1887

Abstract

The GenBank accession numbers for the sequence data reported in this paper are U14736 to U14744.

We have sequenced the coat protein gene of nine isolates of papaya ringspot virus (PRSV) including six Australian and three Asian isolates and compared these with four previously reported sequences of PRSV. There was up to 12 % sequence variation between isolates at the nucleotide level. However, there was no significant difference between the sequences obtained from Australian isolates irrespective of whether they were PRSV type P (cucurbit or papaya infecting) or PRSV type W (cucurbit infecting) and these isolates were more closely related to one another than to any other isolate. These results imply that PRSV-P, first recorded in Australia in 1991, arose locally from PRSV-W (first recorded in Australia in 1978) rather than being introduced. Further, there was no consistent sequence difference between PRSV-P and PRSV-W isolates that would obviously account for their host range difference.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-12-3547
1994-12-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/12/JV0750123547.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-12-3547&mimeType=html&fmt=ahah

References

  1. Abel P. P., Nelson R. S., Barun D., Hoffmann N., Rogers S. G., Fraley R. T., Beachy R. N. 1986; Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–233
    [Google Scholar]
  2. Bateson M. F., Dale J. 1992; The nucleotide sequence of the coat protein gene and 3′ untranslated region of papaya ringspot virus type W(Aust). Archives of Virology 123:101–109
    [Google Scholar]
  3. Brault V., Candresse T., Le Gall O., Delbos R. P., Lanneau M., Dunez J. 1993; Genetically engineered resistance against grapevine chrome mosaic nepovirus. Plant Molecular Biology 21:89–97
    [Google Scholar]
  4. Da Camara Machado M. L., Da Camara Machado A., Hanzer V., Weiss H., Regner F., Steinkellner H., Mattanovich D., Plail R., Knapp E., Kalthoff B., Katinger H. 1992; Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of plum pox virus. Plant Cell Reports 11:25–29
    [Google Scholar]
  5. Devereaux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  6. Fitch M. M., Manshardt R. M., Gonsalves D., Slightom J. L., Sanford J. C. 1992; Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus. Bio/Technology 10:1466–1472
    [Google Scholar]
  7. Greber R. S. 1978; Watermelon mosaic virus 1 and 2 in Queensland cucurbit crops. Australian Journal of Agricultural Research 29:1235–1245
    [Google Scholar]
  8. Hattori M., Sakaki Y. 1986; Dideoxy sequencing method using denatured plasmid templates. Analytical Biochemistry 152:232–238
    [Google Scholar]
  9. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  10. Higgins D. G., Bleasly A., Fuchs R. 1991; Clustal V: improved software for multiple sequence alignment. CABIOS 8:189–191
    [Google Scholar]
  11. Kaniewski W., Lawson C., Sammons B., Haley L., Hart J., Delannay X., Tumer N. E. 1990; Field resistance of transgenic russet burbank potato to effects of infection by potato virus X and potato virus Y. Bio/Technology 8:750–754
    [Google Scholar]
  12. Ling K., Namba S., Gonsalves C., Slightom J. L., Gonsalves D. 1991; Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papaya ringspot virus coat protein gene. Bio/Technology 9:752–758
    [Google Scholar]
  13. Mackenzie D. J., Tremaine J. H. 1990; Transgenic Nicotiana debneyii expressing viral coat protein are resistant to potato virus S infection. Journal of General Virology 71:2167–2170
    [Google Scholar]
  14. Nakajima M., Hayakawa T., Nakamura I., Suzuki M. 1993; Protection against cucumber mosaic virus (CMV) strains O and Y and chrysanthemum mild mottle virus in transgenic tobacco plants expressing CMV-O coat protein. Journal of General Virology 74:319–322
    [Google Scholar]
  15. Namba S., Ling K., Gonsalves C., Slightom J. L., Gonsalves D. 1992; Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses. Phytopathology 82:940–946
    [Google Scholar]
  16. Nei M., Maruyama T., Chakraborty R. 1975; The bottleneck effect and genetic variability in populations. Evolution 29:1–10
    [Google Scholar]
  17. Nelson R. S., McCormick S. M., Delannay X., Dube P., Layton J., Anderson E. J., Kaniewska M., Proksch R. K., Horsch R. B., Rogers S. G., Fraley R. T., Beachy R. N. 1988; Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/ Technology 8:403–409
    [Google Scholar]
  18. Quemada H., L’Hostis B., Gonsalves D., Reardon I. M., Heinrik-Son R., Hiebert E. L., Sieu L. C., Slightom J. L. 1990; The nucleotide sequences of the 3′-terminal regions of papaya ringspot virus strains W and P. Journal of General Virology 71:203–210
    [Google Scholar]
  19. Quemada H. D., Gonsalves D., Slightom J. L. 1991; Expression of coat protein gene from cucumber mosaic virus strain C in tobacco, protection against infections by CMV strains transmitted mechanically or by aphids. Phytopathology 81:794–802
    [Google Scholar]
  20. Robertson N. L., French R., Gray S. M. 1991; Use of group-specific primers and the polymerase chain reaction for the detection and identification of luteovires. Journal of General Virology 72:1473–1477
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  22. Sanders P. R., Sammons B., Kaniewski W., Haley L., Layton J., Lavallee B. J., Delannay X., Tumer N. E. 1992; Field resistance of transgenic tomatoes expressing the tobacco mosaic virus or tomato mosaic virus coat protein genes. Phytopathology 82:683–690
    [Google Scholar]
  23. Stark D. M., Beachy R. N. 1989; Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 1:1257–1261
    [Google Scholar]
  24. Shukla D. D., Ward C. W. 1989; Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Advances in Virology 36:273–314
    [Google Scholar]
  25. Shukla D. D., Strike P. M., Tracy S. L., Gough K. H., Ward C. W. 1998; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
    [Google Scholar]
  26. Swofford D. L. 1991 PAUP, Phylogenetic analysis under parsimony, version 3.0s Computer program distributed by the Illinois Natural History Survey, Champaign, Illinois, U.S.A.:
    [Google Scholar]
  27. Tennant P., Gonsalves C., Fitch M., Manshardt R., Slightom J. L., Gonsalves D. 1993; Comparative reactions of transgenic papaya expressing the coat protein gene of papaya ringspot virus from Hawaii against challenge inoculations with isolates from different geographic regions. Abstracts of the 6th International Congress of Plant Pathology Montreal, Canada:
    [Google Scholar]
  28. Thomas J. E., Dodman R. L. 1993; The first record of papaya ringspot virus-type P in Australia. Australasian Plant Pathology 22:12–7
    [Google Scholar]
  29. Wang C.-H., Yeh S.-D. 1992; Nucleotide sequence comparison of the 3′-terminal regions of severe, mild and non-papaya infecting strains of papaya ringspot virus. Archives of Virology 127:345–354
    [Google Scholar]
  30. Xiao X.-W., Frenkel M. J., Teakle D. S., Ward C. W., Shukla D. D. 1993; Sequence diversity in the surface-exposed N-terminal region of the coat proteins of several strains of sugarcane mosaic virus correalates with their host range. Abstracts of the 6th International Congress of Plant Pathology Montreal, Canada:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-12-3547
Loading
/content/journal/jgv/10.1099/0022-1317-75-12-3547
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error