1887

Abstract

Respiratory syncytial viruses (RSVs) cause serious respiratory tract disease in infants and children worldwide and similar disease in calves. Strains have been isolated from other ruminant animals such as sheep and goats, but these viruses have not been characterized at the molecular level. In this study, we report the cloning and sequencing of four structural genes coding for the phosphoprotein, nucleocapsid (N) protein, matrix (M) protein and 22K protein of an ovine RSV strain. Comparisons of these sequences with those of bovine and human RSV show that the M and N proteins are the most conserved between ruminant RSV strains and the N protein is the most conserved protein between human and ruminant RSV strains. The attachment G glycoprotein and the small hydrophobic protein are the most divergent proteins among human and ruminant RSV subgroups.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-12-3597
1994-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/12/JV0750123597.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-12-3597&mimeType=html&fmt=ahah

References

  1. Alansari H., Potgieter L. N. D. 1993; Nucleotide sequence analysis of the ovine respiratory syncytial virus G glycoprotein. Virology 196:873–877
    [Google Scholar]
  2. Alansari H., Potgieter L. N. D. 1994; Nucleotide and predicted amino acid sequence analysis of the ovine respiratory syncytial virus non-structural 1C and IB genes and the small hydrophobic protein gene. Journal of General Virology 75:401–404
    [Google Scholar]
  3. Amann V. L., Lerch R. A., Anderson K., Wertz G. 1992; Bovine respiratory syncytial virus nucleocapsid protein mRNA sequence analysis and expression from recombinant vaccinia virus vectors. Journal of Virology 73:999–1003
    [Google Scholar]
  4. Collins P. L., Wertz G. W. 1985; The envelope-associated 22K protein of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polytranscript. Journal of Virology 54:65–71
    [Google Scholar]
  5. Collins P. L., Anderson K., Lange S. J., Wertz G. 1985; Correct sequence for the major nucleocapsid protein mRNA of respiratory syncytial virus. Virology 146:69–77
    [Google Scholar]
  6. Collins P. L., Olmsted R. A., Johnson P. R. 1990a; The small hydrophobic protein of human respiratory syncytial virus: comparison between antigenic subgroups A and B. Journal of General Virology 71:1571–1576
    [Google Scholar]
  7. Collins P. L., Hill M. G., Johnson P. R. 1990b; The two open reading frames of the 22K mRNA of human respiratory syncytial virus: sequence comparison of antigenic subgroups A and B and expression in vitro . Journal of General Virology 71:3015–3020
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  9. Frohman M. A., Dush N. K., Martin G. R. 1988; Rapid production of full length cDNAs from rare transcripts: amplification using single gene specific oligonucleotide primer. Proceedings of the National Academy of Sciences, U.S.A 85:8998–9002
    [Google Scholar]
  10. Furze J., Wertz G., Lerch R., Tayler G. 1994; Antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus. Journal of General Virology 75:363–370
    [Google Scholar]
  11. Gouli S. A., Ahmed K. 1991; A procedure for rapid isolation of cloned cDNA inserts using PCR technique. BioTechniques 10:306–308
    [Google Scholar]
  12. Johnson P. R., Collins P. L. 1989; The IB (NS2) and 1C (NS1) and N proteins of human respiratory syncytial virus (RSV) of antigenic subgroups A and B: sequence conservation and divergence within RSV genomic RNA. Journal of General Virology 70:1539–1547
    [Google Scholar]
  13. Johnson P. R., Collins P. L. 1990; Sequence comparison of the phosphoprotein mRNAs of antigenic subgroups A and B of human respiratory syncytial virus identifies a highly divergent domain in the predicted protein. Journal of General Virology 71:481–485
    [Google Scholar]
  14. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987; The G glycoprotein of human respiratory syncytial viruses of subgroup A and B: extensive sequence divergence between anti-genically related proteins. Proceedings of the National Academy of Sciences, U.S.A 84:5625–5629
    [Google Scholar]
  15. Lambden P. R. 1985; Nucleotide sequence of the respiratory syncytial virus phosphoprotein gene. Journal of General Virology 66:1607–1612
    [Google Scholar]
  16. LeaMaster B. R., Everman J. F., Mueller M. K., Prieur M. K., Schlei J. V. 1983; Serologic and virologic studies on naturally occurring respiratory syncytial virus and Haemophilus sonmnus infections in sheep. American Association of Veterinary Laboratory Diagnosticians 26:265–276
    [Google Scholar]
  17. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  18. Örvell C., Norrby E., Mufson M. A. 1987; Preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup B. Journal of General Virology 68:3125–3135
    [Google Scholar]
  19. Samal S. K., Zamora M. 1991; Nucleotide sequence analysis of a matrix and small hydrophobic protein dicistronic mRNA of bovine respiratory syncytial virus demonstrates extensive sequence divergence of the small hydrophobic protein from that of human respiratory syncytial virus. Journal of General Virology 72:1715–1720
    [Google Scholar]
  20. Samal S. K., Zamora M., Mcpillipa T. H., Mohanty S. B. 1991; Molecular cloning and sequence analysis of bovine respiratory syncytial virus mRNA encoding the major nucleocapsid protein. Virology 180:453–456
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  22. Satake M., Venkatesan S. 1984; Nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein. Journal of Virology 50:92–99
    [Google Scholar]
  23. Smith M. H., Lehmkuhl H. D., Phillips S. M. 1979; Isolation and characterization of respiratory syncytial virus from goats. American Association of Veterinary Laboratory Diagnosticians 22:259–269
    [Google Scholar]
  24. Zamora M., Samal S. K. 1992; Sequence analysis of M2 mRNA of bovine respiratory syncytial virus obtained from an F-M2 dicistronic mRNA suggests structural homology with that of human respiratory syncytial virus. Journal of General Virology 73:737–741
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-12-3597
Loading
/content/journal/jgv/10.1099/0022-1317-75-12-3597
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error