1887

Abstract

We have recovered five infectious molecular clones of the lentivirus equine infectious anaemia virus (EIAV). The clones were recovered from fetal equine kidney (FEK) cells infected with a virulent, cell culture-adapted virus stock (designated PV) and have been characterized at a molecular level. Each clone has unique envelope and long terminal repeat (LTR) sequences. We further investigated LTR sequence variation in the PV stock using PCR amplification to obtain additional LTR clones from infected FEK cells and from peripheral blood mononuclear cells (PBMCs) from animals experimentally infected with PV. Sequence analysis of resulting clones indicates a selection for different LTR populations in pony PBMCs compared to FEK cells. Finally, we observed that the cloned EIAV proviruses did not remain infectious when maintained in a derivative of pBR322. However, two proviruses have been stably maintained in a low copy number vector (PLG338-SPORT).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-2-425
1994-02-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/2/JV0750020425.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-2-425&mimeType=html&fmt=ahah

References

  1. Ball J. M., Rushlow K. E., Issel C. J., Montelaro R. C. 1992; Detailed mapping of the antigenicity of the surface unit glycoprotein of equine infectious anemia virus by using synthetic peptide strategies. Journal of Virology 66:732–742
    [Google Scholar]
  2. Benton C. V., Brown B. L., Harshman J. S., Gilden R. V. 1981; In vitro host range of equine infectious anemia virus. Intervirology 16:225–232
    [Google Scholar]
  3. Carpenter S., Chesebro B. 1989; Change in host cell tropism associated with in vitro replication of equine infectious anemia virus. Journal of Virology 63:2492–2496
    [Google Scholar]
  4. Carpenter S., Alexandersen S., Long M. J., Perryman S., Chesebro B. 1991; Identification of a hypervariable region in the long terminal repeat of equine infectious anemia virus. Journal of Virology 65:1605–1609
    [Google Scholar]
  5. Carvalho M., Derse D. 1993a; Physical and functional characterization of transcriptional control elements in the equine infectious anemia virus promotor. Journal of Virology 67:2064–2074
    [Google Scholar]
  6. Carvalho M., Derse D. 1993b; The PU.l/Spi-1 proto-oncogene is a transcriptional regulator of a lentivirus promotor. Journal of Virology 67:3885–3890
    [Google Scholar]
  7. Clabough D. L., Gebhard D., Flaherty M. T., Perry S. T., Coggins L., Fuller F. J. 1991; Immune-mediated thrombocytopenia in horses infected with equine infectious anemia virus. Journal of Virology 65:6242–6251
    [Google Scholar]
  8. Corboy J. R., Buzy J. M., Zink M. C., Clements J. E. 1992; Expression directed from HIV long terminal repeats in the central nervous system of transgenic mice. Science 258:1804–1808
    [Google Scholar]
  9. Cunningham T. P., Montelaro R. C., Rushlow K. E. 1993; Lentivirus envelope sequences and proviral genomes are stabilized in Escherichia coli when cloned in low-copy-number plasmid vectors. Gene 124:93–98
    [Google Scholar]
  10. Gregory R. J., Cheng S. H., Rich D. P., Marshall J., Paul S., Hehir K., Ostedgaard L., Klinger K. W., Welsh M. J., Smith A. E. 1990; Expression and characterization of the cystic fibrosis transmembrane conductance regulator. Nature; London: 347382–386
    [Google Scholar]
  11. Issel C. J., Coggins L. 1979; Equine infectious anemia: current knowledge. Journal of the American Veterinary Medical Association 174:727–733
    [Google Scholar]
  12. Issel C. J., Horohov D. W., Lea D. F., Adams W. V.Jr Hagius S. D., McManus J. M., Allison A. C., Montelaro R. C. 1992; Efficacy of inactivated whole-virus and subunit vaccines in preventing infection and disease caused by equine infectious anemia virus. Journal of Virology 66:3398–3408
    [Google Scholar]
  13. Kono Y., Yoshino T., Fukunaga Y. 1970; Growth characteristics of equine infectious anaemia virus in horse leukocyte cultures. Archiv für die Gesamte Virusforschung 30:252–256
    [Google Scholar]
  14. Orrego A., Issel C. J., Montelaro R. C., Adams W. V. 1982; Virulence and in vitro growth of a cell-adapted strain of equine infectious anemia virus after serial passage in ponies. American Journal of Veterinary Research 43:1556–1560
    [Google Scholar]
  15. Paquette Y., Kay D. G., Rassart E., Robitaille Y., Jolicoeur P. 1990; Substitution of the U3 long terminal repeat region of the neurotropic Cas-BR-E retrovirus affects its disease-inducing potential. Journal of Virology 64:3742–3752
    [Google Scholar]
  16. Payne S. L., Fang F., Liu C. P., Dhruva B. R., Rwambo P., Issel C. J., Montelaro R. C. 1987; Antigenic variation and lentivirus persistence: variations in envelope gene sequences during EIAV infection resemble changes reported for sequential isolates of HIV. Virology 161:321–331
    [Google Scholar]
  17. Perry S. T., Flaherty M. T., Kelley M. J., Clabough D. L., Tronick S. R., Coggins L., Whetter L., Lengel C. R., Fuller F. 1992; The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro. Journal of Virology 66:4085–4097
    [Google Scholar]
  18. Rasty S., Dhruva B. R., Schiltz L., Shih D. S., Issel C. J., Montelaro R. C. 1990; Proviral DNA integration and transcriptional patterns of equine infectious anemia virus during persistent and cytopathic infections. Journal of Virology 64:86–95
    [Google Scholar]
  19. Rushlow K., Olsen K., Stiegler G., Payne S. L., Montelaro R. C., Issel C. J. 1986; Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus. Virology 155:309–321
    [Google Scholar]
  20. Rwambo P. M., Issel C. J., Hussain K. A., Montelaro R. C. 1990; In vitro isolation of a neutralization escape mutant of equine infectious anaemia virus (EIAV). Archives of Virology 111:275–280
    [Google Scholar]
  21. Sellon D. C., Perry S. T., Coggins L., Fuller F. F. 1992; Wild-type equine infectious anemia virus replicates in vivo predominantly in tissue macrophages, not in peripheral blood monocytes. Journal of Virology 66:5906–5913
    [Google Scholar]
  22. Small J. A., Bieberich C., Ghotbi Z., Hess J., Scangos G. A., Clements J. E. 1989; The visna virus long terminal repeats direct expression of a reporter gene in activated macrophages, lymphocytes, and the central nervous system of transgenic mice. Journal of Virology 63:1891–1896
    [Google Scholar]
  23. Threadgill D. S., Steagall W. K., Flaherty M. T., Fuller F. F., Perry S. T., Rushlow K. E., LeGrice F. J., Payne S. L. 1993; Characterization of equine infectious anemia virus dUTPase: growth properties of a dUTPase-deficient mutant. Journal of Virology 67:2592–2600
    [Google Scholar]
  24. Whetter L., Archambouit D., Perry S., Gazit A., Coggins L., Yaniv A., Clabough D., Dahlberg J., Fuller F., Tronick S. 1990; Equine infectious anemia virus derived from a molecular clone persistently infects horses. Journal of Virology 64:5750–5756
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-2-425
Loading
/content/journal/jgv/10.1099/0022-1317-75-2-425
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error