1887

Abstract

Human T cell leukaemia virus type I (HTLV-I) provirus DNA was found to be methylated in patients with adult T cell leukaemia. We have therefore examined the possibility that DNA methylation might contribute to HTLV-I latency. methylation of HTLV-I long terminal repeat (LTR)-chloramphenicol acetyltransfer- ase or LTR-Luciferase constructs at eight II sites, a subset of the eukaryotic methylation site CpG, resulted in a three- to fourfold inhibition of transcription in transfected cells. Inhibition of transcription by methylation of all CpG methylation sites using Sxsl methylase was much more pronounced (50- to 80-fold). As partial methylation of the LTR showed, methylation of the promoter region was responsible for most of the effect. Whereas cellular stimulation by a combination of phorbol 12-myristate 13-acetate and Tax was able to reverse the II methylation effect, the inhibition by SsjI methylation was not suppressible under these conditions. The results are in line with a possible function of DNA methylation in HTLV-I latency.

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1994-11-01
2024-12-06
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