1887

Abstract

Hepatitis C virus (HCV) type K3a (type 3a), which represents a minor genotype in Europe, the U.S.A. and Asia, appears to be significantly distributed throughout Australia and Brazil. We amplified the HCV-K3a/650 genome by reverse transcription polymerase chain reaction in ten overlapping fragments and determined the nucleotide sequences. The total sequence was 9454 bases in length and contained an open reading frame of 3021 amino acids, which is 10 or 11 amino acids longer than in HCV type 1 and 12 amino acids shorter than the sequence of type 2. These differences were due to the different lengths of both the putative envelope protein E2 and the NS5A regions, whose nucleotide lengths differ between types 1 and 2 also. Phylogenetic analysis of the putative core region and a portion of NS5B encoding the Gly-Asp-Asp motif indicated that HCV- K3a closely matched the corresponding type 3a group. The deletion and addition of amino acids in both E2 and NS5A may be associated with their pathobiological features.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-11-3279
1994-11-01
2022-05-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/11/JV0750113279.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-11-3279&mimeType=html&fmt=ahah

References

  1. Bukh J., Purcell H., Miller R. H. 1992; Sequence analysis of the 5′ noncoding region of hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A. 89:4942–4946
    [Google Scholar]
  2. Bukh J., Purcell H., Miller R. H. 1993; At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative El gene of isolates collected worldwide. Proceedings of the National Academy of Sciences, U.S.A 90:8234–8238
    [Google Scholar]
  3. Cha T. -A., Beall E., Irvine B., Kolberg J., Chien D., Kuo G., Urdea M. S. 1992; At least five related, but distinct, hepatitis C viral genotypes exist. Proceedings of the National Academy of Sciences, U.S.A 89:7144–7148
    [Google Scholar]
  4. Chambers T. J., Hahn C. S., Galler R., Rice C. M. 1990; Flavivirus genome organization, expression, and replication. Annual Review of Microbiology 44:100–109
    [Google Scholar]
  5. Chan S. -W., McOmish F., Holmes E. C., Dow B., Peutherer J. F., Follett E., Yap P. L., Simmonds P. 1992; Analysis of a new hepatitis C virus type and its phylogenetic relationship to existing variants. Journal of General Virology 13:1131–1141
    [Google Scholar]
  6. Choo Q. -L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA derived from a blood- borne non-A, non-B hepatitis genome. Science 244:359–362
    [Google Scholar]
  7. Choo Q. -L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby R., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A 88:2451–2455
    [Google Scholar]
  8. Enomoto N., Takada A., Nakao T., Date T. 1990; There are two major types of hepatitis C virus in Japan. Biochemical and Biophysical Research Communications 170:1021–1025
    [Google Scholar]
  9. Grakoui A., Wychowski C., Lin C., Feinstone S. M., Rice C. M. 1993a; Expression and identification of hepatitis C virus polyprotein cleavage products. Journal of Virology 67:1385–1395
    [Google Scholar]
  10. Grakoui A., McCourt D. W., Wychowski C., Feinstone S. M., Rice C. M. 1993b; Characterization of the hepatitis C virus- encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. Journal of Virology 67:2832–2843
    [Google Scholar]
  11. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Shimotohno K. 1991a; Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proceedings of the National Academy of Sciences, U.S.A 88:5547–5551
    [Google Scholar]
  12. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Ohkoshi S., Shimotohno K. 1991b; Hypervariable regions in the putative glycoprotein of hepatitis C virus. Biochemical and Biophysical Research Communications 175:220–228
    [Google Scholar]
  13. Hijikata M., Mizushima H., Akagi T., Mori S., Kakiuchi N., Kato N., Tanaka T., Kimura K., Shimotohno K. 1993; Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. Journal of Virology 67:4665–4675
    [Google Scholar]
