1887

Abstract

We have compared the nucleotide sequence of an envelope protein gene fragment encoding amino acids 291 to 406 of 22 yellow fever (YF) virus strains of diverse geographic and host origins isolated over a 63 year time span. The nucleotide fragment of viral RNA was examined by direct sequencing of a PCR product derived from complementary DNA. Alignment with the prototype Asibi strain sequence showed divergence of 0 to 2·15 % corresponding to a maximum of 5·2 % divergence in the amino acid sequence. Taking 10% nucleotide divergence as a cut-off point, the 22 YF virus strains fell into three topotypes which corresponded to different geographical areas, namely West Africa, Central-East Africa, and South America. Two subgroups were defined in West Africa, a genotypic group circulating in the sylvatic zone of the western part of Africa, from western Ivory Coast-Mali to Senegal, and a group responsible for large outbreaks from eastern Ivory Coast-Burkina Faso to Cameroon. Strains from Central-East Africa showed a low ratio of transition: transversion of about 1 instead of 8 to 10 for other strains, when their nucleotide sequences were compared with those of other African strains. This may reflect a more distant relationship between the former strains and the others. No change was observed in the highly conserved amino acid domain encompassing the TGD sequence, an important determinant of flavivirus tropism and pathogenesis. Our results support earlier observations on the genetic relationships between YF isolates established by T1 oligonucleotide fingerprinting and offer a useful tool for the understanding of YF virus distribution and evolution.

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1994-02-01
2024-03-28
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References

