing single copies of the genome. Nucleic Acids Research 14:5981–5998
    [Google Scholar]
  32. Stanley J., Markham P. G., Callis R. J., Pinner M. S. 1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
    [Google Scholar]
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