1887

Abstract

A study of the capsid proteins of different legume-infecting potyviruses using specific monoclonal antibodies on immunoblots of crude extracts from infected plants revealed that cowpea aphid-borne mosaic virus (CAMV) and blackeye cowpea mosaic virus (B1CMV) have coat protein values of 32K and 35K, respectively. Immunoblot comparisons of BICMV, peanut stripe mosaic virus (PStV), bean common mosaic virus (BCMV) and azuki bean mosaic virus (AzMV) revealed equal reactivity of their 35K coat proteins. Similar comparisons between CAMV and the necrotic strain of BCMV (isolate NL3) showed a serological relationship between their 32K coat proteins, results providing the first evidence of a possible similarity between CAMV and BCMV NL3. Peptides from trypsin digests of the coat proteins of several of these legume-infecting potyviruses were analysed by HPLC. Comparison of the peptide profiles confirmed the serological results in distinguishing the two subgroups. Peptide profiles of coat protein from BICMV, PStV, AzMV and BCMV were almost identical, results suggesting that they could be considered as strains of one virus. In contrast, peptide profiles of various CAMV serotypes and BCMV NL3 were distinct from the first group and exhibited limited similarities to each other.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-12-3555
1994-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/12/JV0750123555.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-12-3555&mimeType=html&fmt=ahah

References

  1. Behncken G. M., Maleevsky L. 1977; Detection of cowpea aphid-borne mosaic virus in Queensland. Australian Journal of Experimental Agriculture and Animal Husbandry 17:674–678
    [Google Scholar]
  2. Bos L. 1970; The identification of three new viruses isolated from wisteria and pisum in The Netherlands, and the problem of variation within the potato virus Y group. Netherlands Journal of Plant Pathology 76:8–46
    [Google Scholar]
  3. Commandeur U., Koenig R., Lesemann D.-E., Torrance L., Burgermeister W., Liu Y., Schots A., Alric M., Grassi G. 1992; Epitope mapping on fragments of beet necrotic yellow vein virus coat protein. Journal of General Virology 73:695–700
    [Google Scholar]
  4. Donald R. G. K., Zhou H., Jackson A. O. 1993; Serological analysis of barley stripe mosaic virus-encoded proteins in infected barley. Virology 195:659–668
    [Google Scholar]
  5. Drijfhout E., Bos L. 1977; The identification of two new strains of bean common mosaic virus. Netherlands Journal of Plant Pathology 83:13–25
    [Google Scholar]
  6. Hasselmann D., Lesemann D.-E., Vetten H. J. 1993; Epitope analysis of the capsid proteins of three related potyviruses using monoclonal antibodies. Vlth International Congress of Plant Pathology, Montreal Abstract 17.1.1
    [Google Scholar]
  7. Huguenot C., Furneaux M. T., Thottappilly G., Rossel H. W., Hamilton R. I. 1993; Evidence that cowpea aphid-borne mosaic and blackeye cowpea mosaic viruses are two different potyviruses. Journal of General Virology 74:335–340
    [Google Scholar]
  8. Iwaki M., Roechan M., Tantera D. M. 1975; Virus diseases of legume plants in Indonesia. 1. Cowpea aphid-borne mosaic virus. Contributions of the Central Research Institute for Agriculture, Bogor 13:1–14
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Lima J. A. A., Purcifull D. E., Hiebert E. 1979; Purification, partial characterization, and serology of blackeye cowpea mosaic virus. Phytopathology 69:1252–1258
    [Google Scholar]
  11. Lovisolo O., Conti M. 1966; Identification of an aphid-transmitted cowpea mosaic virus. Netherlands Journal of Plant Pathology 72:265–269
    [Google Scholar]
  12. Mckern N. M., Whittaker L. A., Strike P. M., Ford R. E., Jensen S. G., Shukla D. D. 1990; Coat protein properties indicate that maize dwarf mosaic virus KS1 is a strain of Johnsongrass mosaic virus. Phytopathology 80:907–912
    [Google Scholar]
  13. McKern N. M., Mink G. I., Barnett O. W., Mishra A., Whittaker L. A., Silbernagel M. J., Ward C. W., Shukla D. D. 1992a; Isolates of bean common mosaic virus comprising two distinct potyviruses. Phytopathology 82:923–929
    [Google Scholar]
  14. Mckern N. M., Shukla D. D., Barnett O. W., Vetten H. J., Dijkstra J., Whittaker L. W., Ward C. W. 1992b; Coat protein properties suggest that adzuki bean mosaic virus, blackeye cowpea mosaic virus, peanut stripe mosaic virus, and three isolates from soybean are all strains of the same potyvirus. Intervirology 33:121–134
    [Google Scholar]
  15. Mali V. R., Kulthe K. S. 1980; A seedborne potyvirus causing mosaic of cowpea in India. Plant Disease 64:925–928
    [Google Scholar]
  16. Mink G. I., Silbernagel M. J. 1992; Serological and biological relationships among viruses in the bean common mosaic subgroup. In Potyvirus Taxonomy Archives of Virology Supplementum 5 397–406
    [Google Scholar]
  17. Pio-Ribeiro G., Kuhn W. 1980; Cowpea stunt: heterogeneous and differential reaction of cowpea cultivars. Phytopathology 70:244–249
    [Google Scholar]
  18. Shukla D. D., Inglis A. S., Mckern N. M., Gough K. H. 1986; Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152:118–125
    [Google Scholar]
  19. Shukla D. D., Mckern N. M., Gough K. S., Tracy S. L., Letho S. G. 1988; Differentiation of potyviruses and their strains by high performance liquid chromatographic peptide profiling of coat proteins. Journal of General Virology 69:493–502
    [Google Scholar]
  20. Shukla D. D., Ward C. W. 1989; Structure of potyvirus coat proteins and its application in taxonomy of the potyvirus group. Advances in Virus Research 36:273–314
    [Google Scholar]
  21. Taiwo M. A., Gonsalves D., Provvidenti R., Thurston H. D. 1982; Partial characterization and grouping of isolates of blackeye cowpea mosaic and cowpea aphidborne mosaic viruses. Phytopathology 72:590–596
    [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences U.S.A.: 76:4350–4354
    [Google Scholar]
  23. Vetten H. J., Lesemann D. E., Maiss E. 1992; Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. In Potyvirus Taxonomy Archives of Virology Supplementum 5 415–434
    [Google Scholar]
  24. Zettler F. W., Evans I. R. 1972; Blackeye cowpea mosaic virus in Florida: host range and incidence in certified cowpea seed. Proceedings of the Florida State Horticultural Society 85:99–101
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-12-3555
Loading
/content/journal/jgv/10.1099/0022-1317-75-12-3555
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error