1887

Abstract

One-day-old ducklings experimentally infected with duck hepatitis B virus (DHBV) were found to be immunologically tolerant to virus antigens (DHBsAg, DHBcAg), with no humoral or cellular immune responses being detected. When immunized with virus antigens in Freund’s complete adjuvant, no immune responses could be induced. Rabbit anti-DHBs sera were complexed to a solid matrix ( Cowan A strain) and purified DHBsAg was bound to this complex to form a solid matrix antibody-antigen (SMAA) complex. This SMAA was used as an immunogen to immunize the tolerant ducks. After three injections, in 12 of 17 ducks serum DHBV DNA became absent and serum DHBsAg was cleared. In eight of 16 ducks, low titres of anti-DHBs could be detected. The SMAA approach shows potential for application in immunoregulatory treatment for chronically infected hepatitis B patients.

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1994-02-01
2021-10-16
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References

  1. Beasley R. P., Hwang L. Y. 1984; Epidemiology of hepatocellular carcinoma. In Viral Hepatitis and Liver Diseases pp. 209–224 Vyas G. N., Dienstag J. L., Hoofnagle J. H. Edited by Orlando: Grune & Stratton;
    [Google Scholar]
  2. Celis E., Chang T. W. 1984; Antibodies to hepatitis B surface antigen potentiate the response of human T lymphocyte clones to the same antigen. Science 224:297–299
    [Google Scholar]
  3. Celis E., Abraham K. G., Miller R. W. 1987; Modulation of immunological response to hepatitis B virus by antibodies. Hepatology 7:563–568
    [Google Scholar]
  4. Fei G. Z., Lou H. Z., Yao G. B., Wen Y. M. 1989; Interferon-gamma induced by purified hepatitis B surface antigen in HBV vaccinees. National Medical Journal of China 69:271–273
    [Google Scholar]
  5. Gust I. D., Burrell C. J., Coulep A. G., Robinson W. S., Zuckerman A. J. 1986; Taxonomic classification of human hepatitis B virus. Intervirology 25:14–29
    [Google Scholar]
  6. Halpern M. S., Mason W. S., Coates L., O’Connell A. P., England J. M. 1987; Humoral immunoresponsiveness in duck hepatitis B virus infected ducks. Journal of Virology 61:916–920
    [Google Scholar]
  7. Higgins D. A., Tech C. S. H. 1988; Duck lymphocytes II. Culture conditions for optimum transformation response to phytohemagglutinin. Journal of Immunological Methods 106:135–145
    [Google Scholar]
  8. Hu C. D., Zhang W., Xu Y. Y., Zhou B. Y., Luo Y. F., Wen Y. M. 1992; Studies on core antigen and antibody of duck hepatitis B virus. Chinese Journal of Virology 8:19–25
    [Google Scholar]
  9. Jamieson B. D., Ahmad R. 1988; T-cell tolerance: exposure to virus in utero does not cause a permanent deletion of specific T cells. Proceedings of the National Academv of Sciences U.S.A: 852265–2268
    [Google Scholar]
  10. Li Z. L., Zhang W., Luo Y. F., Wen Y. M. 1989; Study on the infectivity of duck hepatitis B virus surface antibody complex. Chinese Journal of Experimental and Clinical Virology 3:6–10
    [Google Scholar]
  11. Marion P. L., Knight S. S., Feitelson M. A., Oshiro L. S., Robinson W. S. 1983; Major polypeptide of duck hepatitis B surface antigen particles. Journal of Virology 48:534–541
    [Google Scholar]
  12. Maruyama T., Mclachlan A., Iino S., Koike K., Yasuda K., Milich D. R. 1992; Serologic evidence of ongoing immune response in asymptomatic and symptomatic hepatitis B carriers. 1992 Molecular Biology of Hepatitis B Virus Meeting San Diego: p 132: Abstract
    [Google Scholar]
  13. Penna A., Chisari F. V., Bertoletti A., Missale G., Fowler P., Giuberti T., Ficcadori F., Ferrari C. 1992; Cytotoxic T lymphocytes recognize an HLA-A2-restricted epitope within the hepatitis B virus nucleocapsid antigen. Journal of Experimental Medicine 174:1565–1570
    [Google Scholar]
  14. Qu D., Zhai W. R., Lin F. Q., Wen Y. M. 1988; Bursectomy enhances duck hepatitis B virus replication in liver. In Viral Hepatitis and Liver Diseases pp. 503–505 Zuckerman A. J. Edited by New York: Alan R.Liss;
    [Google Scholar]
  15. Randall R. E., Young D. F. 1988; Humoral and cytotoxic T cell immune responses to internal and external structural proteins of simian virus 5 induced by immunization with solid matrix-antibody- antigen complexes. Journal of General Virology 69:2505–2516
    [Google Scholar]
  16. Randall R. E., Young D. F. 1991; Solid matrix-antibody- antigen complexes induce specific CD8+ cells that clear a persistent paramyxovirus infection. Journal of Virology 65:719–726
    [Google Scholar]
  17. Randall R. E., Young D. F., Southern J. A. 1988; Immunization with solid matrix-antibody-antigen complexes containing surface or internal virus structural proteins protects mice from infection with the paramyxovirus, simian virus 5. Journal of General Virologv 69:2517–2526
    [Google Scholar]
  18. Wen Y. M., Zhang W., Xu Y. Y., Luo Y. F., Li P. Y. 1992; Elimination of immune tolerance to hepatitis virus in an animal model. Chinese Medical Journal 105:199–203
    [Google Scholar]
  19. Zhang W., Wen Y. M., Li Z. L., Luo Y. F. 1988; A simple and rapid method for duck hepatitis B virus antigen and antibody assay. Acta Academia Medicinae Shanghai 15:413–416
    [Google Scholar]
  20. Zhang H. X., Zhang W., Luo Y. F., Wen Y. M. 1990; Studies on blastogenesis of duck peripheral blood lymphocytes. Shanghai Journal of Immunology 10:262–264
    [Google Scholar]
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