1887

Abstract

DNA sequence analysis of the genome of the Smith strain of murine cytomegalovirus (MCMV) revealed an open reading frame (ORF) with amino acid sequence identity to glycoprotein L (gL) of other herpesviruses. The ORF is 822 bp in size and has the capacity to encode a protein of 274 amino acids. It has significant identity with the gL genes of human CMV and human herpesvirus 6. The coding sequence of the gL gene of MCMV strain K181 was also determined, and expressed in as a fusion protein with glutathione S- transferase using the pGEX expression system. Two antibody-binding regions were identified on the basis of the reactivity of a series of truncated gL constructs with anti-MCMV antibodies. One was mapped to residues 1 to 38 and the other between residues 230 and 274. Polyclonal antibodies specific to gL were raised against the lull-length gL fusion protein. The antisera were shown to react with a 46K protein present in purified virions by Western blotting. Treatment of purified virions with endoglycosidase-H or -F resulted in reductions in of the 46K species to 42K and 3IK, respectively. The antisera did not exhibit any neutralizing activity in a plaque reduction assay.

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1994-11-01
2022-05-19
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References

  1. Britt W. J., Vugler L., Butfiloski E. J., Stephens E. B. 1990; Cell surface expression of human cytomegalovirus (HCMV) gp55- 116 (gB): use of HCMV-recombinant vaccinia virus-infected cells in analysis of the human neutralizing antibody response. Journal of Virology 64:1079–1085
    [Google Scholar]
  2. Chee M. S., Banker A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A. III Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; Analysis of the protein coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  3. Cranage M. P., Kouzarides T., Bankier A. T., Satchwell S., Weston K., Tomlinson P., Barrell B., Hart H., Bell S. E., Minson A. C., Smith G. L. 1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO Journal 5:3057–3063
    [Google Scholar]
  4. Cranage M. P., Smith G. L., Bell S. E., Hart H., Brown C., Bankier A. T., Tomlinson P., Barrell B. G., Minson T. C. 1988; Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H. Journal of Virology 62:1416–1422
    [Google Scholar]
  5. Elliott R., Clark C., Jaquish D., Spector D. H. 1991; Transcription analysis and sequence of the putative murine cytomegalovirus DNA polymerase gene. Virology 185:169–186
    [Google Scholar]
  6. Farrell H. E., Shellam G. R. 1990; Characterization of neutralizing monoclonal antibodies to murine cytomegalovirus. Journal of General Virology 71:655–664
    [Google Scholar]
  7. Forghani B., Ni L., Grose C. 1994; Neutralizing epitope of the varicella-zoster virus gH:gL glycoprotein complex. Virology 199:458–462
    [Google Scholar]
  8. Gretch D. R., Kari B., Rasmussen L., Gehrz R. C., Stinski M. F. 1988; Identification and characterization of three distinct families of glycoprotein complexes in the envelopes of human cytomegalovirus. Journal of Virology 62:875–881
    [Google Scholar]
  9. Hudson J. B. 1979; The murine cytomegalovirus as a model for the study of viral pathogenesis and persistent infections. Archives of Virology 62:1–29
    [Google Scholar]
  10. Hutchinson L., Browne H., Wargent V., Davis-Poynter N., Primorac S., Goldsmith K., Minson A. C., Johnson D. C. 1992; A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH. Journal of Virology 66:2240–2250
    [Google Scholar]
  11. Kaye J. F., Gompels U. A., Minson A. C. 1992; Glycoprotein H of human cytomegalovirus (HCMV) forms a stable complex with the HCMV UL115 gene product. Journal of General Virology 73:2693–2698
    [Google Scholar]
