1887

Abstract

W16B is a temperature-sensitive mutant of vesicular stomatitis virus. Others have shown that it is temperature-sensitive for replication and for transcription and that these phenotypes are probably due to mutation of the N (nucleocapsid) gene. Five independent revertants were isolated from W16B based on their ability to grow at 39 °C. The thermosensitivity of transcription by these revertants was similar to that of the wild-type virus [ (HR) ] from which W16B was derived. Fractionation-reconstitution studies of two revertants indicated that the reversion was in the N or P (phosphoprotein) gene. The N and P genes of (HR), W16B, and these two revertants were sequenced. There were no differences between the P genes. Comparison of the predicted N protein sequences of (HR), W16B and the two revertants indicated that the growth and transcription phenotypes of ttW16B were due to a change of amino acid residue 238 from threonine to isoleucine. The amino acid at position 238 in the other three revertants also showed an exact reversion to threonine. Amino acid residue 238 lies in a domain of the N protein which is highly conserved among vesiculoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-12-3591
1994-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/12/JV0750123591.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-12-3591&mimeType=html&fmt=ahah

References

  1. Banerjee A. K., Barik S. 1992; Gene expression of vesicular stomatitis virus genome RNA. Virology 188:417–428
    [Google Scholar]
  2. Barr J., Chambers P., Pringle C. R., Easton A. J. 1991; Sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleocapsid proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and filoviruses. Journal of General Virology 72:677–685
    [Google Scholar]
  3. Beckes J. D., Haller A. A., Perrault J. 1987; Differential effect of ATP concentration on synthesis of vesicular stomatitis virus leader RNAs and mRNAs. Journal of Virology 61:3470–3478
    [Google Scholar]
  4. Bernard J., Bremont M., Winton J. 1992; Nucleocapsid gene sequence of a North American isolate of viral haemorrhagicsepticaemia virus, a fish rhabdovirus. Journal of General Virology 73:1011–1014
    [Google Scholar]
  5. Bernard J., Lecocq-Xhonneux F., Rossius M., Thiry M. E., de Kinkelin P. 1990; Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagicsepticaemia virus. Journal of General Virology 11:1669–1674
    [Google Scholar]
  6. Bourhy H., Kissi B., Tordo N. 1993; Molecular diversity of the Lyssavirus genus. Virology 194:70–81
    [Google Scholar]
  7. Bras F., Teninges D., Dezelee S. 1994; Sequences of the N and M genes of the sigma virus of Drosophila and evolutionary comparison. Virology 200:189–199
    [Google Scholar]
  8. Cairns J. E., Holloway A. F., Cormack D. V. 1972; Temperature-sensitive mutants of vesicular stomatitis virus: in vitro studies of virion-associated polymerase. Journal of Virology 10:1130–1135
    [Google Scholar]
  9. Cormack D. V., Sword H., Holloway A. F. 1975; Progeny resulting from complementation between mutants of vesicular stomatitis virus. Canadian Journal of Microbiology 21:230–233
    [Google Scholar]
  10. Crysler J. G., Lee P., Reinders M., Prevec L. 1990; The sequence of the nucleocapsid protein (N) gene of Piry virus: possible domains in the N protein of vesiculoviruses. Journal of General Virology 71:2191–2194
    [Google Scholar]
  11. Davis N. L., Arnheiter H., Wertz G. W. 1986; Vesicular stomatitis virus N and NS proteins form multiple complexes. Journal of Virology 59:751–754
    [Google Scholar]
  12. Gilmore R. D. Jr Leong J. C. 1988; The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virology 167:644–648
    [Google Scholar]
  13. Holloway A. F., Wong P. K. Y., Cormack D. V. 1970; Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus. Virology 42:917–926
    [Google Scholar]
  14. Howard M., Wertz G. 1989; Vesicular stomatitis virus RNA replication: a role for the NS protein. Journal of General Virology 70:2683–2694
    [Google Scholar]
