Some properties of the particles of citrus ringspot virus (CtRSV) and the related citrus psorosis-associated virus (CPsAV) are described. The particles of CtRSV have been reported to be sinuous linear structures about 10 nm in diameter and of two lengths (300 to 500 nm and 1500 to 2500 nm) representing ‘top’ and ‘bottom’ sedimentation components. We show that these particles are collapsed double-stranded forms of nucleocapsid-like, highly flexuous open circles formed of filaments 3 to 4 nm in diameter. Top-component filaments had contour lengths of 600 to 1000 nm, i.e. twice that reported for the corresponding collapsed form. Bottom-component filaments had contour lengths about four times longer than those of top-component filaments. The structures suggest that CtRSV represents a new genus (possibly family) related to the tenuiviruses. However, we failed to demonstrate any serological relationship between CtRSV and several tenuiviruses; moreover, the capsid protein sizes and host ranges are quite different. We offer the name Ophiovirus for the proposed new genus.
da GracaJ. V.,
LeeR. F.,
MorenoP.,
CweroloE. L.,
DerrickK. S.1991; Comparison of isolates of citrus ringspot, psorosis, and other viruslike agents of citrus. Plant Disease 75:613–616
DerrickK. S.,
LeeR. F.,
HewittB. G.1992; Spiroviruses: a new group of plant viruses associated with citrus psorosis and ringspot. In Abstracts of the XII International Conference on Citrus Virology New Delhi: 1992 86
DerrickK. S.,
BrlanskyR. H.,
da GracaJ. V.,
LeeR. F.,
TimmerL. W.,
NguyenT. K.1988; Partial characterization of a virus associated with citrus ringspot. Phytopathology 78:1298–1301
DerrickK. S.,
LeeR. F.,
HewittB. G.,
BartheG. A.,
da GracaJ. V.1991; Characterization of citrus ringspot virus. In Proceedings of the 11th Conference of the International Organization of Citrus Virologists, Orlando, Fla1989 pp 386–390BrlanskyR. H.,
LeeR. F.,
TimmerL. W.
Edited by University of California, Riverside: IOCV;
EspinozaA. M.,
HernándezM.,
PereiraR.,
FalkB.,
MedinaV.1992; In situ immunogold labeling analysis of the rice hojablanca virus nucleoprotein and major noncapsid protein. Virology 191:619–627
FranckiR. I. B.,
FauquetC. M.,
KnudsenD. L.,
BrownF.editor1991; Classification and Nomenclature of Viruses: Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Suppl 2
GarcíaM. L.,
ArreseE. L.,
GrauO.,
SarachuA. N.1991a; Citrus psorosis disease agent behaves as a two component ssRNA virus. In Proceedings of the 11th Conference of the International Organization of Citrus Virologists, Orlando, Fla1989 pp. 337–344BrlanskyR. H.,
LeeR. F.,
TimmerL. W.
Edited by University of California, Riverside: IOCV;
GarnseyS. M.,
TimmerL. W.1980; Mechanical transmissibility of citrus ringspot virus isolates from Florida, Texas, and California. In Proceedings of the 8th Conference of the International Organization of Citrus Virologists, Sydney1979174–179CalavanE. C.,
GarnseyS. M.,
TimmerL. W.
Edited by University of California, Riverside: IOCV;
HuietL.,
FeldsteinP. A.,
TsaiJ. H.,
FalkB. W.1993; The maize stripe virus major noncapsid protein messenger RNA transcripts contain heterogeneous leader sequences at their 5′ termini. Virology 197:808–812
KoenigR.,
BurgermeisterW.1986; Applications of immuno-blotting in plant virus diagnosis. In Developments and Applications in Virus Testing pp. 121–137JonesR. A. C.,
TorranceL.
Edited by Wellesbourne, U.K: Association of Applied Biologists;
MilneR. G.1993a; Solid phase immune electron microscopy of virus preparations. In Immuno-Gold Electron Microscopy pp. 25–70HyattA. D.,
EatonB. T.
Edited by Boca Raton, Fla: CRC Press;
MilneR. G.1993b; Electron microscopy of in vitro preparations. In Diagnosis of Plant Virus Diseases pp. 215–251MatthewsR. E. F.
Edited by Boca Raton, Fla: CRC Press;
Navas-CastilloJ.,
MorenoP.,
CambraM.,
DerrickK. S.1993; Partial purification of a virus associated with a Spanish isolate of citrus ringspot. Plant Pathology 42:339–346