  1. Ballinger-Crabtree M. E., Miller B. R. 1990; Partial nucleotide sequence of South American yellow fever virus strain 1899/81: structural proteins and NS1. Journal of General Virology 71:2115–2121
    [Google Scholar]
  2. Barrett A. D. T. 1987; Yellow fever vaccines. Bulletin de l’Institut Pasteur 85:103–124
    [Google Scholar]
  3. Brès P., Deubel V., Digoutte J. P. 1983; Les Togaviridae: le virus de la fievre jaune. In Virologie Médicale pp. 570–585 Maurin J. Edited by Paris: Flammarion;
    [Google Scholar]
  4. Chu M. C., O’Rourke E. J., Trent D. W. 1989; Genetic relatedness among structural protein genes of dengue 1 virus strains. Journal of General Virology 70:1701–1712
    [Google Scholar]
  5. Clarke D. 1960; Antigenic analysis of certain group B arthropod- borne viruses by antibody adsorption. Journal of Experimental Medicine 111:21–32
    [Google Scholar]
  6. Deubel V., Pailliez J. P., Cornet M., Schlesinger J. J., Diop M., Digoutte J. -P., Girard M. 1985; Homogeneity among Senegalese strains of yellow fever virus. American Journal of Tropical Medicine and Hygiene 34:976–983
    [Google Scholar]
  7. Deubel V., Digoutte J. -P., Monath T. P., Girard M. 1986a; Genetic heterogeneity of yellow fever virus strains from Africa and the Americas. Journal of General Virology 67:209–213
    [Google Scholar]
  8. Deubel V., Ekue E. K., Diop M., Diop A., Digoutte J. -P. 1986b; Introduction à l’analyse de la virulence du virus de la fièvre jaune (Flaviviridae): études génétiques, immunochimiques et bio-logiques comparatives entre les souches atténuées vaccinates et leurs souches parentales. Annales de I’Institut Pasteur / Virologie 137E:181–192
    [Google Scholar]
  9. Deubel V., Schlesinger J. J., Digoutte J. -P., Girard M. 1987; Comparative immunochemical and biochemical analysis of African and South American yellow fever viruses. Archives of Virology 94:331–338
    [Google Scholar]
  10. Deubel V., Laille M., Hugnot J. P., Chungue E., Guesdon J. L., Drouet M. T., Bassot S., Chevrier D. 1990; Identification of dengue sequences by genomic amplification: rapid diagnosis of dengue serotypes in peripheral blood. Journal of Virological Methods 30:41–54
    [Google Scholar]
  11. Deubel V., Nogueira R. M., Drouet M. T., Zeller H., Reynes J. M., Ha D. Q. 1993; Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses. Archives of Virology 129:197–210
    [Google Scholar]
  12. Germain M., Cornet M., Mouchet J., Monath T. P., Herve J. P., Salaun J. J., Cordellier R., Saluzzo J. F., Camicas J. L., Hervy J. P., Robert V., Deubel V., Gonzales J. P., Digoutte J. P., Darwish D. O. 1982; Recent advances in research regarding sylvatic yellow fever in West and Central Africa. Bulletin de I’Institut Pasteur 80:315–330
    [Google Scholar]
  13. Hahn C. S., Dalrymple J. M., Strauss J. H., Rice C. M. 1987; Comparison of the virulent Asibi strain of yellow fever virus with the 17D vaccine strain derived from it. Proceedings of the National Academy of Sciences, U.S.A 84:2019–2023
    [Google Scholar]
  14. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73:237–244
    [Google Scholar]
  15. Jennings A. D., Whitby J. E., Minor P. D., Barrett A. D. T. 1993; Comparison of the nucleotide and deduced amino acid sequences of the envelope protein genes of the wild-type French viscerotropic strain of yellow fever virus and the live vaccine strain, French neurotropic vaccine, derived from it. Virology 192:692–695
    [Google Scholar]
  16. Kerschner J. H., Vorndam A. V., Monath T. P., Trent D. W. 1986; Genetic and epidemiologic studies of dengue type 2 viruses by hybridization using synthetic deoxyoligonucleotides as probes. Journal of General Virology 67:2645–2661
    [Google Scholar]
  17. Lobigs M., Usha R., Nestorowicz A., Marshall I. D., Weir R. C., Dalgarno L. 1990; Host selection of Murray Valley encephalitis virus variants altered at an RGD sequence in the envelope protein and in mouse virulence. Virology 176:587–595
    [Google Scholar]
  18. McCrae A. W. A., Kiry B. J. 1982; Yellow fever and Zika virus epizootics and enzootics in Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene 76:552–562
    [Google Scholar]
  19. Mandl C. W., Guirakhoo F., Holzmann H., Heinz F. X., Kunz C. 1989; Antigenic structure of the flavivirus envelope protein E at the molecular level, using tick-borne encephalitis virus as a model. Journal of Virology 63:564–571
    [Google Scholar]
  20. Mégret F., Hugnot J. P., Falconar A., Gentry M. K., Morens D. M., Murray J. M., Schlesinger J. J., Wright P. J., Young P., Van Regenmortel M. H. V., Deubel V. 1992; Use of fusion recombinant proteins and monoclonal antibodies to define linear and discontinuous antigenic sites on the dengue virus envelope glycoprotein. Virology 187:480–491
    [Google Scholar]
  21. Monath T. P. 1990; Flaviviruses. In Virology, 2nd edn.. pp. 763–814 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  22. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implication for virus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  23. Rico-Hesse R. 1990; Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology 174:479–493
    [Google Scholar]
  24. Saluzzo J. F., Herve J. P., Salaun J. J., Cornet J. P., Camicas J. L., Heme G., Robin Y. 1980; Caractéristiques des souches de virus de la fièvre jaune isolées des oeufs et des larves d’une tique Amblyomma variegatum recoltée sur le bétail à Bangui (Centr- afrique). Annales de I’Institut Pasteur / Virologie 131E:155–165
    [Google Scholar]
  25. Schlesinger J. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125:8–17
    [Google Scholar]
  26. Sérié C., Andral L., Poirier A., Lindrec A. 1968; Etudes sur la fievre jaune en Ethiopie. 5: Etudes epidemiologiques. Bulletin of the World Health Organization 38:879–884
    [Google Scholar]
  27. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy: The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  28. Who 1989; Yellow fever in 1987. Weekly Epidemiological Record 64:37–14
    [Google Scholar]
  29. Who 1991; Yellow fever in 1989 and 1990. Weekly Epidemiological Record 11:76–77
    [Google Scholar]
  30. Who 1992; Yellow fever epidemic in Cameroon, 1990. Weekly Epidemiological Record 67:245–252
    [Google Scholar]
  31. Wilbur W. J., Lipman D. J. 1983; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences U.S.A. 80:726–730
    [Google Scholar]
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