  14. Kanai K., Kako M., Okamoto H. 1992; HCV genotypes in chronic hepatitis C and response to interferon. Lancet 339:1543
    [Google Scholar]
  15. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohokoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, U.S.A 87:9524–9528
    [Google Scholar]
  16. Lee C., Cheng C., Wang J., Lumeng L. 1992; Identification of hepatitis C virus with a non-conserved sequence of the 5′ untrans–lated region. Journal of Clinical Microbiology 30:1602–1604
    [Google Scholar]
  17. Manabe S., Fuke I., Akagi T., Tanishita O., Kaii C., Gomi Y., Yoshida S., Mori C., Takamizawa A., Yoshida I., Okayama H. 1994; Production of nonstructural proteins of hepatitis C viral protease encoded by NS3. Virology 198:636–644
    [Google Scholar]
  18. Mori S., Kato N., Yagyu A., Tanaka T., Ikeda Y., Petchclai B., Chiewsilp P., Kurimura T., Shimotohno K. 1992; A new type of hepatitis C virus in patients in Thailand. Biochemical and Biophysical Research Communications 183:334–342
    [Google Scholar]
  19. Nakao T., Enomoto N., Takada N., Takada A., Date T. 1991; Typing of hepatitis C virus by restriction fragment length polymorphism. Journal of General Virology 72:2105–2112
    [Google Scholar]
  20. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. 1990; The 5′-terminal sequence of the hepatitis C virus genome. Japanese Journal of Experimental Medicine 60:167–177
    [Google Scholar]
  21. Okamoto H., Okada S., Sugiyama Y., Kurai K., Iizuka H., Machida A., Miyakawa Y., Mayumi M. 1991; Nucleotide sequence of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions. Journal of General Virology 72:2697–2704
    [Google Scholar]
  22. Okamoto H., Kurai K., Okada S., Yamamoto K., Iizuka H., Tanaka T., Fukuda S., Tsuda F., Mishiro S. 1992; Full- length sequence of a hepatitis C virus genome having poor homology to reported isolates: comparative study of four distinct genotypes. Virology 188:331–341
    [Google Scholar]
  23. Poch O., Sauvaget I., Delarue M., Tordo N. 1989; Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO Journal 8:3867–3874
    [Google Scholar]
  24. Simmonds P., Holmes E. C., Cha T. -A., Chan S. -W., McOmish F., Irvine B., Beall E., Yap P. L., Kolberg J., Urdea M. S. 1993a; Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. Journal of General Virology 74:2391–2399
    [Google Scholar]
  25. Simmonds P., McOmish F., Yap P. L., Chan S. -W., Lin C. K., Dusheiko G., Saeed A. A., Holmes E. C. 1993b; Sequence variability in the 5′-non-coding region of hepatitis C virus: identification of a new virus type and restrictions on sequence diversity. Journal of General Virology 74:661–668
    [Google Scholar]
  26. Takada N., Takase S., Takada A., Date T. 1992a; HCV genotypes in different countries. Lancet 339:808
    [Google Scholar]
  27. Takada N., Takase S., Enomoto N., Takada A., Date T. 1992b; Clinical backgrounds of the patients having different types of hepatitis C virus genomes. Journal of Hepatology 14:35–10
    [Google Scholar]
  28. Takada N., Takase S., Takada A., Date T. 1993a; Differences in the hepatitis C virus genotypes in different countries. Journal of Hepatology 17:277–283
    [Google Scholar]
  29. Takada N., Matsuda Y., Takase S., Takada A., Date T. 1993b; New genotype of hepatitis C virus. Gastroenterologia Japonica 28:323
    [Google Scholar]
  30. Takamizawa A., Mori C., Fuke I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
  31. Tsutsumi M., Urashima S., Takada A., Date T., Tanaka Y. 1994; Detection of antigenes related to hepatitis C virus RNA encoding the NS5 region in the liver of patients with chronic type C hepatitis. Hepatology 19:265–272
    [Google Scholar]
  32. Yoshioka K., Kakumu S., Wakita, Ishikawa Y., Itoh Y., Takayanagi M., Higashi Y., Shibata M., Morishima T. 1992; Detection of hepatitis C virus by polymerase chain reaction and response to interferon-α therapy: relationship to genotypes of hepatitis C virus. Hepatology 16:293–299
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-11-3279
Loading
/content/journal/jgv/10.1099/0022-1317-75-11-3279
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error