%0 Journal Article %A Puurand, ülo %A Mäkinen, Kristiina %A Paulin, Lars %A Saarma, Mart %T The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses %D 1994 %J Journal of General Virology, %V 75 %N 2 %P 457-461 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-75-2-457 %I Microbiology Society, %X The complete nucleotide sequence of potato virus A (PVA) was obtained from six independent cDNA clones. The RNA genome of PVA is 9565 nucleotides long and contains one open reading frame (ORF) of 9177 bases encoding a large polyprotein of 3059 amino acids with a calculated M r of 340K. Seven potential proteinase NIa, one HC-pro and one P1 proteinase recognition sites were found in PVA polyprotein by searching for cleavage site consensus sequences amongst the potyvirus group. The non-coding region preceding the ORF is 161 nucleotides long. The termination codon is followed by a 227-nucleotide sequence. Overall nucleotide sequence identity compared with several completely sequenced potyvirus genomes is between 53 and 58 %, with overall amino acid sequence identity between 65 and 71%. When the putative amino acid sequences of individual proteins of PVA were compared with the corresponding proteins of other potyviruses, P1 and P3 appeared the least conserved (34 to 53 %) whereas the other proteins were in most cases from 63 to 80% identical to each other. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-2-457