  15. Hunt D. M., Hutchinson K. L. 1993; Amino acid changes in the L polymerase protein of vesicular stomatitis virus which confer aberrant polyadenylation and temperature-sensitive phenotypes. Virology 193:786–793
    [Google Scholar]
  16. Hunt D. M., Wagner R. R. 1974; Location of the transcription defect in Group I temperature-sensitive mutants of vesicular stomatitis virus. Journal of Virology 13:28–35
    [Google Scholar]
  17. Hutchinson K. L., Bouknight D. P., Fan W., Hunt D. M. 1990; Revertants of a mutant of vesicular stomatitis virus which has an aberrant polyadenylation activity and a temperature-sensitive transcriptase. Virology 174:444–449
    [Google Scholar]
  18. Leppert M., Rittenhouse L., Perrault J., Summers D. F., Kolakofsky D. 1979; Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell 18:735–747
    [Google Scholar]
  19. Masters P. S., Banerjee A. K. 1987; Sequences of Chandipura virus N and NS genes: evidence for high mutability of the NS gene within vesiculoviruses. Virology 157:298–306
    [Google Scholar]
  20. Masters P. S., Banerjee A. K. 1988; Complex formation with vesicular stomatitis virus phosphoprotein NS prevents binding of nucleocapsid protein N to non-specific RNA. Journal of Virology 62:2658–2664
    [Google Scholar]
  21. Moyer S. A. 1989; Replication of the genome RNAs of defective interfering particles of vesicular stomatitis and Sendai viruses using heterologous viral proteins. Virology 172:341–345
    [Google Scholar]
  22. Ngan J. S. C., Holloway A. F., Cormack D. V. 1974; Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of Group I and Group IV mutants. Journal of Virology 14:765–772
    [Google Scholar]
  23. Peluso R. W., Moyer S. A. 1984; Vesicular stomatitis virus proteins required for the in vitro replication of defective interfering particle genome RNA. In Nonsegmented Negative Strand Viruses pp. 153–160 Bishop D. H. L., Compans R. W. Edited by New York: Academic Press;
    [Google Scholar]
  24. Peluso R. W., Moyer S. A. 1988; Viral proteins required for the in vitro replication of vesicular stomatitis virus defective interfering particle genome RNA. Virology 162:369–376
    [Google Scholar]
  25. Perrault J., Mclear P. W. 1984; ATP dependence of vesicular stomatitis virus transcription initiation and modulation by mutation in the nucleocapsid protein. Journal of Virology 51:635–642
    [Google Scholar]
  26. Pringle C. R. 1987; Rhabdovirus genetics. In The Rhabdoviruses pp. 167–243 Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  27. Rose J., Schubert M. 1987; Rhabdovirus genomes and their products. In The Rhabdoviruses pp. 129–166 Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  28. Sanchez A., Kiley M. P., Klenk H.-D., Feldmann H. 1992; Sequence analysis of the Marburg virus nucleoprotein gene: comparison to Ebola virus and other non-segmented RNA viruses. Journal of General Virology 73:347–357
    [Google Scholar]
  29. Takacs A. M., Das T., Banerjee A. K. 1993; Mapping of interacting domains between the nucleocapsid protein and the phosphoprotein of vesicular stomatitis virus by using a two-hybrid system. Proceedings of the National Academy of Sciences, U.S.A 90:10375–10379
    [Google Scholar]
  30. Thomas D. L., Newcomb W. W., Brown J. C., Wall J. S., Hamfeld J. F., Trus B. L., Steven A. C. 1985; Mass and molecular composition of vesicular stomatitis virus: a scanning transmission electron microscopy analysis. Journal of Virology 54:598–607
    [Google Scholar]
  31. Wertz G. W., Davis N. L., Patton J. 1987; The role of proteins in vesicular stomatitis virus replication. In The Rhabdoviruses pp. 271–296 Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  32. Wong P. K. Y., Holloway A. F., Cormack D. V. 1972; Characterization of three complementation groups of vesicular stomatitis virus. Virology 50:829–840
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-12-3591
Loading
/content/journal/jgv/10.1099/0022-1317-75-12-3591
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error