  12. Keay S., Baldwin B. 1991; Anti-idiotype antibodies that mimic gp86 of human cytomegalovirus inhibit viral fusion but not attachment. Journal of Virology 65:5124–5128
    [Google Scholar]
  13. Keay S., Merigan T. C., Rasmussen L. 1989; Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus. Proceedings of the National Academy of Sciences, U.S.A 86:10100–10103
    [Google Scholar]
  14. Kim K. S., Sapienza V. J., Carp R. I., Moon H. M. 1976; Analysis of structural proteins of purified murine cytomegalovirus. Journal of Virology 17:906–915
    [Google Scholar]
  15. Liu D. X., Gompels U. A., Foa-Tomasi L., Campadelli-Fiume G. 1993a; Human herpesvirus-6 glycoprotein H and L homologs are components of the gp100 complex and the gH external domain is the target for neutralizing monoclonal antibodies. Virology 197:12–22
    [Google Scholar]
  16. Liu D. X., Gompels U. A., Nicholas J., Lelliott C. 1993b; Identification and expression of the human herpesvirus 6 glycoprotein H and interaction with an accessory 40K glycoprotein. Journal of General Virology 74:1847–1857
    [Google Scholar]
  17. Loh L. C. 1989; Synthesis and processing of a 22–26K murine cytomegalovirus glycoprotein recognized by a neutralizing monoclonal antibody. Virology 169:474–478
    [Google Scholar]
  18. Loh L. C., Qualtiere L. F. 1988; A neutralizing monoclonal antibody recognizes an 87K envelope glycoprotein on the murine cytomegalovirus virion. Virology 162:498–502
    [Google Scholar]
  19. Loh L. C., Balachandran N., Qualtiere L. F. 1988; Characterization of a major virion envelope glycoprotein complex of murine cytomegalovirus and its immunological cross-reactivity with human cytomegalovirus. Virology 166:206–216
    [Google Scholar]
  20. Rapp M., Messerle M., Buhler B., Tannheimer M., Keil G. M., Koszinowski U. H. 1992; Identification of the murine cytomegalovirus glycoprotein B gene and its expression by recombinant vaccinia virus. Journal of Virology 66:4399–4406
    [Google Scholar]
  21. Rapp M., Lŭcin P., Messerle M., Loh L. C., Koszinowski U. H. 1994; Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus. Journal of General Virology 75:183–188
    [Google Scholar]
  22. Rasmussen L. 1990; Immune response to human cytomegalovirus infection. Current Topics in Microbiology and Immunology 154:221–254
    [Google Scholar]
  23. Roop C., Hutchinson L., Johnson D. C. 1993; A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein H. Journal of Virology 67:2285–2297
    [Google Scholar]
  24. Schlesinger M. J., Schlesinger S. 1987; Domains of virus glycoproteins. Advances in Virus Research 33:1–44
    [Google Scholar]
  25. Smith D. B., Johnson K. S. 1988; Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31–40
    [Google Scholar]
  26. Spaete R. R., Perot K., Scott P. I., Nelson J. A., Stinski M. F., Pachl C. 1993; Coexpression of truncated human cytomegalovirus gH with the UL115 gene product or the truncated human fibroblast growth factor receptor results in transport of gH to the cell surface. Virology 193:853–861
    [Google Scholar]
  27. Urban M., Britt W., Mach M. 1992; The dominant linear neutralizing antibody-binding site of glycoprotein gp86 of human cytomegalovirus is strain specific. Journal of Virology 66:1303–1311
    [Google Scholar]
  28. Van Regenmortel M. H. V. 1986; Which structural features determine protein antigenicity. Trends in Biochemical Sciences 11:36–39
    [Google Scholar]
  29. Xu J., Dallas P. B., Lyons P. A., Shellam G. R., Scalzo A. A. 1992; Identification of the glycoprotein H gene of murine cytomegalovirus. Journal of General Virology 73:1849–1854
    [Google Scholar]
  30. Xu J., Scalzo A. A., Lyons P. A., Shellam G. R. 1994; Expression of the glycoprotein H of murine cytomegalovirus and identification of an N-terminal antibody-binding region. Virology in press
    [Google Scholar]
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